Viroid-like colonists of human microbiomes
[EN] Here, we describe "obelisks,"a class of heritable RNA elements sharing several properties: (1) apparently circular RNA 1 kb genome assemblies, (2) predicted rod-like genome-wide secondary structures, and (3) open reading frames encoding a novel "Oblin"protein...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/213268 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/213268 |
| Access Level: | acceso abierto |
| Palabra clave: | Human microbiomes RNA Genome Obelisks Protein |
| Sumario: | [EN] Here, we describe "obelisks,"a class of heritable RNA elements sharing several properties: (1) apparently circular RNA 1 kb genome assemblies, (2) predicted rod-like genome-wide secondary structures, and (3) open reading frames encoding a novel "Oblin"protein superfamily. A subset of obelisks includes a variant hammerhead self-cleaving ribozyme. Obelisks form their own phylogenetic group without detectable similarity to known biological agents. Surveying globally, we identified 29,959 distinct obelisks (clustered at 90% sequence identity) from diverse ecological niches. Obelisks are prevalent in human microbiomes, with detection in 7% (29/440) and 50% (17/32) of queried stool and oral metatranscriptomes, respectively. We establish Streptococcus sanguinis as a cellular host of a specific obelisk and find that this obelisk's maintenance is not essential for bacterial growth. Our observations identify obelisks as a class of diverse RNAs of yet-to-be- determined impact that have colonized and gone unnoticed in human and global microbiomes. |
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