Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.

This study investigates a novel strategy combining ultrashort pulsed-direct laser interference patterning (USP-DLIP) and chemical etching to create hierarchically micro- and nanorough topographies on zirconia with improved cell-instructive and antibacterial properties. Linear (L3) and grid (G3) micr...

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Autores: Garcia-de-Albeniz N, Müller DW, Mücklich F, Ginebra MP, Jiménez-Piqué E, Mas-Moruno C
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p29689
Acceso en línea:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29689
Access Level:acceso abierto
Palabra clave:Antibacterial
Chemical etching
Dental implants
Laser patterning
Nanotopography
Osteointegration
Topography
Zirconia
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spelling Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.Garcia-de-Albeniz NMüller DWMücklich FGinebra MPJiménez-Piqué EMas-Moruno CAntibacterialChemical etchingDental implantsLaser patterningNanotopographyOsteointegrationTopographyZirconiaThis study investigates a novel strategy combining ultrashort pulsed-direct laser interference patterning (USP-DLIP) and chemical etching to create hierarchically micro- and nanorough topographies on zirconia with improved cell-instructive and antibacterial properties. Linear (L3) and grid (G3) micropatterns of 3 µm periodicity were fabricated via USP-DLIP, and subsequently treated with hydrofluoric acid to introduce an additional homogeneous nanotopography across the patterns. The individual and combined effects of micropatterning and etching on biological responses were evaluated using human mesenchymal stem cells (hMSCs) and two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus) in mono- and co-culture settings. Micropatterns primarily guided cell morphology, alignment, and migration, while the introduced nanotopography enhanced focal adhesion formation and modulated cell-surface interactions. Antibacterial effects were found to be topography- and species-specific: micropatterns restricted P. aeruginosa colonization through a bacterial confinement mechanism, whereas etching-induced nanotopography effectively reduced S. aureus adhesion by limiting contact points. In co-culture assays, hMSC survival depended on a complex interplay between cell spreading and bacterial retention, dictated by surface features. Notably, chemical etching improved the antibacterial potential of the patterns against S. aureus, but it did not result in a synergistic improvement on cellular responses. Indeed, among all tested surfaces, the non-etched linear pattern (L3) consistently exhibited the most favorable outcomes -enhancing hMSC adhesion, migration, and osteogenic differentiation and mineralization, while reducing bacterial colonization and supporting cell survival under infection-like co-culture conditions.ELSEVIER2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29689MATERIALS TODAY BIOISSN: 25900064reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p296892026-05-27T12:37:41Z
dc.title.none.fl_str_mv Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
title Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
spellingShingle Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
Garcia-de-Albeniz N
Antibacterial
Chemical etching
Dental implants
Laser patterning
Nanotopography
Osteointegration
Topography
Zirconia
title_short Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
title_full Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
title_fullStr Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
title_full_unstemmed Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
title_sort Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
dc.creator.none.fl_str_mv Garcia-de-Albeniz N
Müller DW
Mücklich F
Ginebra MP
Jiménez-Piqué E
Mas-Moruno C
author Garcia-de-Albeniz N
author_facet Garcia-de-Albeniz N
Müller DW
Mücklich F
Ginebra MP
Jiménez-Piqué E
Mas-Moruno C
author_role author
author2 Müller DW
Mücklich F
Ginebra MP
Jiménez-Piqué E
Mas-Moruno C
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antibacterial
Chemical etching
Dental implants
Laser patterning
Nanotopography
Osteointegration
Topography
Zirconia
topic Antibacterial
Chemical etching
Dental implants
Laser patterning
Nanotopography
Osteointegration
Topography
Zirconia
description This study investigates a novel strategy combining ultrashort pulsed-direct laser interference patterning (USP-DLIP) and chemical etching to create hierarchically micro- and nanorough topographies on zirconia with improved cell-instructive and antibacterial properties. Linear (L3) and grid (G3) micropatterns of 3 µm periodicity were fabricated via USP-DLIP, and subsequently treated with hydrofluoric acid to introduce an additional homogeneous nanotopography across the patterns. The individual and combined effects of micropatterning and etching on biological responses were evaluated using human mesenchymal stem cells (hMSCs) and two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus) in mono- and co-culture settings. Micropatterns primarily guided cell morphology, alignment, and migration, while the introduced nanotopography enhanced focal adhesion formation and modulated cell-surface interactions. Antibacterial effects were found to be topography- and species-specific: micropatterns restricted P. aeruginosa colonization through a bacterial confinement mechanism, whereas etching-induced nanotopography effectively reduced S. aureus adhesion by limiting contact points. In co-culture assays, hMSC survival depended on a complex interplay between cell spreading and bacterial retention, dictated by surface features. Notably, chemical etching improved the antibacterial potential of the patterns against S. aureus, but it did not result in a synergistic improvement on cellular responses. Indeed, among all tested surfaces, the non-etched linear pattern (L3) consistently exhibited the most favorable outcomes -enhancing hMSC adhesion, migration, and osteogenic differentiation and mineralization, while reducing bacterial colonization and supporting cell survival under infection-like co-culture conditions.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29689
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29689
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv MATERIALS TODAY BIO
ISSN: 25900064
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
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