Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles

Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organi...

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Autores: Srinivasan, Sumithra Yasaswini, Álvarez Illera, Pilar, Kukhtar, Dmytro, Benseny Cases, Núria, Cerón Madrigal, Julián, Álvarez, Javier, Fonteriz, Rosalba I., Montero, Mayte, Laromaine, Anna
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/214026
Acesso em linha:https://hdl.handle.net/2445/214026
Access Level:Acceso aberto
Palavra-chave:Arrítmia
Nanomedicina
Arrhythmia
Nanomedicine
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spelling Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole NanoparticlesSrinivasan, Sumithra YasaswiniÁlvarez Illera, PilarKukhtar, DmytroBenseny Cases, NúriaCerón Madrigal, JuliánÁlvarez, JavierFonteriz, Rosalba I.Montero, MayteLaromaine, AnnaArrítmiaNanomedicinaArrhythmiaNanomedicineExperimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes' pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs' biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs.American Chemical Society (ACS)2024202420232024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/214026Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1021/acsnano.3c05245ACS Nano, 2023, vol. 17, num. 17, p. 17273-17284https://doi.org/10.1021/acsnano.3c05245cc by (c) Srinivasan, Sumithra Yasaswini et al, 2024http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2140262026-05-29T05:05:01Z
dc.title.none.fl_str_mv Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
title Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
spellingShingle Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
Srinivasan, Sumithra Yasaswini
Arrítmia
Nanomedicina
Arrhythmia
Nanomedicine
title_short Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
title_full Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
title_fullStr Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
title_full_unstemmed Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
title_sort Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles
dc.creator.none.fl_str_mv Srinivasan, Sumithra Yasaswini
Álvarez Illera, Pilar
Kukhtar, Dmytro
Benseny Cases, Núria
Cerón Madrigal, Julián
Álvarez, Javier
Fonteriz, Rosalba I.
Montero, Mayte
Laromaine, Anna
author Srinivasan, Sumithra Yasaswini
author_facet Srinivasan, Sumithra Yasaswini
Álvarez Illera, Pilar
Kukhtar, Dmytro
Benseny Cases, Núria
Cerón Madrigal, Julián
Álvarez, Javier
Fonteriz, Rosalba I.
Montero, Mayte
Laromaine, Anna
author_role author
author2 Álvarez Illera, Pilar
Kukhtar, Dmytro
Benseny Cases, Núria
Cerón Madrigal, Julián
Álvarez, Javier
Fonteriz, Rosalba I.
Montero, Mayte
Laromaine, Anna
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arrítmia
Nanomedicina
Arrhythmia
Nanomedicine
topic Arrítmia
Nanomedicina
Arrhythmia
Nanomedicine
description Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes' pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs' biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/214026
url https://hdl.handle.net/2445/214026
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1021/acsnano.3c05245
ACS Nano, 2023, vol. 17, num. 17, p. 17273-17284
https://doi.org/10.1021/acsnano.3c05245
dc.rights.none.fl_str_mv cc by (c) Srinivasan, Sumithra Yasaswini et al, 2024
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Srinivasan, Sumithra Yasaswini et al, 2024
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society (ACS)
publisher.none.fl_str_mv American Chemical Society (ACS)
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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