Wax synthases from sunflower (Helianthus annuus) seeds

Wax esters (WEs) are aliphatic esters of very-long-chain fatty acids and fatty alcohols that contribute to the lipid coatings of many plant organs. In the case of sunflower seeds, these compounds accumulate especially in the seed hull and are synthesized by the action of wax synthase (WS) enzymes th...

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Autores: DeAndrés-Gil, Cristina, Villoslada-Valbuena, Mónica, Venegas-Calerón, Mónica, Moreno-Pérez, Antonio J., Beaudoin, Frédéric, Kurup, Smita, Martínez-Force, Enrique, Garcés Mancheño, Rafael, Salas, Joaquín J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/402799
Acceso en línea:http://hdl.handle.net/10261/402799
https://api.elsevier.com/content/abstract/scopus_id/85218906781
Access Level:acceso abierto
Palabra clave:Cover lipids
Ester synthesis
Sunflower hull
Wax esters
Yeast expression
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spelling Wax synthases from sunflower (Helianthus annuus) seedsDeAndrés-Gil, CristinaVilloslada-Valbuena, MónicaVenegas-Calerón, MónicaMoreno-Pérez, Antonio J.Beaudoin, FrédéricKurup, SmitaMartínez-Force, EnriqueGarcés Mancheño, RafaelSalas, Joaquín J.Cover lipidsEster synthesisSunflower hullWax estersYeast expressionWax esters (WEs) are aliphatic esters of very-long-chain fatty acids and fatty alcohols that contribute to the lipid coatings of many plant organs. In the case of sunflower seeds, these compounds accumulate especially in the seed hull and are synthesized by the action of wax synthase (WS) enzymes through the esterification of acyl-CoA derivatives with very-long-chain fatty alcohols produced by fatty acyl reductases. Here, genes encoding 15 WSs were identified in the sunflower genome, of which 3 are expressed appreciably in the seed during the period of WE accumulation: HaWS6, HaWS8, and HaWS11. The localization and structure of these WSs were studied, and they were expressed in yeast in order to assay the microsome fractions prepared from them. The HaWSs all had very broad specificity profiles for alcohols and they were able to esterify short-chain alcohols (<10 C) with the acyl-CoA supplied. Indeed, simultaneous expression of HaWS8 and HaFAR5 resulted in the production in yeast of some WEs typically present in the sunflower hull.This work was funded by Spanish Ministerio de Ciencia e Innovación through the grants AGL2017 83449R and PID2020–113134RB-I00/MCIN/AEI/10.13039/501100011033, and the CSIC Project I-LINK 2021 Ref. LINKB20078.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)DeAndrés-Gil, Cristina [0000-0003-1496-6562]Villoslada-Valbuena, Mónica [0000-0003-4401-0764]Venegas-Calerón, Mónica [0000-0002-1562-5025]Moreno-Pérez, Antonio J. [0000-0003-4908-8958]Martínez-Force, Enrique [0000-0001-5324-9537]Garcés Mancheño, Rafael [0000-0003-2571-8644]Salas, Joaquín J. [0000-0002-3259-2587]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/402799https://api.elsevier.com/content/abstract/scopus_id/85218906781reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.plaphy.2025.109692https://doi.org/10.1016/j.plaphy.2025.109692Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4027992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Wax synthases from sunflower (Helianthus annuus) seeds
title Wax synthases from sunflower (Helianthus annuus) seeds
spellingShingle Wax synthases from sunflower (Helianthus annuus) seeds
DeAndrés-Gil, Cristina
Cover lipids
Ester synthesis
Sunflower hull
Wax esters
Yeast expression
title_short Wax synthases from sunflower (Helianthus annuus) seeds
title_full Wax synthases from sunflower (Helianthus annuus) seeds
title_fullStr Wax synthases from sunflower (Helianthus annuus) seeds
title_full_unstemmed Wax synthases from sunflower (Helianthus annuus) seeds
title_sort Wax synthases from sunflower (Helianthus annuus) seeds
dc.creator.none.fl_str_mv DeAndrés-Gil, Cristina
Villoslada-Valbuena, Mónica
Venegas-Calerón, Mónica
Moreno-Pérez, Antonio J.
Beaudoin, Frédéric
Kurup, Smita
Martínez-Force, Enrique
Garcés Mancheño, Rafael
Salas, Joaquín J.
author DeAndrés-Gil, Cristina
author_facet DeAndrés-Gil, Cristina
Villoslada-Valbuena, Mónica
Venegas-Calerón, Mónica
Moreno-Pérez, Antonio J.
Beaudoin, Frédéric
Kurup, Smita
Martínez-Force, Enrique
Garcés Mancheño, Rafael
Salas, Joaquín J.
author_role author
author2 Villoslada-Valbuena, Mónica
Venegas-Calerón, Mónica
Moreno-Pérez, Antonio J.
Beaudoin, Frédéric
Kurup, Smita
Martínez-Force, Enrique
Garcés Mancheño, Rafael
Salas, Joaquín J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
DeAndrés-Gil, Cristina [0000-0003-1496-6562]
Villoslada-Valbuena, Mónica [0000-0003-4401-0764]
Venegas-Calerón, Mónica [0000-0002-1562-5025]
Moreno-Pérez, Antonio J. [0000-0003-4908-8958]
Martínez-Force, Enrique [0000-0001-5324-9537]
Garcés Mancheño, Rafael [0000-0003-2571-8644]
Salas, Joaquín J. [0000-0002-3259-2587]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cover lipids
Ester synthesis
Sunflower hull
Wax esters
Yeast expression
topic Cover lipids
Ester synthesis
Sunflower hull
Wax esters
Yeast expression
description Wax esters (WEs) are aliphatic esters of very-long-chain fatty acids and fatty alcohols that contribute to the lipid coatings of many plant organs. In the case of sunflower seeds, these compounds accumulate especially in the seed hull and are synthesized by the action of wax synthase (WS) enzymes through the esterification of acyl-CoA derivatives with very-long-chain fatty alcohols produced by fatty acyl reductases. Here, genes encoding 15 WSs were identified in the sunflower genome, of which 3 are expressed appreciably in the seed during the period of WE accumulation: HaWS6, HaWS8, and HaWS11. The localization and structure of these WSs were studied, and they were expressed in yeast in order to assay the microsome fractions prepared from them. The HaWSs all had very broad specificity profiles for alcohols and they were able to esterify short-chain alcohols (<10 C) with the acyl-CoA supplied. Indeed, simultaneous expression of HaWS8 and HaFAR5 resulted in the production in yeast of some WEs typically present in the sunflower hull.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/402799
https://api.elsevier.com/content/abstract/scopus_id/85218906781
url http://hdl.handle.net/10261/402799
https://api.elsevier.com/content/abstract/scopus_id/85218906781
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.plaphy.2025.109692
https://doi.org/10.1016/j.plaphy.2025.109692
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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