Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease

Alzheimer’s disease (AD) is a neurodegenerative disorder, characterized by a functional deterioration of the brain. Currently, there are selected biomarkers for its diagnosis in cerebrospinal fluid. However, its extraction has several disadvantages for the patient. Therefore, there is an urgent need...

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Autores: Marrugo-Ramírez, José, Rodríguez-Núñez, Montserrat, Marco, María Pilar, Mir, Mònica, Samitier, Josep
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/226636
Acesso em linha:http://hdl.handle.net/10261/226636
Access Level:acceso abierto
Palavra-chave:Alzheimer’s disease
Immunosensor
Kynurenic acid (KYNA)
Chronoamperometry
Electrochemical biosensor
Electrochemical impedance spectroscopy (EIS)
Blood analysis
Point of care diagnosis (PoC)
In vitro diagnosis (IVD)
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spelling Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s DiseaseMarrugo-Ramírez, JoséRodríguez-Núñez, MontserratMarco, María PilarMir, MònicaSamitier, JosepAlzheimer’s diseaseImmunosensorKynurenic acid (KYNA)ChronoamperometryElectrochemical biosensorElectrochemical impedance spectroscopy (EIS)Blood analysisPoint of care diagnosis (PoC)In vitro diagnosis (IVD)Alzheimer’s disease (AD) is a neurodegenerative disorder, characterized by a functional deterioration of the brain. Currently, there are selected biomarkers for its diagnosis in cerebrospinal fluid. However, its extraction has several disadvantages for the patient. Therefore, there is an urgent need for a detection method using sensitive and selective blood-based biomarkers. Kynurenic acid (KYNA) is a potential biomarker candidate for this purpose. The alteration of the KYNA levels in blood has been related with inflammatory processes in the brain, produced as a protective function when neurons are damaged. This paper describes a novel electrochemical immunosensor for KYNA detection, based on successive functionalization multi-electrode array. The resultant sensor was characterized by cyclic voltammetry (CV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The proposed biosensor detects KYNA within a linear calibration range from 10 pM to 100 nM using CA and EIS, obtaining a limit of detection (LOD) of 16.9 pM and 37.6 pM in buffer, respectively, being the lowest reported LOD for this biomarker. Moreover, to assess our device closer to the real application, the developed immunosensor was also tested under human serum matrix, obtaining an LOD of 391.71 pM for CA and 278.8 pM for EIS with diluted serum.The Nanobioengineering group has support from the Commission for Universities and Research of the Department of Innovation, Universities, and Enterprise of the Generalitat de Catalunya (2017 SGR 1079) and is part of the CERCA Programme/Generalitat de Catalunya. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund (CB06/01/0055). The work developed by the Nanobiotechnology for Diagnostics (Nb4D) group has been funded by the Ministry of Science and Innovation (TryKySeNS project, CTQ2011-29163-C03-01), and the Ministry of Economy and Competitiveness (Molecular links between diabetes and neurodegenerative disorders, Integrated Projects of Excellence in CIBER, PIE14/00061). The Nb4D group is a consolidated research group (Grup de Recerca) of the Generalitat de Catalunya and has support from the Departament d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya (expedient: 2017 SGR 1441).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Marco, María Pilar [0000-0002-2237-7197]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/226636reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CIBER, PIE14info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2011-29163-C03-01https://doi.org/10.3390/bios11010020Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2266362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
title Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
spellingShingle Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
Marrugo-Ramírez, José
Alzheimer’s disease
Immunosensor
Kynurenic acid (KYNA)
Chronoamperometry
Electrochemical biosensor
Electrochemical impedance spectroscopy (EIS)
Blood analysis
Point of care diagnosis (PoC)
In vitro diagnosis (IVD)
title_short Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
title_full Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
title_fullStr Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
title_full_unstemmed Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
title_sort Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease
dc.creator.none.fl_str_mv Marrugo-Ramírez, José
Rodríguez-Núñez, Montserrat
Marco, María Pilar
Mir, Mònica
Samitier, Josep
author Marrugo-Ramírez, José
author_facet Marrugo-Ramírez, José
Rodríguez-Núñez, Montserrat
Marco, María Pilar
Mir, Mònica
Samitier, Josep
author_role author
author2 Rodríguez-Núñez, Montserrat
Marco, María Pilar
Mir, Mònica
Samitier, Josep
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Marco, María Pilar [0000-0002-2237-7197]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Alzheimer’s disease
Immunosensor
Kynurenic acid (KYNA)
Chronoamperometry
Electrochemical biosensor
Electrochemical impedance spectroscopy (EIS)
Blood analysis
Point of care diagnosis (PoC)
In vitro diagnosis (IVD)
topic Alzheimer’s disease
Immunosensor
Kynurenic acid (KYNA)
Chronoamperometry
Electrochemical biosensor
Electrochemical impedance spectroscopy (EIS)
Blood analysis
Point of care diagnosis (PoC)
In vitro diagnosis (IVD)
description Alzheimer’s disease (AD) is a neurodegenerative disorder, characterized by a functional deterioration of the brain. Currently, there are selected biomarkers for its diagnosis in cerebrospinal fluid. However, its extraction has several disadvantages for the patient. Therefore, there is an urgent need for a detection method using sensitive and selective blood-based biomarkers. Kynurenic acid (KYNA) is a potential biomarker candidate for this purpose. The alteration of the KYNA levels in blood has been related with inflammatory processes in the brain, produced as a protective function when neurons are damaged. This paper describes a novel electrochemical immunosensor for KYNA detection, based on successive functionalization multi-electrode array. The resultant sensor was characterized by cyclic voltammetry (CV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The proposed biosensor detects KYNA within a linear calibration range from 10 pM to 100 nM using CA and EIS, obtaining a limit of detection (LOD) of 16.9 pM and 37.6 pM in buffer, respectively, being the lowest reported LOD for this biomarker. Moreover, to assess our device closer to the real application, the developed immunosensor was also tested under human serum matrix, obtaining an LOD of 391.71 pM for CA and 278.8 pM for EIS with diluted serum.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/226636
url http://hdl.handle.net/10261/226636
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CIBER, PIE14
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2011-29163-C03-01
https://doi.org/10.3390/bios11010020
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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