Combined Use of the Ab105-2phiDeltaCI Lytic Mutant Phage and Different Antibiotics in Clinical Isolates of Multi-Resistant Acinetobacter baumannii

Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phiDeltaCI) that displayed antimicrobial activity against A. baumannii clinical strain Ab177_GEIH-2000 (isola...

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Detalles Bibliográficos
Autores: Blasco Otero, Lucía, Ambroa Abalo, Antón, Lopez, Maria, Fernández García, Laura, Bleriot Rial, Ines Maria, TRASTOY PENA, ROCIO, Ramos-Vivas, Jose, Coenye, Tom, Fernandez-Cuenca, Felipe, Vila, Jordi, Martinez-Martinez, Luis, Rodriguez-Bano, Jesus, Pascual, Alvaro, Cisneros, Jose Miguel, Pachon, Jeronimo, Bou Arévalo, Germán, Tomás Carmona, María del Mar
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/15892
Acceso en línea:https://www.ncbi.nlm.nih.gov/pubmed/31726694
http://hdl.handle.net/20.500.11940/15892
Access Level:acceso abierto
Palabra clave:Acinetobacter baumannii
CHUAC
INIBIC
Descripción
Sumario:Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phiDeltaCI) that displayed antimicrobial activity against A. baumannii clinical strain Ab177_GEIH-2000 (isolated in the GEIH-REIPI Spanish Multicenter A. baumannii Study II 2000/2010, Umbrella Genbank Bioproject PRJNA422585, and for which meropenem and imipenem MICs of respectively, 32 microg/mL, and 16 microg/mL were obtained). We observed an in vitro synergistic antimicrobial effect (reduction of 4 log-7 log CFU/mL) between meropenem and the lytic phage in all combinations analyzed (Ab105-2phiDeltaCI mutant at 0.1, 1 and 10 MOI and meropenem at 1/4 and 1/8 MIC). Moreover, bacterial growth was reduced by 8 log CFU/mL for the combination of imipenem at 1/4 MIC plus lytic phage (Ab105-2phiDeltaCI mutant) and by 4 log CFU/mL for the combination of imipenem at 1/8 MIC plus lytic phage (Ab105-2phiDeltaCI mutant) at both MOI 1 and 10. These results were confirmed in an in vivo model (G. mellonella), and the combination of imipenem and mutant Ab105-2phiDeltaCI was most effective (p < 0.05). This approach could help to reduce the emergence of phage resistant bacteria and restore sensitivity to antibiotics used to combat multi-resistant strains of Acinetobacter baumannii.

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