Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells

[Introduction]: HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on the viral envelope by the CD169/Siglec-1 membrane receptor. This interaction triggers the internalization of HIV-1 within a structure known as the sac-like compartme...

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Autores: Laguía, Fernando, Chojnacki, Jakub, Erkizia, Itziar, Geli, María Isabel, Enrich, Carlos, Martinez-Picado. Javier, Resa-Infante, Patricia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/379551
Acceso en línea:http://hdl.handle.net/10261/379551
Access Level:acceso abierto
Palabra clave:Dendritic cells
CD169/Siglec1
HIV
Sac-like compartment
MEND
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network_acronym_str ES
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repository_id_str
spelling Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cellsLaguía, FernandoChojnacki, JakubErkizia, ItziarGeli, María IsabelEnrich, CarlosMartinez-Picado. JavierResa-Infante, PatriciaDendritic cellsCD169/Siglec1HIVSac-like compartmentMEND[Introduction]: HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on the viral envelope by the CD169/Siglec-1 membrane receptor. This interaction triggers the internalization of HIV-1 within a structure known as the sac-like compartment. While the mechanism underlying sac-like compartment formation remains elusive, prior research indicates that the process is clathrin-independent and cell membrane cholesterol–dependent and involves transient disruption of cortical actin. Here, we investigate the potential involvement of massive endocytosis (MEND) in this process.[Methods]: We used live cell confocal imaging to measure the dimensions and dynamics of the compartment. We assessed the role of actin and cholesterol in fixed and live cells using confocal microscopy and evaluated the effect of PI3K and protein palmytoilation inhibitors during viral uptake.[Results]: Our data demonstrate extensive plasma membrane invagination based on sac-like compartment dimensions (2.9 μm in diameter and 20 μm3 in volume). We showed that the cholesterol concentration doubles within the regions of viral uptake, suggesting lipid-phase separation, and that development of the sac-like compartment is accompanied by transient depolarization of cortical actin. Moreover, we observed that protein palmitoylation and PI3K inhibition reduce the sac-like compartment formation rate from 70% to 20% and 40%, respectively.[Conclusions]: Our results indicate the involvement of MEND mechanisms during sac-like compartment formation.The authors’ laboratories were supported by funding from the Spanish Ministry of Science and Innovation (grants PID2022-139271OB-I00 and CB21/13/00063, Spain), NIH/NIAID (1UM1AI164561-01 and 1P01AI178376-01, United States of America), Generalitat de València (grant PROMETEO/2021/036, Spain), and Grifols (Spain).Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)National Institutes of Health (US)National Institute of Allergy and Infectious Diseases (US)Generalitat ValencianaGrifolsConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/379551reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-139271OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3389/fimmu.2024.1505840https://doi.org/10.3389/fimmu.2024.1505840Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3795512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
title Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
spellingShingle Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
Laguía, Fernando
Dendritic cells
CD169/Siglec1
HIV
Sac-like compartment
MEND
title_short Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
title_full Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
title_fullStr Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
title_full_unstemmed Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
title_sort Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells
dc.creator.none.fl_str_mv Laguía, Fernando
Chojnacki, Jakub
Erkizia, Itziar
Geli, María Isabel
Enrich, Carlos
Martinez-Picado. Javier
Resa-Infante, Patricia
author Laguía, Fernando
author_facet Laguía, Fernando
Chojnacki, Jakub
Erkizia, Itziar
Geli, María Isabel
Enrich, Carlos
Martinez-Picado. Javier
Resa-Infante, Patricia
author_role author
author2 Chojnacki, Jakub
Erkizia, Itziar
Geli, María Isabel
Enrich, Carlos
Martinez-Picado. Javier
Resa-Infante, Patricia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
National Institutes of Health (US)
National Institute of Allergy and Infectious Diseases (US)
Generalitat Valenciana
Grifols
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Dendritic cells
CD169/Siglec1
HIV
Sac-like compartment
MEND
topic Dendritic cells
CD169/Siglec1
HIV
Sac-like compartment
MEND
description [Introduction]: HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on the viral envelope by the CD169/Siglec-1 membrane receptor. This interaction triggers the internalization of HIV-1 within a structure known as the sac-like compartment. While the mechanism underlying sac-like compartment formation remains elusive, prior research indicates that the process is clathrin-independent and cell membrane cholesterol–dependent and involves transient disruption of cortical actin. Here, we investigate the potential involvement of massive endocytosis (MEND) in this process.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/379551
url http://hdl.handle.net/10261/379551
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-139271OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3389/fimmu.2024.1505840
https://doi.org/10.3389/fimmu.2024.1505840
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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