Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug

We have analyzed in a true bug, Graphosoma italicum (Pentatomidae, Hemiptera), the temporal and functional relationships between recombination events, synapsis progression, and SMC1α and SMC3 cohesin axis maturation throughout the male first meiotic prophase. The localization of the histone variant...

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Authors: Viera Vicario, Alberto, Santos, Juan Luis, Parra, Parra Catalán, María Teresa, Calvente, Adela, Gómez Lencero, Rocío, De la Fuente Pita, Roberto, Suja Sánchez, José Ángel, Page Utrilla, Jesús, Rufas, Julio S.
Format: article
Publication Date:2009
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/746601
Online Access:https://hdl.handle.net/10486/746601
https://dx.doi.org/10.1007/s00412-009-0218-4
Access Level:Open access
Keyword:Seminiferous tubule
synaptonemal complex
diffuse stage
sister chromatid cohesion
cohesin complex
Biología y Biomedicina / Biología
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spelling Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bugViera Vicario, AlbertoSantos, Juan LuisParraParra Catalán, María TeresaCalvente, AdelaGómez Lencero, RocíoDe la Fuente Pita, RobertoSuja Sánchez, José ÁngelPage Utrilla, JesúsRufas, Julio S.Seminiferous tubulesynaptonemal complexdiffuse stagesister chromatid cohesioncohesin complexBiología y Biomedicina / BiologíaWe have analyzed in a true bug, Graphosoma italicum (Pentatomidae, Hemiptera), the temporal and functional relationships between recombination events, synapsis progression, and SMC1α and SMC3 cohesin axis maturation throughout the male first meiotic prophase. The localization of the histone variant histone H3 trimethylated at lysine 9 at chromosome ends has allowed us to determine the association of these heterochromatic domains through prophase I stages. Results highlighted that cohesins provide to be good markers for synapsis progression since the formation, morphology, and development of the SMC1α and SMC3 cohesin axes resemble the synaptonemal complex dynamics and, also, that in this species the initiation of recombination precedes synapsis. In addition, we have carried out an accurate cytological characterization of the diffuse stage, which takes place after pachytene, and also analyzed the presence of the cohesin subunits, SMC1α and SMC3, and the recombinase RAD51 at this stage. The mechanisms underlying the absence of SMC1α and SMC3 axes from the diffuse stage onwards are discussedThis work was supported by grants BFU2006-06655, BFU200502431, BFU200800459, and BFU2008-00300-BCM from the Ministerio de Educación y Ciencia SpainSpringerFacultad de CienciasDepartamento de BiologíaAgencia Estatal de Investigación20092009-06-04research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10486/746601https://dx.doi.org/10.1007/s00412-009-0218-419495784reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7466012026-06-23T12:46:27Z
dc.title.none.fl_str_mv Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
title Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
spellingShingle Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
Viera Vicario, Alberto
Seminiferous tubule
synaptonemal complex
diffuse stage
sister chromatid cohesion
cohesin complex
Biología y Biomedicina / Biología
title_short Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
title_full Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
title_fullStr Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
title_full_unstemmed Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
title_sort Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug
dc.creator.none.fl_str_mv Viera Vicario, Alberto
Santos, Juan Luis
Parra
Parra Catalán, María Teresa
Calvente, Adela
Gómez Lencero, Rocío
De la Fuente Pita, Roberto
Suja Sánchez, José Ángel
Page Utrilla, Jesús
Rufas, Julio S.
author Viera Vicario, Alberto
author_facet Viera Vicario, Alberto
Santos, Juan Luis
Parra
Parra Catalán, María Teresa
Calvente, Adela
Gómez Lencero, Rocío
De la Fuente Pita, Roberto
Suja Sánchez, José Ángel
Page Utrilla, Jesús
Rufas, Julio S.
author_role author
author2 Santos, Juan Luis
Parra
Parra Catalán, María Teresa
Calvente, Adela
Gómez Lencero, Rocío
De la Fuente Pita, Roberto
Suja Sánchez, José Ángel
Page Utrilla, Jesús
Rufas, Julio S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias
Departamento de Biología
Agencia Estatal de Investigación
dc.subject.none.fl_str_mv Seminiferous tubule
synaptonemal complex
diffuse stage
sister chromatid cohesion
cohesin complex
Biología y Biomedicina / Biología
topic Seminiferous tubule
synaptonemal complex
diffuse stage
sister chromatid cohesion
cohesin complex
Biología y Biomedicina / Biología
description We have analyzed in a true bug, Graphosoma italicum (Pentatomidae, Hemiptera), the temporal and functional relationships between recombination events, synapsis progression, and SMC1α and SMC3 cohesin axis maturation throughout the male first meiotic prophase. The localization of the histone variant histone H3 trimethylated at lysine 9 at chromosome ends has allowed us to determine the association of these heterochromatic domains through prophase I stages. Results highlighted that cohesins provide to be good markers for synapsis progression since the formation, morphology, and development of the SMC1α and SMC3 cohesin axes resemble the synaptonemal complex dynamics and, also, that in this species the initiation of recombination precedes synapsis. In addition, we have carried out an accurate cytological characterization of the diffuse stage, which takes place after pachytene, and also analyzed the presence of the cohesin subunits, SMC1α and SMC3, and the recombinase RAD51 at this stage. The mechanisms underlying the absence of SMC1α and SMC3 axes from the diffuse stage onwards are discussed
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-06-04
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10486/746601
https://dx.doi.org/10.1007/s00412-009-0218-4
19495784
url https://hdl.handle.net/10486/746601
https://dx.doi.org/10.1007/s00412-009-0218-4
identifier_str_mv 19495784
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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