PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges

Santos, J.L. (José Luis)|||/items/b14e2212-42c2-440a-a236-430df8446638; Krause, B.J. (Bernardo J.)|||/items/3a749511-3128-4b1c-9c53-61cde7e7c3a4; Cataldo, L.R. (Luis R.)|||/items/2f41b90a-4277-402e-9da2-71d8c7194ba0; Vega, J.A. (Javier Andrés)|||/items/63765799-5090-4d57-93ae-d240f4fd50a8; Salas-Pérez, F. (Francisca)|||/items/60f268d1-df7d-40f1-8880-272903343a37; Mennickent, P. (Paula)|||/items/19a07304-6787-4aa6-8c85-1d40ac2bfa32; Gallegos, R. (Raúl)|||/items/62e367e5-63e1-4063-847b-cb1dddc94c31; Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb; Prieto-Hontoria, P.L. (Pedro L.)|||/items/74b9fcba-d3ed-4a1c-8979-86b8359cb6c7; Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6; Bravo, C. (Carolina)|||/items/3243a990-7625-4666-b20a-efd2a2be95e8; Salas-Huetos, A. (Albert)|||/items/f6375d33-94e9-4ca9-93be-6010e7adb674; Arpon, A. (Ana)|||/items/e1ea7066-dbdf-42e3-b6a9-b9f5c86adc2f; Galgani, J.E. (José E.)|||/items/547e4962-3a7f-4d6d-ac5c-c93440270723; Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131

PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges

Methylation in CpG sites of the PPARGC1A gene (encoding PGC1-α) has been associated with adiposity, insulin secretion/sensitivity indexes and type 2 diabetes. We assessed the association between the methylation profile of the PPARGC1A gene promoter gene in leukocytes with insulin secretion/sensitivi...

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Autores:	Santos, J.L. (José Luis)\|\|\|/items/b14e2212-42c2-440a-a236-430df8446638, Krause, B.J. (Bernardo J.)\|\|\|/items/3a749511-3128-4b1c-9c53-61cde7e7c3a4, Cataldo, L.R. (Luis R.)\|\|\|/items/2f41b90a-4277-402e-9da2-71d8c7194ba0, Vega, J.A. (Javier Andrés)\|\|\|/items/63765799-5090-4d57-93ae-d240f4fd50a8, Salas-Pérez, F. (Francisca)\|\|\|/items/60f268d1-df7d-40f1-8880-272903343a37, Mennickent, P. (Paula)\|\|\|/items/19a07304-6787-4aa6-8c85-1d40ac2bfa32, Gallegos, R. (Raúl)\|\|\|/items/62e367e5-63e1-4063-847b-cb1dddc94c31, Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Prieto-Hontoria, P.L. (Pedro L.)\|\|\|/items/74b9fcba-d3ed-4a1c-8979-86b8359cb6c7, Riezu-Boj, J.I. (José Ignacio)\|\|\|/items/87349e89-7360-4cdd-81be-2e8a6174b5f6, Bravo, C. (Carolina)\|\|\|/items/3243a990-7625-4666-b20a-efd2a2be95e8, Salas-Huetos, A. (Albert)\|\|\|/items/f6375d33-94e9-4ca9-93be-6010e7adb674, Arpon, A. (Ana)\|\|\|/items/e1ea7066-dbdf-42e3-b6a9-b9f5c86adc2f, Galgani, J.E. (José E.)\|\|\|/items/547e4962-3a7f-4d6d-ac5c-c93440270723, Martinez, J.A. (José Alfredo)\|\|\|/items/6a3581ea-897b-4439-a95c-19301775e131
Tipo de recurso:	artículo
Fecha de publicación:	2020
País:	España
Institución:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/67698
Acceso en línea:	https://hdl.handle.net/10171/67698
Access Level:	acceso abierto
Palabra clave:	Methylation Insulin sensitivity Insulin secretion Biomarker Gene variant PPARGC1A gene

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spelling	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challengesSantos, J.L. (José Luis)\|\|\|/items/b14e2212-42c2-440a-a236-430df8446638Krause, B.J. (Bernardo J.)\|\|\|/items/3a749511-3128-4b1c-9c53-61cde7e7c3a4Cataldo, L.R. (Luis R.)\|\|\|/items/2f41b90a-4277-402e-9da2-71d8c7194ba0Vega, J.A. (Javier Andrés)\|\|\|/items/63765799-5090-4d57-93ae-d240f4fd50a8Salas-Pérez, F. (Francisca)\|\|\|/items/60f268d1-df7d-40f1-8880-272903343a37Mennickent, P. (Paula)\|\|\|/items/19a07304-6787-4aa6-8c85-1d40ac2bfa32Gallegos, R. (Raúl)\|\|\|/items/62e367e5-63e1-4063-847b-cb1dddc94c31Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebPrieto-Hontoria, P.L. (Pedro L.)\|\|\|/items/74b9fcba-d3ed-4a1c-8979-86b8359cb6c7Riezu-Boj, J.I. (José Ignacio)\|\|\|/items/87349e89-7360-4cdd-81be-2e8a6174b5f6Bravo, C. (Carolina)\|\|\|/items/3243a990-7625-4666-b20a-efd2a2be95e8Salas-Huetos, A. (Albert)\|\|\|/items/f6375d33-94e9-4ca9-93be-6010e7adb674Arpon, A. (Ana)\|\|\|/items/e1ea7066-dbdf-42e3-b6a9-b9f5c86adc2fGalgani, J.E. (José E.)\|\|\|/items/547e4962-3a7f-4d6d-ac5c-c93440270723Martinez, J.A. (José Alfredo)\|\|\|/items/6a3581ea-897b-4439-a95c-19301775e131MethylationInsulin sensitivityInsulin secretionBiomarkerGene variantPPARGC1A geneMethylation in CpG sites of the PPARGC1A gene (encoding PGC1-α) has been associated with adiposity, insulin secretion/sensitivity indexes and type 2 diabetes. We assessed the association between the methylation profile of the PPARGC1A gene promoter gene in leukocytes with insulin secretion/sensitivity indexes in normoglycemic women. A standard oral glucose tolerance test (OGTT) and an abbreviated version of the intravenous glucose tolerance test (IVGTT) were carried out in n = 57 Chilean nondiabetic women with measurements of plasma glucose, insulin, and C-peptide. Bisulfite-treated DNA from leukocytes was evaluated for methylation levels in six CpG sites of the proximal promoter of the PPARGC1A gene by pyrosequencing (positions -816, -783, -652, -617, -521 and -515). A strong correlation between the DNA methylation percentage of different CpG sites of the PPARGC1A promoter in leukocytes was found, suggesting an integrated epigenetic control of this region. We found a positive association between the methylation levels of the CpG site -783 with the insulin sensitivity Matsuda composite index (rho = 0.31; p = 0.02) derived from the OGTT. The CpG hypomethylation in the promoter position -783 of the PPARGC1A gene in leukocytes may represent a biomarker of reduced insulin sensitivity after the ingestion of glucose.MDPI AGDadun. Depósito Académico Digital Universidad de Navarra20232023-10-2320202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/67698reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/676982026-06-21T12:47:57Z
dc.title.none.fl_str_mv	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
title	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
spellingShingle	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges Santos, J.L. (José Luis)\|\|\|/items/b14e2212-42c2-440a-a236-430df8446638 Methylation Insulin sensitivity Insulin secretion Biomarker Gene variant PPARGC1A gene
title_short	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
title_full	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
title_fullStr	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
title_full_unstemmed	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
title_sort	PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges
dc.creator.none.fl_str_mv	Santos, J.L. (José Luis)\|\|\|/items/b14e2212-42c2-440a-a236-430df8446638 Krause, B.J. (Bernardo J.)\|\|\|/items/3a749511-3128-4b1c-9c53-61cde7e7c3a4 Cataldo, L.R. (Luis R.)\|\|\|/items/2f41b90a-4277-402e-9da2-71d8c7194ba0 Vega, J.A. (Javier Andrés)\|\|\|/items/63765799-5090-4d57-93ae-d240f4fd50a8 Salas-Pérez, F. (Francisca)\|\|\|/items/60f268d1-df7d-40f1-8880-272903343a37 Mennickent, P. (Paula)\|\|\|/items/19a07304-6787-4aa6-8c85-1d40ac2bfa32 Gallegos, R. (Raúl)\|\|\|/items/62e367e5-63e1-4063-847b-cb1dddc94c31 Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Prieto-Hontoria, P.L. (Pedro L.)\|\|\|/items/74b9fcba-d3ed-4a1c-8979-86b8359cb6c7 Riezu-Boj, J.I. (José Ignacio)\|\|\|/items/87349e89-7360-4cdd-81be-2e8a6174b5f6 Bravo, C. (Carolina)\|\|\|/items/3243a990-7625-4666-b20a-efd2a2be95e8 Salas-Huetos, A. (Albert)\|\|\|/items/f6375d33-94e9-4ca9-93be-6010e7adb674 Arpon, A. (Ana)\|\|\|/items/e1ea7066-dbdf-42e3-b6a9-b9f5c86adc2f Galgani, J.E. (José E.)\|\|\|/items/547e4962-3a7f-4d6d-ac5c-c93440270723 Martinez, J.A. (José Alfredo)\|\|\|/items/6a3581ea-897b-4439-a95c-19301775e131
author	Santos, J.L. (José Luis)\|\|\|/items/b14e2212-42c2-440a-a236-430df8446638
author_facet	Santos, J.L. (José Luis)\|\|\|/items/b14e2212-42c2-440a-a236-430df8446638 Krause, B.J. (Bernardo J.)\|\|\|/items/3a749511-3128-4b1c-9c53-61cde7e7c3a4 Cataldo, L.R. (Luis R.)\|\|\|/items/2f41b90a-4277-402e-9da2-71d8c7194ba0 Vega, J.A. (Javier Andrés)\|\|\|/items/63765799-5090-4d57-93ae-d240f4fd50a8 Salas-Pérez, F. (Francisca)\|\|\|/items/60f268d1-df7d-40f1-8880-272903343a37 Mennickent, P. (Paula)\|\|\|/items/19a07304-6787-4aa6-8c85-1d40ac2bfa32 Gallegos, R. (Raúl)\|\|\|/items/62e367e5-63e1-4063-847b-cb1dddc94c31 Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Prieto-Hontoria, P.L. (Pedro L.)\|\|\|/items/74b9fcba-d3ed-4a1c-8979-86b8359cb6c7 Riezu-Boj, J.I. (José Ignacio)\|\|\|/items/87349e89-7360-4cdd-81be-2e8a6174b5f6 Bravo, C. (Carolina)\|\|\|/items/3243a990-7625-4666-b20a-efd2a2be95e8 Salas-Huetos, A. (Albert)\|\|\|/items/f6375d33-94e9-4ca9-93be-6010e7adb674 Arpon, A. (Ana)\|\|\|/items/e1ea7066-dbdf-42e3-b6a9-b9f5c86adc2f Galgani, J.E. (José E.)\|\|\|/items/547e4962-3a7f-4d6d-ac5c-c93440270723 Martinez, J.A. (José Alfredo)\|\|\|/items/6a3581ea-897b-4439-a95c-19301775e131
author_role	author
author2	Krause, B.J. (Bernardo J.)\|\|\|/items/3a749511-3128-4b1c-9c53-61cde7e7c3a4 Cataldo, L.R. (Luis R.)\|\|\|/items/2f41b90a-4277-402e-9da2-71d8c7194ba0 Vega, J.A. (Javier Andrés)\|\|\|/items/63765799-5090-4d57-93ae-d240f4fd50a8 Salas-Pérez, F. (Francisca)\|\|\|/items/60f268d1-df7d-40f1-8880-272903343a37 Mennickent, P. (Paula)\|\|\|/items/19a07304-6787-4aa6-8c85-1d40ac2bfa32 Gallegos, R. (Raúl)\|\|\|/items/62e367e5-63e1-4063-847b-cb1dddc94c31 Milagro-Yoldi, F.I. (Fermín Ignacio)\|\|\|/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb Prieto-Hontoria, P.L. (Pedro L.)\|\|\|/items/74b9fcba-d3ed-4a1c-8979-86b8359cb6c7 Riezu-Boj, J.I. (José Ignacio)\|\|\|/items/87349e89-7360-4cdd-81be-2e8a6174b5f6 Bravo, C. (Carolina)\|\|\|/items/3243a990-7625-4666-b20a-efd2a2be95e8 Salas-Huetos, A. (Albert)\|\|\|/items/f6375d33-94e9-4ca9-93be-6010e7adb674 Arpon, A. (Ana)\|\|\|/items/e1ea7066-dbdf-42e3-b6a9-b9f5c86adc2f Galgani, J.E. (José E.)\|\|\|/items/547e4962-3a7f-4d6d-ac5c-c93440270723 Martinez, J.A. (José Alfredo)\|\|\|/items/6a3581ea-897b-4439-a95c-19301775e131
author2_role	author author author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Methylation Insulin sensitivity Insulin secretion Biomarker Gene variant PPARGC1A gene
topic	Methylation Insulin sensitivity Insulin secretion Biomarker Gene variant PPARGC1A gene
description	Methylation in CpG sites of the PPARGC1A gene (encoding PGC1-α) has been associated with adiposity, insulin secretion/sensitivity indexes and type 2 diabetes. We assessed the association between the methylation profile of the PPARGC1A gene promoter gene in leukocytes with insulin secretion/sensitivity indexes in normoglycemic women. A standard oral glucose tolerance test (OGTT) and an abbreviated version of the intravenous glucose tolerance test (IVGTT) were carried out in n = 57 Chilean nondiabetic women with measurements of plasma glucose, insulin, and C-peptide. Bisulfite-treated DNA from leukocytes was evaluated for methylation levels in six CpG sites of the proximal promoter of the PPARGC1A gene by pyrosequencing (positions -816, -783, -652, -617, -521 and -515). A strong correlation between the DNA methylation percentage of different CpG sites of the PPARGC1A promoter in leukocytes was found, suggesting an integrated epigenetic control of this region. We found a positive association between the methylation levels of the CpG site -783 with the insulin sensitivity Matsuda composite index (rho = 0.31; p = 0.02) derived from the OGTT. The CpG hypomethylation in the promoter position -783 of the PPARGC1A gene in leukocytes may represent a biomarker of reduced insulin sensitivity after the ingestion of glucose.
publishDate	2020
dc.date.none.fl_str_mv	2020 2020-01-01 2020 2020-01-01 2023 2023-10-23
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/67698
url	https://hdl.handle.net/10171/67698
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI AG
publisher.none.fl_str_mv	MDPI AG
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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PPARGC1A gene promoter methylation as a biomarker of insulin secretion and sensitivity in response to glucose challenges

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