Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system

The biofilm formation capacity of Staphylococcus aureus clinical isolates is considered an important virulence factor for the establishment of chronic infections. Environmental conditions affect the biofilm formation capacity of S. aureus, indicating the existence of positive and negative regulators...

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Authors: Toledo Arana, Alejandro, Merino Barberá, Nekane, Vergara Irigaray, Marta, Débarbouillé, Michel, Penadés, José R., Lasa Uzcudun, Íñigo
Format: article
Status:Published version
Publication Date:2005
Country:España
Institution:Universidad Pública de Navarra
Repository:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/32028
Online Access:https://hdl.handle.net/2454/32028
Access Level:Open access
Keyword:Staphylococcus aureus
arlRS
Biofilm development
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spelling Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component systemToledo Arana, AlejandroMerino Barberá, NekaneVergara Irigaray, MartaDébarbouillé, MichelPenadés, José R.Lasa Uzcudun, ÍñigoStaphylococcus aureusarlRSBiofilm developmentThe biofilm formation capacity of Staphylococcus aureus clinical isolates is considered an important virulence factor for the establishment of chronic infections. Environmental conditions affect the biofilm formation capacity of S. aureus, indicating the existence of positive and negative regulators of the process. The majority of the screening procedures for identifying genes involved in biofilm development have been focused on genes whose presence is essential for the process. In this report, we have used random transposon mutagenesis and systematic disruption of all S. aureus two-component systems to identify negative regulators of S. aureus biofilm development in a chemically defined medium (Hussain-Hastings-White modified medium [HHWm]). The results of both approaches coincided in that they identified arlRS as a repressor of biofilm development under both steady-state and flow conditions. The arlRS mutant exhibited an increased initial attachment as well as increased accumulation of poly-N-acetylglucosamine (PNAG). However, the biofilm formation of the arlRS mutant was not affected when the icaADBC operon was deleted, indicating that PNAG is not an essential compound of the biofilm matrix produced in HHWm. Disruption of the major autolysin gene, atl, did not produce any effect on the biofilm phenotype of an arlRS mutant. Epistatic experiments with global regulators involved in staphylococcal-biofilm formation indicated that sarA deletion abolished, whereas agr deletion reinforced, the biofilm development promoted by the arlRS mutation.This work was supported by the BIO2002-04542-C02 and GEN2003-20234-C06-05 grants from the Comisión Interministerial de Ciencia y Tecnología and grant 17/2004 from the Departamento de Educación y Cultura del Gobierno de Navarra, Spain.American Society for MicrobiologyIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako InstitutuaGobierno de Navarra / Nafarroako Gobernua2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/32028reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2005, American Society for Microbiology. All Rights Reserved.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/320282026-06-17T12:41:47Z
dc.title.none.fl_str_mv Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
title Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
spellingShingle Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
Toledo Arana, Alejandro
Staphylococcus aureus
arlRS
Biofilm development
title_short Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
title_full Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
title_fullStr Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
title_full_unstemmed Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
title_sort Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
dc.creator.none.fl_str_mv Toledo Arana, Alejandro
Merino Barberá, Nekane
Vergara Irigaray, Marta
Débarbouillé, Michel
Penadés, José R.
Lasa Uzcudun, Íñigo
author Toledo Arana, Alejandro
author_facet Toledo Arana, Alejandro
Merino Barberá, Nekane
Vergara Irigaray, Marta
Débarbouillé, Michel
Penadés, José R.
Lasa Uzcudun, Íñigo
author_role author
author2 Merino Barberá, Nekane
Vergara Irigaray, Marta
Débarbouillé, Michel
Penadés, José R.
Lasa Uzcudun, Íñigo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Gobierno de Navarra / Nafarroako Gobernua
dc.subject.none.fl_str_mv Staphylococcus aureus
arlRS
Biofilm development
topic Staphylococcus aureus
arlRS
Biofilm development
description The biofilm formation capacity of Staphylococcus aureus clinical isolates is considered an important virulence factor for the establishment of chronic infections. Environmental conditions affect the biofilm formation capacity of S. aureus, indicating the existence of positive and negative regulators of the process. The majority of the screening procedures for identifying genes involved in biofilm development have been focused on genes whose presence is essential for the process. In this report, we have used random transposon mutagenesis and systematic disruption of all S. aureus two-component systems to identify negative regulators of S. aureus biofilm development in a chemically defined medium (Hussain-Hastings-White modified medium [HHWm]). The results of both approaches coincided in that they identified arlRS as a repressor of biofilm development under both steady-state and flow conditions. The arlRS mutant exhibited an increased initial attachment as well as increased accumulation of poly-N-acetylglucosamine (PNAG). However, the biofilm formation of the arlRS mutant was not affected when the icaADBC operon was deleted, indicating that PNAG is not an essential compound of the biofilm matrix produced in HHWm. Disruption of the major autolysin gene, atl, did not produce any effect on the biofilm phenotype of an arlRS mutant. Epistatic experiments with global regulators involved in staphylococcal-biofilm formation indicated that sarA deletion abolished, whereas agr deletion reinforced, the biofilm development promoted by the arlRS mutation.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/32028
url https://hdl.handle.net/2454/32028
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2005, American Society for Microbiology. All Rights Reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2005, American Society for Microbiology. All Rights Reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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