Bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria

[Background] Bacteroides fragilis is the most frequent species at the human intestinal mucosal surface, it contributes to the maturation of the immune system although is also considered as an opportunistic pathogen. Some Bifidobacterium strains produce exopolysaccharides (EPS), complex carbohydrate...

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Detalhes bibliográficos
Autores: Rios-Covián, David, Cuesta Suárez, Isabel, Álvarez-Buylla, Jorge R., Ruas-Madiedo, Patricia, Gueimonde Fernández, Miguel, González de los Reyes-Gavilán, Clara
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/143259
Acesso em linha:http://hdl.handle.net/10261/143259
Access Level:acceso abierto
Palavra-chave:Heteropolysaccharides
MALLS
Bifidobacterium
Bacteroides fragilis
Exopolysaccharides
Descrição
Resumo:[Background] Bacteroides fragilis is the most frequent species at the human intestinal mucosal surface, it contributes to the maturation of the immune system although is also considered as an opportunistic pathogen. Some Bifidobacterium strains produce exopolysaccharides (EPS), complex carbohydrate polymers that promote changes in the metabolism of B. fragilis when this microorganism grows in their presence. To demonstrate that B. fragilis can use EPS from bifidobacteria as fermentable substrates, purified EPS fractions from two strains, Bifidobacterium longum E44 and Bifidobacterium animalis subsp. lactis R1, were added as the sole carbon source in cultures of B. fragilis DSMZ 2151 in a minimal medium. Bacterial counts were determined during incubation and the evolution of organic acids, short chain fatty acids (SCFA) and evolution of EPS fractions was analysed by chromatography.