Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses
The interface tissue between bone and soft tissues, such as tendon and ligament (TL), is highly prone to injury. Although different biomaterials have been developed for TL regeneration, few address the challenges of the TL bone interface. Here, we aim to develop novel hybrid nanocomposites based on...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/68134 |
| Acceso en línea: | https://hdl.handle.net/10171/68134 |
| Access Level: | acceso abierto |
| Palabra clave: | Silane modification Tendon enthesis Hydroxyapatite Nanocomposites Interface tissue |
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Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responsesHeidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbbMuiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58eRuan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11eeVahabli, E. (Ebrahim)|||/items/170429ea-8710-4c77-9011-d486b9d16341Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1aJuan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3Silane modificationTendon enthesisHydroxyapatiteNanocompositesInterface tissueThe interface tissue between bone and soft tissues, such as tendon and ligament (TL), is highly prone to injury. Although different biomaterials have been developed for TL regeneration, few address the challenges of the TL bone interface. Here, we aim to develop novel hybrid nanocomposites based on poly(p-dioxanone) (PDO), poly (lactide-co-caprolactone) (LCL), and hydroxyapatite (HA) nanoparticles suitable for TL-bone interface repair. Nanocomposites, containing 3-10% of both unmodified and chemically modified hydroxyapatite (mHA) with a silane coupling agent. We then explored biocompatibility through in vitro and in vivo studies using a subcutaneous mouse model. Through different characterisation tests, we found that mHA increases tensile properties, creates rougher surfaces, and reduces crystallinity and hydrophilicity. Morphological observations indicate that mHA nanoparticles are attracted by PDO rather than LCL phase, resulting in a higher degradation rate for mHA group. We found that adding the 5% of nanoparticles gives a balance between the properties. In vitro experiments show that osteoblasts' activities are more affected by increasing the nanoparticle content compared with fibroblasts. Animal studies indicate that both HA and mHA nanoparticles (10%) can reduce the expression of pro inflammatory cytokines after six weeks of implantation. In summary, this work highlights the potential of PDO/ LCL/HA nanocomposites as an excellent biomaterial for TL-bone interface tissue engineering applications.Dadun. Depósito Académico Digital Universidad de Navarra20232023-12-2020232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/68134reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/681342026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| title |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| spellingShingle |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb Silane modification Tendon enthesis Hydroxyapatite Nanocomposites Interface tissue |
| title_short |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| title_full |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| title_fullStr |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| title_full_unstemmed |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| title_sort |
Silane-modified hydroxyapatite nanoparticles incorporated into polydioxanone/poly(lactide-co-caprolactone) creates a novel toughened nanocomposite with improved material properties and in vivo inflammatory responses |
| dc.creator.none.fl_str_mv |
Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620 Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58e Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11ee Vahabli, E. (Ebrahim)|||/items/170429ea-8710-4c77-9011-d486b9d16341 Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41 Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342 Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5 Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3 |
| author |
Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb |
| author_facet |
Heidari, B.S. (Behzad Shiroud)|||/items/39f65451-bac4-45b6-84f5-a2c84440abbb Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620 Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58e Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11ee Vahabli, E. (Ebrahim)|||/items/170429ea-8710-4c77-9011-d486b9d16341 Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41 Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342 Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5 Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3 |
| author_role |
author |
| author2 |
Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620 Chen, P. (Peilin)|||/items/0f9a4c44-8645-45f8-9208-0a1d9c33c58e Ruan, R. (Rui)|||/items/97ec67de-1b93-410f-a313-6ed9efbf11ee Vahabli, E. (Ebrahim)|||/items/170429ea-8710-4c77-9011-d486b9d16341 Davachi, S.M. (Seyed Mohammad)|||/items/97b47088-68bd-4e95-bbbb-a0de40b4cf41 Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342 Zheng, M. (Minghao)|||/items/92ef8bb5-77fe-43ec-8cd8-600ae7928dc5 Doyle, B. (Barry)|||/items/cd05a898-8549-4ff5-a96d-f4a418fd6ea3 |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Silane modification Tendon enthesis Hydroxyapatite Nanocomposites Interface tissue |
| topic |
Silane modification Tendon enthesis Hydroxyapatite Nanocomposites Interface tissue |
| description |
The interface tissue between bone and soft tissues, such as tendon and ligament (TL), is highly prone to injury. Although different biomaterials have been developed for TL regeneration, few address the challenges of the TL bone interface. Here, we aim to develop novel hybrid nanocomposites based on poly(p-dioxanone) (PDO), poly (lactide-co-caprolactone) (LCL), and hydroxyapatite (HA) nanoparticles suitable for TL-bone interface repair. Nanocomposites, containing 3-10% of both unmodified and chemically modified hydroxyapatite (mHA) with a silane coupling agent. We then explored biocompatibility through in vitro and in vivo studies using a subcutaneous mouse model. Through different characterisation tests, we found that mHA increases tensile properties, creates rougher surfaces, and reduces crystallinity and hydrophilicity. Morphological observations indicate that mHA nanoparticles are attracted by PDO rather than LCL phase, resulting in a higher degradation rate for mHA group. We found that adding the 5% of nanoparticles gives a balance between the properties. In vitro experiments show that osteoblasts' activities are more affected by increasing the nanoparticle content compared with fibroblasts. Animal studies indicate that both HA and mHA nanoparticles (10%) can reduce the expression of pro inflammatory cytokines after six weeks of implantation. In summary, this work highlights the potential of PDO/ LCL/HA nanocomposites as an excellent biomaterial for TL-bone interface tissue engineering applications. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023-12-20 2023 2023-01-01 2023 2023-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/68134 |
| url |
https://hdl.handle.net/10171/68134 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869422320964075520 |
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15,301603 |