Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells

Magnetosomes are magnetite nanoparticles biosynthesized by magnetotactic bacteria. They have been proposed for several biomedical applications mostly focused on the treatment and diagnosis of cancer. In this work we follow the intracellular long-term fate of magnetosomes in cancer cells and macropha...

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Autores: Gandarias Albaina, Lucia, Gascón Fernández Gubieda, Alicia, Gorni, Giulio, Mathon, Olivier, Olivi, Luca, Abad Díaz de Cerio, Ana, Fernández Gubieda Ruiz, María Luisa, Muela Blázquez, Alicia, García Prieto, Ana
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/63490
Acceso en línea:http://hdl.handle.net/10810/63490
Access Level:acceso abierto
Palabra clave:biomedical applications
magnetic nanoparticle degradation
magnetosomes
magnetotactic bacteria
X-ray absorption spectroscopy
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spelling Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cellsGandarias Albaina, LuciaGascón Fernández Gubieda, AliciaGorni, GiulioMathon, OlivierOlivi, LucaAbad Díaz de Cerio, AnaFernández Gubieda Ruiz, María LuisaMuela Blázquez, AliciaGarcía Prieto, Anabiomedical applicationsmagnetic nanoparticle degradationmagnetosomesmagnetotactic bacteriaX-ray absorption spectroscopyMagnetosomes are magnetite nanoparticles biosynthesized by magnetotactic bacteria. They have been proposed for several biomedical applications mostly focused on the treatment and diagnosis of cancer. In this work we follow the intracellular long-term fate of magnetosomes in cancer cells and macrophages. We observe that cells dispose of magnetosomes using three main mechanisms: splitting into daughter cells in cell division, excretion to the surrounding environment and intracellular degradation. A deeper insight into the later mechanism by means of X-ray absorption spectroscopy reveals that in both cell types studied there is a first oxidation of magnetite to maghemite and a subsequent appearance of ferrihydrite, the iron mineral phase stored in the cores of ferritin proteins, suggesting that cells use the iron released from the degradation of magnetosomes to load ferritin.This work was supported by the grant PID2020-115704RB-C31 funded by MCIN/AEI/10.13039/501100011033 and by the grant IT1479-22 funded by the Basque Government. L.G. would like to thank the financial support from the grant PRE2018-083255 funded by MCIN/AEI/10.13039/501100011033 and by European Union NextGenerationEU/PRTR. G.G. acknowledges financial support from the grant FJC 2020-044866-I funded by MCIN/AEI/10.130339/501100011033 and European Union NextGeneration EU/PRTR.Wiley202320232023info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/63490reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/PID2020-115704RB-C31/info:eu-repo/grantAgreement/MICIU/PRE2018-083255/info:eu-repo/grantAgreement/MICINN/FJC 2020-044866-I/https://onlinelibrary.wiley.com/doi/full/10.1002/biot.202300173info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/es/© 2023 The Authors.Biotechnology Journal published by Wiley-VCHGmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License,which permits use,distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Atribución-NoComercial 3.0 Españaoai:addi.ehu.eus:10810/634902026-06-18T09:23:17Z
dc.title.none.fl_str_mv Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
title Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
spellingShingle Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
Gandarias Albaina, Lucia
biomedical applications
magnetic nanoparticle degradation
magnetosomes
magnetotactic bacteria
X-ray absorption spectroscopy
title_short Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
title_full Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
title_fullStr Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
title_full_unstemmed Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
title_sort Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells
dc.creator.none.fl_str_mv Gandarias Albaina, Lucia
Gascón Fernández Gubieda, Alicia
Gorni, Giulio
Mathon, Olivier
Olivi, Luca
Abad Díaz de Cerio, Ana
Fernández Gubieda Ruiz, María Luisa
Muela Blázquez, Alicia
García Prieto, Ana
author Gandarias Albaina, Lucia
author_facet Gandarias Albaina, Lucia
Gascón Fernández Gubieda, Alicia
Gorni, Giulio
Mathon, Olivier
Olivi, Luca
Abad Díaz de Cerio, Ana
Fernández Gubieda Ruiz, María Luisa
Muela Blázquez, Alicia
García Prieto, Ana
author_role author
author2 Gascón Fernández Gubieda, Alicia
Gorni, Giulio
Mathon, Olivier
Olivi, Luca
Abad Díaz de Cerio, Ana
Fernández Gubieda Ruiz, María Luisa
Muela Blázquez, Alicia
García Prieto, Ana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv biomedical applications
magnetic nanoparticle degradation
magnetosomes
magnetotactic bacteria
X-ray absorption spectroscopy
topic biomedical applications
magnetic nanoparticle degradation
magnetosomes
magnetotactic bacteria
X-ray absorption spectroscopy
description Magnetosomes are magnetite nanoparticles biosynthesized by magnetotactic bacteria. They have been proposed for several biomedical applications mostly focused on the treatment and diagnosis of cancer. In this work we follow the intracellular long-term fate of magnetosomes in cancer cells and macrophages. We observe that cells dispose of magnetosomes using three main mechanisms: splitting into daughter cells in cell division, excretion to the surrounding environment and intracellular degradation. A deeper insight into the later mechanism by means of X-ray absorption spectroscopy reveals that in both cell types studied there is a first oxidation of magnetite to maghemite and a subsequent appearance of ferrihydrite, the iron mineral phase stored in the cores of ferritin proteins, suggesting that cells use the iron released from the degradation of magnetosomes to load ferritin.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/63490
url http://hdl.handle.net/10810/63490
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/PID2020-115704RB-C31/
info:eu-repo/grantAgreement/MICIU/PRE2018-083255/
info:eu-repo/grantAgreement/MICINN/FJC 2020-044866-I/
https://onlinelibrary.wiley.com/doi/full/10.1002/biot.202300173
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/es/
Atribución-NoComercial 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/3.0/es/
Atribución-NoComercial 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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