Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine

The present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors to analyze the potential application of this novel ternary formulation to deliver the pCMS-EGFP plasmid into the rat retina. Binary vectors based on niosome/DNA and ternary vectors based on protamine/DNA/nios...

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Detalles Bibliográficos
Autores: Puras, G., Martínez-Navarrete, G., Mashal, M., Zárate, J., Aguirre, M., Ojeda, E., Grijalbo, S., Erijta, R., Díaz-Tahoces, Ariadna, Avilés-Trigueros, M., Fernández, E., Pedraz, J.L.
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/35414
Acceso en línea:https://hdl.handle.net/11000/35414
Access Level:acceso abierto
Palabra clave:gene therapy
nanotechnology
niosomes
nonviral vectors
protamine
retina
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spelling Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of ProtamineProtamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of ProtaminePuras, G.Martínez-Navarrete, G.Mashal, M.Zárate, J.Aguirre, M.Ojeda, E.Grijalbo, S.Erijta, R.Díaz-Tahoces, AriadnaAvilés-Trigueros, M.Fernández, E.Pedraz, J.L.gene therapynanotechnologyniosomesnonviral vectorsprotamineretinaThe present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors to analyze the potential application of this novel ternary formulation to deliver the pCMS-EGFP plasmid into the rat retina. Binary vectors based on niosome/DNA and ternary vectors based on protamine/DNA/niosomes were prepared and physicochemically characterized. In vitro experiments were performed in ARPE-19 cells. At 1:1:5 protamine/DNA/niosome mass ratio, the resulted ternary vectors had 150 nm size, positive charge, spherical morphology, and condensed, released, and protected the DNA against enzymatic digestion. The presence of protamine in the ternary vectors improved transfection efficiency, cell viability, and DNA condensation. After ocular administration, the EGFP expression was detected in different cell layers of the retina depending on the administration route without any sign of toxicity associated with the formulations. While subretinal administration transfected mainly photoreceptors and retinal pigment epithelial cells at the site of injection, intravitreal administration produced a more uniform distribution of the protein expression through the inner layers of the retina. The protein expression in the retina persisted for at least one month after both administrations. Our study highlights the flattering properties of protamine/DNA/niosome ternary vectors for efficient and safe gene delivery to the rat retina.ACS PublicationsDepartamentos de la UMH::Fisiología202520252015info:eu-repo/semantics/articleapplication/pdf14application/pdfhttps://hdl.handle.net/11000/35414reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheInglés10.1021/acs.molpharmaceut.5b00422info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/354142026-05-27T13:36:21Z
dc.title.none.fl_str_mv Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
title Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
spellingShingle Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
Puras, G.
gene therapy
nanotechnology
niosomes
nonviral vectors
protamine
retina
title_short Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
title_full Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
title_fullStr Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
title_full_unstemmed Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
title_sort Protamine/DNA/Niosome Ternary Nonviral Vectors for Gene Delivery to the Retina: The Role of Protamine
dc.creator.none.fl_str_mv Puras, G.
Martínez-Navarrete, G.
Mashal, M.
Zárate, J.
Aguirre, M.
Ojeda, E.
Grijalbo, S.
Erijta, R.
Díaz-Tahoces, Ariadna
Avilés-Trigueros, M.
Fernández, E.
Pedraz, J.L.
author Puras, G.
author_facet Puras, G.
Martínez-Navarrete, G.
Mashal, M.
Zárate, J.
Aguirre, M.
Ojeda, E.
Grijalbo, S.
Erijta, R.
Díaz-Tahoces, Ariadna
Avilés-Trigueros, M.
Fernández, E.
Pedraz, J.L.
author_role author
author2 Martínez-Navarrete, G.
Mashal, M.
Zárate, J.
Aguirre, M.
Ojeda, E.
Grijalbo, S.
Erijta, R.
Díaz-Tahoces, Ariadna
Avilés-Trigueros, M.
Fernández, E.
Pedraz, J.L.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Fisiología
dc.subject.none.fl_str_mv gene therapy
nanotechnology
niosomes
nonviral vectors
protamine
retina
topic gene therapy
nanotechnology
niosomes
nonviral vectors
protamine
retina
description The present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors to analyze the potential application of this novel ternary formulation to deliver the pCMS-EGFP plasmid into the rat retina. Binary vectors based on niosome/DNA and ternary vectors based on protamine/DNA/niosomes were prepared and physicochemically characterized. In vitro experiments were performed in ARPE-19 cells. At 1:1:5 protamine/DNA/niosome mass ratio, the resulted ternary vectors had 150 nm size, positive charge, spherical morphology, and condensed, released, and protected the DNA against enzymatic digestion. The presence of protamine in the ternary vectors improved transfection efficiency, cell viability, and DNA condensation. After ocular administration, the EGFP expression was detected in different cell layers of the retina depending on the administration route without any sign of toxicity associated with the formulations. While subretinal administration transfected mainly photoreceptors and retinal pigment epithelial cells at the site of injection, intravitreal administration produced a more uniform distribution of the protein expression through the inner layers of the retina. The protein expression in the retina persisted for at least one month after both administrations. Our study highlights the flattering properties of protamine/DNA/niosome ternary vectors for efficient and safe gene delivery to the rat retina.
publishDate 2015
dc.date.none.fl_str_mv 2015
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/35414
url https://hdl.handle.net/11000/35414
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 10.1021/acs.molpharmaceut.5b00422
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
14
application/pdf
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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