MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer
Background: MicroRNAs (miRNAs) are involved in numerous biological and pathological processes including colorectal cancer (CRC). The aim of our study was to evaluate the ability of miRNA expression patterns to predict chemotherapy response in a cohort of 78 patients with metastatic CRC (mCRC). Metho...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:dnet:idus________::a0afb9fcafba19b32046b6f0f6782cf3 |
| Acceso en línea: | https://hdl.handle.net/11441/187149 https://doi.org/10.1186/1471-2407-14-656 |
| Access Level: | acceso abierto |
| Palabra clave: | MicroRNAs Advanced colorectal cancer Chemotherapy response Prediction |
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MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancerMolina Pinelo, SoniaCarnero, AmancioRivera, FernandoEstevez-Garcia, PurificacionBozada, Juan ManuelLimón, María LuisaPaz-Ares Rodriguez, LuisPortilla de Juan, Fernando de laGarcía Carbonero, RocíoMicroRNAsAdvanced colorectal cancerChemotherapy responsePredictionBackground: MicroRNAs (miRNAs) are involved in numerous biological and pathological processes including colorectal cancer (CRC). The aim of our study was to evaluate the ability of miRNA expression patterns to predict chemotherapy response in a cohort of 78 patients with metastatic CRC (mCRC). Methods: We examined expression levels of 667 miRNAs in the training cohort and evaluated their potential association with relevant clinical endpoints. We identified a miRNA profile that was analysed by RT-qPCR in an independent cohort. For a set of selected miRNAs, bioinformatic target predictions and pathway analysis were also performed. Results: Eight miRNAs (let-7 g*, miR-107, miR-299-5p, miR-337-5p, miR-370, miR-505*, miR-889 and miR-99a-3p) were significant predictors of response to chemotherapy in the training cohort. In addition, overexpression of miR-107, miR-337-5p and miR-99a-3p, and underexpression of miR-889, were also significantly associated with improved progression-free and/or overall survival. MicroRNA-107 and miR-99a-3p were further validated in an independent cohort as predictive markers for chemotherapy response. In addition, an inverse correlation was confirmed in our study population between miR-107 levels and mRNA expression of several potential target genes (CCND1, DICER1, DROSHA and NFKB1). Conclusions: MiR-107 and miR-99a-3p were validated as predictors of response to standard fluoropyrimidine-based chemotherapy in patients with mCRC.BioMed CentralCirugíaFondo de Investigación SanitariaJunta de AndalucíaServicio Andaluz de SaludRTICCFundación Científica de la Asociación Española Contra el CáncerGobierno de EspañaInstituto de Salud Carlos III2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/187149https://doi.org/10.1186/1471-2407-14-656reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBMC Cancer, 14, 656. PI10/02164PI-0259/2007R12/0036/0028CD1100153CD0900148PI12/00137RD12/0036/0028CTS-6844PI-0135-2010PI-0306-2012RD09/0076/00085https://link.springer.com/article/10.1186/1471-2407-14-656info:eu-repo/semantics/openAccessoai:dnet:idus________::a0afb9fcafba19b32046b6f0f6782cf32026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| title |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| spellingShingle |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer Molina Pinelo, Sonia MicroRNAs Advanced colorectal cancer Chemotherapy response Prediction |
| title_short |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| title_full |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| title_fullStr |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| title_full_unstemmed |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| title_sort |
MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer |
| dc.creator.none.fl_str_mv |
Molina Pinelo, Sonia Carnero, Amancio Rivera, Fernando Estevez-Garcia, Purificacion Bozada, Juan Manuel Limón, María Luisa Paz-Ares Rodriguez, Luis Portilla de Juan, Fernando de la García Carbonero, Rocío |
| author |
Molina Pinelo, Sonia |
| author_facet |
Molina Pinelo, Sonia Carnero, Amancio Rivera, Fernando Estevez-Garcia, Purificacion Bozada, Juan Manuel Limón, María Luisa Paz-Ares Rodriguez, Luis Portilla de Juan, Fernando de la García Carbonero, Rocío |
| author_role |
author |
| author2 |
Carnero, Amancio Rivera, Fernando Estevez-Garcia, Purificacion Bozada, Juan Manuel Limón, María Luisa Paz-Ares Rodriguez, Luis Portilla de Juan, Fernando de la García Carbonero, Rocío |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Cirugía Fondo de Investigación Sanitaria Junta de Andalucía Servicio Andaluz de Salud RTICC Fundación Científica de la Asociación Española Contra el Cáncer Gobierno de España Instituto de Salud Carlos III |
| dc.subject.none.fl_str_mv |
MicroRNAs Advanced colorectal cancer Chemotherapy response Prediction |
| topic |
MicroRNAs Advanced colorectal cancer Chemotherapy response Prediction |
| description |
Background: MicroRNAs (miRNAs) are involved in numerous biological and pathological processes including colorectal cancer (CRC). The aim of our study was to evaluate the ability of miRNA expression patterns to predict chemotherapy response in a cohort of 78 patients with metastatic CRC (mCRC). Methods: We examined expression levels of 667 miRNAs in the training cohort and evaluated their potential association with relevant clinical endpoints. We identified a miRNA profile that was analysed by RT-qPCR in an independent cohort. For a set of selected miRNAs, bioinformatic target predictions and pathway analysis were also performed. Results: Eight miRNAs (let-7 g*, miR-107, miR-299-5p, miR-337-5p, miR-370, miR-505*, miR-889 and miR-99a-3p) were significant predictors of response to chemotherapy in the training cohort. In addition, overexpression of miR-107, miR-337-5p and miR-99a-3p, and underexpression of miR-889, were also significantly associated with improved progression-free and/or overall survival. MicroRNA-107 and miR-99a-3p were further validated in an independent cohort as predictive markers for chemotherapy response. In addition, an inverse correlation was confirmed in our study population between miR-107 levels and mRNA expression of several potential target genes (CCND1, DICER1, DROSHA and NFKB1). Conclusions: MiR-107 and miR-99a-3p were validated as predictors of response to standard fluoropyrimidine-based chemotherapy in patients with mCRC. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/187149 https://doi.org/10.1186/1471-2407-14-656 |
| url |
https://hdl.handle.net/11441/187149 https://doi.org/10.1186/1471-2407-14-656 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
BMC Cancer, 14, 656. PI10/02164 PI-0259/2007 R12/0036/0028 CD1100153 CD0900148 PI12/00137 RD12/0036/0028 CTS-6844 PI-0135-2010 PI-0306-2012 RD09/0076/00085 https://link.springer.com/article/10.1186/1471-2407-14-656 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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BioMed Central |
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BioMed Central |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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