Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing

Porous zirconia scaffolds manufactured using polymer-infiltrated ceramic network (PICN) and additive manufacturing technologies are emerging as promising alternatives to traditional ceramic materials in dental restorations. However, incomplete osseointegration and bacterial infections still represen...

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Autores: García de Albéniz López de Aberasturi, Nerea|||0000-0003-0227-4139, Hodásová, L'udmila|||0000-0002-5113-4610, Buxadera Palomero, Judit|||0000-0003-0897-2093, Jiménez Piqué, Emilio|||0000-0002-6950-611X, Ginebra Molins, Maria Pau|||0000-0002-4700-5621, Llanes Pitarch, Luis Miguel|||0000-0003-1054-1073, Alemán Llansó, Carlos|||0000-0003-4462-6075, Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696, Mas Moruno, Carlos|||0000-0001-8337-0872, Fargas Ribas, Gemma|||0000-0002-5106-1220
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/416210
Acesso em linha:https://hdl.handle.net/2117/416210
https://dx.doi.org/10.1016/j.ceramint.2024.07.088
Access Level:acceso embargado
Palavra-chave:Dental implants
Tissue engineering
Zirconium
Infiltrated zirconia scaffolds
Direct ink writing
Peptide biofunctionalization
Cell adhesion
Antibacterial
Implants dentals
Enginyeria de teixits
Zirconi
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
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spelling Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writingGarcía de Albéniz López de Aberasturi, Nerea|||0000-0003-0227-4139Hodásová, L'udmila|||0000-0002-5113-4610Buxadera Palomero, Judit|||0000-0003-0897-2093Jiménez Piqué, Emilio|||0000-0002-6950-611XGinebra Molins, Maria Pau|||0000-0002-4700-5621Llanes Pitarch, Luis Miguel|||0000-0003-1054-1073Alemán Llansó, Carlos|||0000-0003-4462-6075Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696Mas Moruno, Carlos|||0000-0001-8337-0872Fargas Ribas, Gemma|||0000-0002-5106-1220Dental implantsTissue engineeringZirconiumInfiltrated zirconia scaffoldsDirect ink writingPeptide biofunctionalizationCell adhesionAntibacterialImplants dentalsEnginyeria de teixitsZirconiÀrees temàtiques de la UPC::Enginyeria biomèdica::BiomaterialsPorous zirconia scaffolds manufactured using polymer-infiltrated ceramic network (PICN) and additive manufacturing technologies are emerging as promising alternatives to traditional ceramic materials in dental restorations. However, incomplete osseointegration and bacterial infections still represent challenges for the long-term performance of this new composite material. To address this, the present study aims to investigate the effect of peptide biofunctionalization on the biological performance of infiltrated zirconia scaffold surfaces. The samples used in the work consisted of a 3D-printed zirconia scaffold infiltrated with a dimethacrylate copolymer. Surface biofunctionalization was achieved using a synthetic platform containing the cell-adhesive sequence RGD and the antibacteria LF1-11 peptide (RGD-LF). The attachment of the molecule was characterized through fluorescence confocal laser scanning microscopy and X-ray photoelectron spectroscopy. The biological performance of the samples was evaluated in terms of human mesenchymal stem cell adhesion and early attachment of S. aureus. The physicochemical characterization verified the successful anchoring of the biomolecule to the surface, leading to a peptide density of 288 pmol/cm2. The biological assays confirmed the potential of RGD-LF to improve cell adhesion and spreading. In this sense, the average cell area increased fourfold in the biofunctionalized surface. Regarding bacterial adhesion, it was demonstrated that RGD-LF significantly inhibited it, reducing early adhesion by half compared to the untreated surface. Overall, this study provides valuable insights into the biofunctionalization of polymer-infiltrated 3D scaffolds for the development of cell-instructive and antibacterial surfaces tailored for dental applications.This work has been supported by the project (BASE3D 001-P-001646), which is co-funded by the European Regional Development Fund (ERDF) within the framework of the ERDF Operational Program of Catalonia 2014–2020, and by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant Agreement No. 872869 (RISE Project, Bio-TUNE). The authors also thank financial support from the Spanish Government (Agencia Estatal de Investigación) through grantPeer Reviewed20242024-01-0120242024-10-1820262026-10-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/416210https://dx.doi.org/10.1016/j.ceramint.2024.07.088reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengembargoed accesshttp://purl.org/coar/access_right/c_f1cfinfo:eu-repo/semantics/embargoedAccessoai:upcommons.upc.edu:2117/4162102026-05-27T15:37:01Z
dc.title.none.fl_str_mv Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
title Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
spellingShingle Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
García de Albéniz López de Aberasturi, Nerea|||0000-0003-0227-4139
Dental implants
Tissue engineering
Zirconium
Infiltrated zirconia scaffolds
Direct ink writing
Peptide biofunctionalization
Cell adhesion
Antibacterial
Implants dentals
Enginyeria de teixits
Zirconi
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
title_short Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
title_full Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
title_fullStr Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
title_full_unstemmed Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
title_sort Peptidic biofunctionalization of infiltrated zirconia scaffolds produced by direct ink writing
dc.creator.none.fl_str_mv García de Albéniz López de Aberasturi, Nerea|||0000-0003-0227-4139
Hodásová, L'udmila|||0000-0002-5113-4610
Buxadera Palomero, Judit|||0000-0003-0897-2093
Jiménez Piqué, Emilio|||0000-0002-6950-611X
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Llanes Pitarch, Luis Miguel|||0000-0003-1054-1073
Alemán Llansó, Carlos|||0000-0003-4462-6075
Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696
Mas Moruno, Carlos|||0000-0001-8337-0872
Fargas Ribas, Gemma|||0000-0002-5106-1220
author García de Albéniz López de Aberasturi, Nerea|||0000-0003-0227-4139
author_facet García de Albéniz López de Aberasturi, Nerea|||0000-0003-0227-4139
Hodásová, L'udmila|||0000-0002-5113-4610
Buxadera Palomero, Judit|||0000-0003-0897-2093
Jiménez Piqué, Emilio|||0000-0002-6950-611X
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Llanes Pitarch, Luis Miguel|||0000-0003-1054-1073
Alemán Llansó, Carlos|||0000-0003-4462-6075
Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696
Mas Moruno, Carlos|||0000-0001-8337-0872
Fargas Ribas, Gemma|||0000-0002-5106-1220
author_role author
author2 Hodásová, L'udmila|||0000-0002-5113-4610
Buxadera Palomero, Judit|||0000-0003-0897-2093
Jiménez Piqué, Emilio|||0000-0002-6950-611X
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Llanes Pitarch, Luis Miguel|||0000-0003-1054-1073
Alemán Llansó, Carlos|||0000-0003-4462-6075
Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696
Mas Moruno, Carlos|||0000-0001-8337-0872
Fargas Ribas, Gemma|||0000-0002-5106-1220
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dental implants
Tissue engineering
Zirconium
Infiltrated zirconia scaffolds
Direct ink writing
Peptide biofunctionalization
Cell adhesion
Antibacterial
Implants dentals
Enginyeria de teixits
Zirconi
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
topic Dental implants
Tissue engineering
Zirconium
Infiltrated zirconia scaffolds
Direct ink writing
Peptide biofunctionalization
Cell adhesion
Antibacterial
Implants dentals
Enginyeria de teixits
Zirconi
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
description Porous zirconia scaffolds manufactured using polymer-infiltrated ceramic network (PICN) and additive manufacturing technologies are emerging as promising alternatives to traditional ceramic materials in dental restorations. However, incomplete osseointegration and bacterial infections still represent challenges for the long-term performance of this new composite material. To address this, the present study aims to investigate the effect of peptide biofunctionalization on the biological performance of infiltrated zirconia scaffold surfaces. The samples used in the work consisted of a 3D-printed zirconia scaffold infiltrated with a dimethacrylate copolymer. Surface biofunctionalization was achieved using a synthetic platform containing the cell-adhesive sequence RGD and the antibacteria LF1-11 peptide (RGD-LF). The attachment of the molecule was characterized through fluorescence confocal laser scanning microscopy and X-ray photoelectron spectroscopy. The biological performance of the samples was evaluated in terms of human mesenchymal stem cell adhesion and early attachment of S. aureus. The physicochemical characterization verified the successful anchoring of the biomolecule to the surface, leading to a peptide density of 288 pmol/cm2. The biological assays confirmed the potential of RGD-LF to improve cell adhesion and spreading. In this sense, the average cell area increased fourfold in the biofunctionalized surface. Regarding bacterial adhesion, it was demonstrated that RGD-LF significantly inhibited it, reducing early adhesion by half compared to the untreated surface. Overall, this study provides valuable insights into the biofunctionalization of polymer-infiltrated 3D scaffolds for the development of cell-instructive and antibacterial surfaces tailored for dental applications.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
2024
2024-10-18
2026
2026-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/416210
https://dx.doi.org/10.1016/j.ceramint.2024.07.088
url https://hdl.handle.net/2117/416210
https://dx.doi.org/10.1016/j.ceramint.2024.07.088
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv embargoed access
http://purl.org/coar/access_right/c_f1cf
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv embargoed access
http://purl.org/coar/access_right/c_f1cf
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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