Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces
Diversity inventories are critical to creating accurate species range maps and estimating population sizes, which in turn lead to better informed landscape and wildlife management decisions. Metabarcoding has facilitated large-scale environmental diversity surveys. However, the use of a metabarcodin...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2072/536470 |
| Acceso en línea: | http://hdl.handle.net/2072/536470 https://doi.org/10.1007/s10841-023-00479-7 |
| Access Level: | acceso abierto |
| Palabra clave: | Ocells Passeriformes Coleòpters Alimentació animal Metagenòmica Insectes saproxílics Excrements 59 |
| id |
ES_e1815b22682d80d9cd8756bf56e331bc |
|---|---|
| oai_identifier_str |
oai:recercat.cat:2072/536470 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird fecesBookwalter, JamieNiyas, Afaq M MohamedCaballero-López, BertaVillari, CaterinaClaramunt-López, BernatOcellsPasseriformesColeòptersAlimentació animalMetagenòmicaInsectes saproxílicsExcrements59Diversity inventories are critical to creating accurate species range maps and estimating population sizes, which in turn lead to better informed landscape and wildlife management decisions. Metabarcoding has facilitated large-scale environmental diversity surveys. However, the use of a metabarcoding approach with bird feces to survey arthropod diversity is still rela- tively undeveloped. The aim of this study was to see if and how a metabarcoding approach with bird feces could contribute to a saproxylic Coleoptera survey of traditional insect traps. We compared two methods of surveying saproxylic Coleoptera diversity (metabarcoding birds feces and deploying traditional traps) over two elevations in a mountain system. The two methods caught different species and different levels of functional guild richness. The metabarcoding method successfully recorded both distinct and overlapping portions of diversity from traditional collections, and the approach was also effec- tive in signaling the presence of both rare species and nine country records. Our results show that metabarcoding Passerine bird feces can be successful when used alongside traditional collection methods to capture a broad diversity of saproxylic Coleoptera. This method, however, has quantitative and qualitative limitations, including the inability to produce species abundance data as well as the generation of false positives and negatives due to biases within the metabarcoding pipeline. Implications for insect conservation As many terrestrial ecosystems lose insect diversity, insect diversity surveys are essential to understand the scope of the loss. Despite metabarcoding approach shortcomings, the declining costs and shorter survey and processing time required for this approach compared to traditional survey methods indicate that it can be a valuable addition to the toolkit for saproxylic Coleoptera diversity survey2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttp://hdl.handle.net/2072/536470https://doi.org/10.1007/s10841-023-00479-7RECERCAT (Dipòsit de la Recerca de Catalunya)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésJournal of insect conservation, 2023L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons:http://creativecommons.org/licenses/by/4.0/© The Author(s) 2023info:eu-repo/semantics/openAccessoai:recercat.cat:2072/5364702026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| title |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| spellingShingle |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces Bookwalter, Jamie Ocells Passeriformes Coleòpters Alimentació animal Metagenòmica Insectes saproxílics Excrements 59 |
| title_short |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| title_full |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| title_fullStr |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| title_full_unstemmed |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| title_sort |
Fecal matters: implementing classical Coleoptera species lists with metabarcoding data from passerine bird feces |
| dc.creator.none.fl_str_mv |
Bookwalter, Jamie Niyas, Afaq M Mohamed Caballero-López, Berta Villari, Caterina Claramunt-López, Bernat |
| author |
Bookwalter, Jamie |
| author_facet |
Bookwalter, Jamie Niyas, Afaq M Mohamed Caballero-López, Berta Villari, Caterina Claramunt-López, Bernat |
| author_role |
author |
| author2 |
Niyas, Afaq M Mohamed Caballero-López, Berta Villari, Caterina Claramunt-López, Bernat |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Ocells Passeriformes Coleòpters Alimentació animal Metagenòmica Insectes saproxílics Excrements 59 |
| topic |
Ocells Passeriformes Coleòpters Alimentació animal Metagenòmica Insectes saproxílics Excrements 59 |
| description |
Diversity inventories are critical to creating accurate species range maps and estimating population sizes, which in turn lead to better informed landscape and wildlife management decisions. Metabarcoding has facilitated large-scale environmental diversity surveys. However, the use of a metabarcoding approach with bird feces to survey arthropod diversity is still rela- tively undeveloped. The aim of this study was to see if and how a metabarcoding approach with bird feces could contribute to a saproxylic Coleoptera survey of traditional insect traps. We compared two methods of surveying saproxylic Coleoptera diversity (metabarcoding birds feces and deploying traditional traps) over two elevations in a mountain system. The two methods caught different species and different levels of functional guild richness. The metabarcoding method successfully recorded both distinct and overlapping portions of diversity from traditional collections, and the approach was also effec- tive in signaling the presence of both rare species and nine country records. Our results show that metabarcoding Passerine bird feces can be successful when used alongside traditional collection methods to capture a broad diversity of saproxylic Coleoptera. This method, however, has quantitative and qualitative limitations, including the inability to produce species abundance data as well as the generation of false positives and negatives due to biases within the metabarcoding pipeline. Implications for insect conservation As many terrestrial ecosystems lose insect diversity, insect diversity surveys are essential to understand the scope of the loss. Despite metabarcoding approach shortcomings, the declining costs and shorter survey and processing time required for this approach compared to traditional survey methods indicate that it can be a valuable addition to the toolkit for saproxylic Coleoptera diversity survey |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/2072/536470 https://doi.org/10.1007/s10841-023-00479-7 |
| url |
http://hdl.handle.net/2072/536470 https://doi.org/10.1007/s10841-023-00479-7 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Journal of insect conservation, 2023 |
| dc.rights.none.fl_str_mv |
© The Author(s) 2023 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
© The Author(s) 2023 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
13 p. application/pdf |
| dc.source.none.fl_str_mv |
RECERCAT (Dipòsit de la Recerca de Catalunya) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869422298747895808 |
| score |
15.811543 |