Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)

Fusarium head blight (FHB) is a complex disease of cereals caused by Fusarium species, which causes severe damages in terms of yield quality and quantity worldwide, and which produces mycotoxin contamination, posing a serious threat to public health. In the study presented herein, the antifungal act...

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Authors: Buzón Durán, Laura, Martín Gil, Jesús, Marcos Robles, José Luis, Fombellida Villafruela, Ángel, Pérez Lebeña, Eduardo, Martín Ramos, Pablo
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Universidad de Zaragoza
Repository:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:95444
Online Access:http://zaguan.unizar.es/record/95444
Access Level:Open access
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spelling Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)Buzón Durán, LauraMartín Gil, JesúsMarcos Robles, José LuisFombellida Villafruela, ÁngelPérez Lebeña, EduardoMartín Ramos, PabloFusarium head blight (FHB) is a complex disease of cereals caused by Fusarium species, which causes severe damages in terms of yield quality and quantity worldwide, and which produces mycotoxin contamination, posing a serious threat to public health. In the study presented herein, the antifungal activity against Fusarium culmorum of chitosan oligomers (COS)–amino acid conjugate complexes was investigated both in vitro and in vivo. The amino acids assayed were cysteine, glycine, proline and tyrosine. In vitro tests showed an enhancement of mycelial growth inhibition, with EC50 and EC90 effective concentration values ranging from 320 to 948 µg·mL−1 and from 1107 to 1407 µg·mL−1 respectively, for the conjugate complexes, as a result of the synergistic behavior between COS and the amino acids, tentatively ascribed to enhanced cell membrane damage originating from lipid peroxidation. Tests on colonies showed a maximum percentage reduction in the number of colonies at 1500 µg·mL−1 concentration, while grain tests were found to inhibit fungal growth, reducing deoxynivalenol content by 89%. The formulation that showed the best performance, i.e., the conjugate complex based on COS and tyrosine, was further investigated in a small-scale field trial with artificially inoculated spelt (Triticum spelta L.), and as a seed treatment to inhibit fungal growth in spelt seedlings. The field experiment showed that the chosen formulation induced a decrease in disease severity, with a control efficacy of 83.5%, while the seed tests showed that the treatment did not affect the percentage of germination and resulted in a lower incidence of root rot caused by the pathogen, albeit with a lower control efficacy (50%). Consequently, the reported conjugate complexes hold enough promise for crop protection applications to deserve further examination in larger field trials, with other Fusarium spp. pathogens and/or Triticum species.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/95444reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/UZ/UZ2019-TEC-07info:eu-repo/semantics/openAccessoai:zaguan.unizar.es:954442026-05-29T13:59:51Z
dc.title.none.fl_str_mv Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
title Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
spellingShingle Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
Buzón Durán, Laura
title_short Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
title_full Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
title_fullStr Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
title_full_unstemmed Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
title_sort Antifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
dc.creator.none.fl_str_mv Buzón Durán, Laura
Martín Gil, Jesús
Marcos Robles, José Luis
Fombellida Villafruela, Ángel
Pérez Lebeña, Eduardo
Martín Ramos, Pablo
author Buzón Durán, Laura
author_facet Buzón Durán, Laura
Martín Gil, Jesús
Marcos Robles, José Luis
Fombellida Villafruela, Ángel
Pérez Lebeña, Eduardo
Martín Ramos, Pablo
author_role author
author2 Martín Gil, Jesús
Marcos Robles, José Luis
Fombellida Villafruela, Ángel
Pérez Lebeña, Eduardo
Martín Ramos, Pablo
author2_role author
author
author
author
author
description Fusarium head blight (FHB) is a complex disease of cereals caused by Fusarium species, which causes severe damages in terms of yield quality and quantity worldwide, and which produces mycotoxin contamination, posing a serious threat to public health. In the study presented herein, the antifungal activity against Fusarium culmorum of chitosan oligomers (COS)–amino acid conjugate complexes was investigated both in vitro and in vivo. The amino acids assayed were cysteine, glycine, proline and tyrosine. In vitro tests showed an enhancement of mycelial growth inhibition, with EC50 and EC90 effective concentration values ranging from 320 to 948 µg·mL−1 and from 1107 to 1407 µg·mL−1 respectively, for the conjugate complexes, as a result of the synergistic behavior between COS and the amino acids, tentatively ascribed to enhanced cell membrane damage originating from lipid peroxidation. Tests on colonies showed a maximum percentage reduction in the number of colonies at 1500 µg·mL−1 concentration, while grain tests were found to inhibit fungal growth, reducing deoxynivalenol content by 89%. The formulation that showed the best performance, i.e., the conjugate complex based on COS and tyrosine, was further investigated in a small-scale field trial with artificially inoculated spelt (Triticum spelta L.), and as a seed treatment to inhibit fungal growth in spelt seedlings. The field experiment showed that the chosen formulation induced a decrease in disease severity, with a control efficacy of 83.5%, while the seed tests showed that the treatment did not affect the percentage of germination and resulted in a lower incidence of root rot caused by the pathogen, albeit with a lower control efficacy (50%). Consequently, the reported conjugate complexes hold enough promise for crop protection applications to deserve further examination in larger field trials, with other Fusarium spp. pathogens and/or Triticum species.
publishDate 2020
dc.date.none.fl_str_mv 2020
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dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/95444
url http://zaguan.unizar.es/record/95444
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/UZ/UZ2019-TEC-07
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
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dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
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