Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology

Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked w...

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Authors: Van Hoeck, V., Sturmey, Roger, Bermejo Álvarez, Pablo, Rizos, Dimitrios, Gutiérrez-Adán, Alfonso, Leese, H. J., Bols, P. E. J., Leroy, J. L. M. R.
Format: article
Publication Date:2011
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/290701
Online Access:http://hdl.handle.net/10261/290701
Access Level:Open access
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spelling Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiologyVan Hoeck, V.Sturmey, RogerBermejo Álvarez, PabloRizos, DimitriosGutiérrez-Adán, AlfonsoLeese, H. J.Bols, P. E. J.Leroy, J. L. M. R.Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked whether elevated NEFA concentrations during oocyte maturation affect the development and physiology of zygotes formed from such oocytes, using the cow as a model. The zygotes were grown to blastocysts, which were evaluated for their quality in terms of cell number, apoptosis, expression of key genes, amino acid turnover and oxidative metabolism. Oocyte maturation under elevated NEFA concentrations resulted in blastocysts with significantly lower cell number, increased apoptotic cell ratio and altered mRNA abundance of DNMT3A, IGF2R and SLC2A1. In addition, the blastocysts displayed reduced oxygen, pyruvate and glucose consumption, up-regulated lactate consumption and higher amino acid metabolism. These data indicate that exposure of maturing oocytes to elevated NEFA concentrations has a negative impact on fertility not only through a reduction in oocyte developmental capacity but through compromised early embryo quality, viability and metabolism. © 2011 Van Hoeck et al.Peer reviewedPublic Library of ScienceRizos, Dimitrios [0000-0001-6813-3940]Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]Bermejo Álvarez, Pablo [0000-0001-9907-2626]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/290701reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2907012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
title Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
spellingShingle Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
Van Hoeck, V.
title_short Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
title_full Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
title_fullStr Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
title_full_unstemmed Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
title_sort Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology
dc.creator.none.fl_str_mv Van Hoeck, V.
Sturmey, Roger
Bermejo Álvarez, Pablo
Rizos, Dimitrios
Gutiérrez-Adán, Alfonso
Leese, H. J.
Bols, P. E. J.
Leroy, J. L. M. R.
author Van Hoeck, V.
author_facet Van Hoeck, V.
Sturmey, Roger
Bermejo Álvarez, Pablo
Rizos, Dimitrios
Gutiérrez-Adán, Alfonso
Leese, H. J.
Bols, P. E. J.
Leroy, J. L. M. R.
author_role author
author2 Sturmey, Roger
Bermejo Álvarez, Pablo
Rizos, Dimitrios
Gutiérrez-Adán, Alfonso
Leese, H. J.
Bols, P. E. J.
Leroy, J. L. M. R.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Rizos, Dimitrios [0000-0001-6813-3940]
Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]
Bermejo Álvarez, Pablo [0000-0001-9907-2626]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked whether elevated NEFA concentrations during oocyte maturation affect the development and physiology of zygotes formed from such oocytes, using the cow as a model. The zygotes were grown to blastocysts, which were evaluated for their quality in terms of cell number, apoptosis, expression of key genes, amino acid turnover and oxidative metabolism. Oocyte maturation under elevated NEFA concentrations resulted in blastocysts with significantly lower cell number, increased apoptotic cell ratio and altered mRNA abundance of DNMT3A, IGF2R and SLC2A1. In addition, the blastocysts displayed reduced oxygen, pyruvate and glucose consumption, up-regulated lactate consumption and higher amino acid metabolism. These data indicate that exposure of maturing oocytes to elevated NEFA concentrations has a negative impact on fertility not only through a reduction in oocyte developmental capacity but through compromised early embryo quality, viability and metabolism. © 2011 Van Hoeck et al.
publishDate 2011
dc.date.none.fl_str_mv 2011
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/290701
url http://hdl.handle.net/10261/290701
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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