The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production

The red yeast X. dendrorhous is one of the few natural sources of astaxanthin, a carotenoid used in aquaculture for salmonid fish pigmentation and in the cosmetic and pharmaceutical industries for its antioxidant properties. Genetic control of carotenogenesis is well characterized in this yeast; how...

Descripción completa

Detalles Bibliográficos
Autores: Alcaíno, Jennifer, Bravo, Natalia, Cordova, Pamela, Marcoleta, Andrés, Contreras, Gabriela, Barahona, Salvador, Sepúlveda, Dionisia, Fernández Lobato, María, Baeza, Marcelo, Cifuentes, Víctor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/152208
Acceso en línea:http://hdl.handle.net/10261/152208
Access Level:acceso abierto
id ES_e2a87dc61bfa20451b92b656af2a678a
oai_identifier_str oai:digital.csic.es:10261/152208
network_acronym_str ES
network_name_str España
repository_id_str
spelling The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid productionAlcaíno, JenniferBravo, NataliaCordova, PamelaMarcoleta, AndrésContreras, GabrielaBarahona, SalvadorSepúlveda, DionisiaFernández Lobato, MaríaBaeza, MarceloCifuentes, VíctorThe red yeast X. dendrorhous is one of the few natural sources of astaxanthin, a carotenoid used in aquaculture for salmonid fish pigmentation and in the cosmetic and pharmaceutical industries for its antioxidant properties. Genetic control of carotenogenesis is well characterized in this yeast; however, little is known about the regulation of the carotenogenesis process. Several lines of evidence have suggested that carotenogenesis is regulated by catabolic repression, and the aim of this work was to identify and functionally characterize the X. dendrorhous MIG1 gene encoding the catabolic repressor Mig1, which mediates transcriptional glucose-dependent repression in other yeasts and fungi. The identified gene encodes a protein of 863 amino acids that demonstrates the characteristic conserved features of Mig1 proteins, and binds in vitro to DNA fragments containing Mig1 boxes. Gene functionality was demonstrated by heterologous complementation in a S. cerevisiae mig1-strain; several aspects of catabolic repression were restored by the X. dendrorhous MIG1 gene. Additionally, a X. dendrorhous mig1- mutant was constructed and demonstrated a higher carotenoid content than the wild-type strain. Most important,the mig1- mutation alleviated the glucose-mediated repression of carotenogenesis in X. dendrorhous: the addition of glucose to mig1- and wild-type cultures promoted the growth of both strains, but carotenoid synthesis was observed only in the mutant strain. Transcriptomic and RT-qPCR analyses revealed that several genes were differentially expressed between X. dendrorhous mig1- and the wild-type strain when cultured with glucose as the sole carbon source. The results obtained in this study demonstrate that catabolic repression in X. dendrorhous is an active process in which the identified MIG1 gene product plays a central role in the regulation of several biological processes, including carotenogenesis.Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1140504 (http://www.conicyt.cl/fondecyt/); Mejoramiento de la Calidad y la Equidad en la Educación Superior (MECESUP) 2-UCH0604 (http://www.mecesup.cl/) NB; and Comisión Nacional de Investigación Científica y Tecnológica (CONICYT)Peer ReviewedPublic Library of ScienceComisión Nacional de Investigación Científica y Tecnológica (Chile)Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/152208reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1522082026-05-22T06:33:51Z
dc.title.none.fl_str_mv The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
title The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
spellingShingle The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
Alcaíno, Jennifer
title_short The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
title_full The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
title_fullStr The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
title_full_unstemmed The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
title_sort The involvement of Mig1 from xanthophyllomyces dendrorhous in catabolic repression: An Active mechanism contributing to the regulation of carotenoid production
dc.creator.none.fl_str_mv Alcaíno, Jennifer
Bravo, Natalia
Cordova, Pamela
Marcoleta, Andrés
Contreras, Gabriela
Barahona, Salvador
Sepúlveda, Dionisia
Fernández Lobato, María
Baeza, Marcelo
Cifuentes, Víctor
author Alcaíno, Jennifer
author_facet Alcaíno, Jennifer
Bravo, Natalia
Cordova, Pamela
Marcoleta, Andrés
Contreras, Gabriela
Barahona, Salvador
Sepúlveda, Dionisia
Fernández Lobato, María
Baeza, Marcelo
Cifuentes, Víctor
author_role author
author2 Bravo, Natalia
Cordova, Pamela
Marcoleta, Andrés
Contreras, Gabriela
Barahona, Salvador
Sepúlveda, Dionisia
Fernández Lobato, María
Baeza, Marcelo
Cifuentes, Víctor
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comisión Nacional de Investigación Científica y Tecnológica (Chile)
Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description The red yeast X. dendrorhous is one of the few natural sources of astaxanthin, a carotenoid used in aquaculture for salmonid fish pigmentation and in the cosmetic and pharmaceutical industries for its antioxidant properties. Genetic control of carotenogenesis is well characterized in this yeast; however, little is known about the regulation of the carotenogenesis process. Several lines of evidence have suggested that carotenogenesis is regulated by catabolic repression, and the aim of this work was to identify and functionally characterize the X. dendrorhous MIG1 gene encoding the catabolic repressor Mig1, which mediates transcriptional glucose-dependent repression in other yeasts and fungi. The identified gene encodes a protein of 863 amino acids that demonstrates the characteristic conserved features of Mig1 proteins, and binds in vitro to DNA fragments containing Mig1 boxes. Gene functionality was demonstrated by heterologous complementation in a S. cerevisiae mig1-strain; several aspects of catabolic repression were restored by the X. dendrorhous MIG1 gene. Additionally, a X. dendrorhous mig1- mutant was constructed and demonstrated a higher carotenoid content than the wild-type strain. Most important,the mig1- mutation alleviated the glucose-mediated repression of carotenogenesis in X. dendrorhous: the addition of glucose to mig1- and wild-type cultures promoted the growth of both strains, but carotenoid synthesis was observed only in the mutant strain. Transcriptomic and RT-qPCR analyses revealed that several genes were differentially expressed between X. dendrorhous mig1- and the wild-type strain when cultured with glucose as the sole carbon source. The results obtained in this study demonstrate that catabolic repression in X. dendrorhous is an active process in which the identified MIG1 gene product plays a central role in the regulation of several biological processes, including carotenogenesis.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/152208
url http://hdl.handle.net/10261/152208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422430566481920
score 15,811543

Similares en LA Referencia