Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.

Giardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causin...

ver descrição completa

Detalhes bibliográficos
Autores: Rafiei, Abdollah, Baghlaninezhad, Raheleh, Panabad, Esmat, Beiromvand, Molouk, Köster, Pamela Carolina, Bailo-Barroso, Begoña, Hernandez-De-Mingo, Marta, Carmena, David
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/10486
Acesso em linha:http://hdl.handle.net/20.500.12105/10486
Access Level:acceso abierto
Palavra-chave:Multilocus Sequence Typing
Adolescent
Adult
Aged
Child
Child, Preschool
Cytoskeletal Proteins
Female
Genotype
Giardia lamblia
Giardiasis
Glutamate Dehydrogenase
Humans
Iran
Male
Middle Aged
Phylogeny
Protozoan Proteins
Triose-Phosphate Isomerase
Young Adult
id ES_e31bd1e89a5fad4e9dc212823eb51fbe
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/10486
network_acronym_str ES
network_name_str España
repository_id_str
spelling Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.Rafiei, AbdollahBaghlaninezhad, RahelehPanabad, EsmatBeiromvand, MoloukKöster, Pamela CarolinaBailo-Barroso, BegoñaHernandez-De-Mingo, MartaCarmena, DavidMultilocus Sequence TypingAdolescentAdultAgedChildChild, PreschoolCytoskeletal ProteinsFemaleGenotypeGiardia lambliaGiardiasisGlutamate DehydrogenaseHumansIranMaleMiddle AgedPhylogenyProtozoan ProteinsTriose-Phosphate IsomeraseYoung AdultGiardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causing human infections in Shushtar County, Southwestern Iran. Individual faecal specimens were collected from 1,163 individuals (male/female ratio: 0.9; age range 2-75 years) with (n = 258) and without (n = 905) gastrointestinal symptoms living in rural and urban settings during the period 2017-2018. Conventional (sucrose flotation and microscopy) methods were used for the initial detection of G. duodenalis cysts in faecal specimens. Microscopy-positive samples were confirmed by PCR amplification and sequencing of the small subunit rRNA (ssu rRNA) gene of the parasite. A multilocus genotyping (MLG) scheme targeting the triose phosphate isomerase (tpi), the glutamate dehydrogenase (gdh), and the beta-giardin (bg) genes was used for genotyping purposes. Giardia duodenalis cysts were detected in 7.7% (90/1,163) of samples by microscopy, of which 82 were confirmed by ssu-PCR. Successful amplification and sequencing results were obtained for 23.2% (19/82), 9.8% (8/82), and 8.5% (7/82) of the confirmed samples at the tpi, gdh, and bg loci, respectively. MLG data for the three loci were available for two samples only. Out of the 24 samples genotyped at any loci, 50% (12/24) were identified as assemblage A and the remaining half as assemblage B. Overall, AII was the most prevalent sub-assemblage detected (41.7%, 10/24), followed by BIII (25.0%, 6/24), discordant BIII/BIV (5/24) or AII/AIII (2/24) sequences, and BIV (1/24). No significant correlation was demonstrated between a given assemblage/sub-assemblage and the occurrence of clinical symptoms. No genotypes adapted to animal hosts other than humans (e.g. assemblages C-F) were found circulating in the investigated human population, suggesting that transmission of human giardiasis in this Iranian region is primarily of anthroponotic nature. Further molecular-based studies are needed to confirm and expand these results, and to ascertain the presence and public health relevance of the parasite in environmental (e.g. drinking water) samples.Public Library of Science (PLOS)Instituto de Salud Carlos III20202020-06-1820202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/10486reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/104862026-06-12T12:43:37Z
dc.title.none.fl_str_mv Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
title Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
spellingShingle Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
Rafiei, Abdollah
Multilocus Sequence Typing
Adolescent
Adult
Aged
Child
Child, Preschool
Cytoskeletal Proteins
Female
Genotype
Giardia lamblia
Giardiasis
Glutamate Dehydrogenase
Humans
Iran
Male
Middle Aged
Phylogeny
Protozoan Proteins
Triose-Phosphate Isomerase
Young Adult
title_short Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
title_full Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
title_fullStr Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
title_full_unstemmed Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
title_sort Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
dc.creator.none.fl_str_mv Rafiei, Abdollah
Baghlaninezhad, Raheleh
Panabad, Esmat
Beiromvand, Molouk
Köster, Pamela Carolina
Bailo-Barroso, Begoña
Hernandez-De-Mingo, Marta
Carmena, David
author Rafiei, Abdollah
author_facet Rafiei, Abdollah
Baghlaninezhad, Raheleh
Panabad, Esmat
Beiromvand, Molouk
Köster, Pamela Carolina
Bailo-Barroso, Begoña
Hernandez-De-Mingo, Marta
Carmena, David
author_role author
author2 Baghlaninezhad, Raheleh
Panabad, Esmat
Beiromvand, Molouk
Köster, Pamela Carolina
Bailo-Barroso, Begoña
Hernandez-De-Mingo, Marta
Carmena, David
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Multilocus Sequence Typing
Adolescent
Adult
Aged
Child
Child, Preschool
Cytoskeletal Proteins
Female
Genotype
Giardia lamblia
Giardiasis
Glutamate Dehydrogenase
Humans
Iran
Male
Middle Aged
Phylogeny
Protozoan Proteins
Triose-Phosphate Isomerase
Young Adult
topic Multilocus Sequence Typing
Adolescent
Adult
Aged
Child
Child, Preschool
Cytoskeletal Proteins
Female
Genotype
Giardia lamblia
Giardiasis
Glutamate Dehydrogenase
Humans
Iran
Male
Middle Aged
Phylogeny
Protozoan Proteins
Triose-Phosphate Isomerase
Young Adult
description Giardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causing human infections in Shushtar County, Southwestern Iran. Individual faecal specimens were collected from 1,163 individuals (male/female ratio: 0.9; age range 2-75 years) with (n = 258) and without (n = 905) gastrointestinal symptoms living in rural and urban settings during the period 2017-2018. Conventional (sucrose flotation and microscopy) methods were used for the initial detection of G. duodenalis cysts in faecal specimens. Microscopy-positive samples were confirmed by PCR amplification and sequencing of the small subunit rRNA (ssu rRNA) gene of the parasite. A multilocus genotyping (MLG) scheme targeting the triose phosphate isomerase (tpi), the glutamate dehydrogenase (gdh), and the beta-giardin (bg) genes was used for genotyping purposes. Giardia duodenalis cysts were detected in 7.7% (90/1,163) of samples by microscopy, of which 82 were confirmed by ssu-PCR. Successful amplification and sequencing results were obtained for 23.2% (19/82), 9.8% (8/82), and 8.5% (7/82) of the confirmed samples at the tpi, gdh, and bg loci, respectively. MLG data for the three loci were available for two samples only. Out of the 24 samples genotyped at any loci, 50% (12/24) were identified as assemblage A and the remaining half as assemblage B. Overall, AII was the most prevalent sub-assemblage detected (41.7%, 10/24), followed by BIII (25.0%, 6/24), discordant BIII/BIV (5/24) or AII/AIII (2/24) sequences, and BIV (1/24). No significant correlation was demonstrated between a given assemblage/sub-assemblage and the occurrence of clinical symptoms. No genotypes adapted to animal hosts other than humans (e.g. assemblages C-F) were found circulating in the investigated human population, suggesting that transmission of human giardiasis in this Iranian region is primarily of anthroponotic nature. Further molecular-based studies are needed to confirm and expand these results, and to ascertain the presence and public health relevance of the parasite in environmental (e.g. drinking water) samples.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-06-18
2020
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/10486
url http://hdl.handle.net/20.500.12105/10486
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422471621378048
score 15.81155

Registos relacionados