Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis

Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a br...

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Autores: Menshawy, Ahmed M S, Hosein, Hosein I, García Benzaquén, Nerea, Martínez Alares, Irene, Sayour, Ashraf E, Goyache Goñi, Joaquín, Azzam, Ragab A A, Álvarez Sánchez, Julio, Domínguez Rodríguez, Lucas José, Pérez Sancho, Marta, García-Seco Romero, María Teresa
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/35281
Acceso en línea:https://hdl.handle.net/20.500.14352/35281
Access Level:acceso abierto
Palabra clave:Veterinaria
3109 Ciencias Veterinarias
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spelling Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysisMenshawy, Ahmed M SHosein, Hosein IGarcía Benzaquén, NereaMartínez Alares, IreneSayour, Ashraf EGoyache Goñi, JoaquínAzzam, Ragab A AÁlvarez Sánchez, JulioDomínguez Rodríguez, Lucas JoséPérez Sancho, MartaGarcía-Seco Romero, María TeresaVeterinaria3109 Ciencias VeterinariasBrucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region.Hindawi Publishing CorporationPérez Sancho, MartaUniversidad Complutense de Madrid20142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/35281reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/352812026-06-02T12:44:21Z
dc.title.none.fl_str_mv Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
title Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
spellingShingle Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
Menshawy, Ahmed M S
Veterinaria
3109 Ciencias Veterinarias
title_short Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
title_full Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
title_fullStr Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
title_full_unstemmed Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
title_sort Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis
dc.creator.none.fl_str_mv Menshawy, Ahmed M S
Hosein, Hosein I
García Benzaquén, Nerea
Martínez Alares, Irene
Sayour, Ashraf E
Goyache Goñi, Joaquín
Azzam, Ragab A A
Álvarez Sánchez, Julio
Domínguez Rodríguez, Lucas José
Pérez Sancho, Marta
García-Seco Romero, María Teresa
author Menshawy, Ahmed M S
author_facet Menshawy, Ahmed M S
Hosein, Hosein I
García Benzaquén, Nerea
Martínez Alares, Irene
Sayour, Ashraf E
Goyache Goñi, Joaquín
Azzam, Ragab A A
Álvarez Sánchez, Julio
Domínguez Rodríguez, Lucas José
Pérez Sancho, Marta
García-Seco Romero, María Teresa
author_role author
author2 Hosein, Hosein I
García Benzaquén, Nerea
Martínez Alares, Irene
Sayour, Ashraf E
Goyache Goñi, Joaquín
Azzam, Ragab A A
Álvarez Sánchez, Julio
Domínguez Rodríguez, Lucas José
Pérez Sancho, Marta
García-Seco Romero, María Teresa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Pérez Sancho, Marta
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Veterinaria
3109 Ciencias Veterinarias
topic Veterinaria
3109 Ciencias Veterinarias
description Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/35281
url https://hdl.handle.net/20.500.14352/35281
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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