Ensayos de reproducción vegetativa de híbridos de castaño Castanea sativa x Castanea crenata
[EN] In order to get vegetative propagation of chestnut´s hybrids (Castanea sativa x Castanea crenata) fungus disease resistant experiments using both hard and soft cuttings were carried out. For hard cuttings the followings tests were set up: 1.- Cuttings treated for 24 hours under red light at 17-...
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 1952 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/61620 |
| Acceso en línea: | http://hdl.handle.net/10261/61620 |
| Access Level: | acceso abierto |
| Palabra clave: | Castanea sativa Castanea crenata Vegetative propagation Reproducción vegetativa Soft cuttings Hard cuttings Estacas duras Estacas foliosas |
| Sumario: | [EN] In order to get vegetative propagation of chestnut´s hybrids (Castanea sativa x Castanea crenata) fungus disease resistant experiments using both hard and soft cuttings were carried out. For hard cuttings the followings tests were set up: 1.- Cuttings treated for 24 hours under red light at 17-2OºC, with β-indolylacetic acid, α-naphthalencacetic acid, indolylpropionic acid, 2,4,5-trichlorophenoxyacetic acid and the sodium salt of α-naphthalencacetic acid at concentrations of 200, 125, 80, 40 and 20 ppm for each root-forming substance. 2.-Cuttings treated for 15 hours with a solution of copper sulfate, zinc sulfate, manganese sulfate, cobalt chloride and boric acid (each O,0Ol%0) with the addition 80, 40 or 2O ppm α-naphthalencacetic acid under same conditions as so (1). 3.- Cuttings treated at OºC. for 27 days followed by treatment with 5O ppm β-indolylacetic acid or a proprietary dust containing α-naphthalencacetic acid. For 1eafy cuttings, the followings tests were set up: 1.-Cuttings taken before mother-plant had flowered, treated with 100 and 50 ppm heteroauxin, α-naphthalencacetic acid or with a proprietary dust. Solution applied in dark a 19ºC. 2.-Cuttings, taken when mother-plant was at the early stages of fruit development, treated with 3.000 ppm α-naphthalencacetic acid for 1 minute and then rinsed in tap water or treated with two different proprietary dust. 3.-Cuttings taken from mother-plant with well developed fruit. Tests were made using a daily spraying ,with a Knop's modified solution, with 50 mgs. asparagine, 50 mgs. D l-α alanine, 2,5 g zinc sulfate and 8O g sucrose added to each liter. All cuttings were treated with a proprietary dust. With both the hard and soft cuttings, care was taken in the selection of most suitable material and in the handling. The time elapsing between cutting and application of treatments was never longer than 1-2 hours and the cuttings planted just after application of the root-forming substances. Hard cuttings were planted in well rinsed river sand in the open, inclined at angle and covered with cheesecloth. Leafy cuttings were planted vertically in a rooting medium covered with a crystal, placed in a room at 19-21°C; extra illumination was supplied with white fluorescent lamps. All cuttings were daily sprayed with water several times a day. No was employed in the rooting media hot bottom. Neither hard and soft cuttings gave positive results. Hard ones sprouted and grew shoots especially those treated with micronutrients; some shoots put out 3-8 well developed leaves during the period of 3-4 months; but no roots developed. Cold treatment appeared to be harmful, the cuttings developed buds only slightly. For soft cuttings, the 3rd test gave the best results, the cuttings living longer than in the other treatments, they callused well at the upper end but formed no roots. The only slight response to hormone treatment was found in cuttings with an apical bud, in which growth had ceased, when treated with a proprietary dust (α-naphthalencacetic acid) and sprayed daily with Knop´s modified solution, they developed but finally produced no roots. It would be desirable to set up further experiments using stronger concentrations of rooting forming substances, alone or in mixtures. In no case did the cuttings show any signs of injury through overdose. A higher degree of control of the external factors influencing root-formation would be very useful. |
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