Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material
Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepare...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/97650 |
| Acceso en línea: | https://hdl.handle.net/11441/97650 https://doi.org/10.3390/pharmaceutics12050482 |
| Access Level: | acceso abierto |
| Palabra clave: | Gene therapy Metallo-liposomes Non-toxic nanocarriers Ruthenium(II)-based lipids Specificity by cancer cells Transfection process |
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Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic MaterialLebrón Romero, José AntonioOstos Marcos, Francisco JoséLópez López, ManuelMoyá Morán, María LuisaSales, CarlosGarcía, EncarnaciónGarcía Calderón, Clara BeatrizGarcía Calderón, MargaritaPeña Gómez, Mª JoséValle Rosado, IvánRomero Balestra, FernandoHuertas Sánchez, PabloLópez-Cornejo, María del PilarGene therapyMetallo-liposomesNon-toxic nanocarriersRuthenium(II)-based lipidsSpecificity by cancer cellsTransfection processGene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+ complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between Kapp and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide's condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.España Consejería de Educación y Ciencia de la Junta de Andalucía (Proyecto de Excelencia P12-FQM-1105, FQM-206 and FQM-274, and PI-0005-2018)España,, Universidad de Sevilla, VI Plan Propio Universidad de Sevilla (PP2018-10338)España Ministerio de Ciencia, Innovación y Universidades (RTI2018-100692-B-I00)MDPIQuímica Física2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/97650https://doi.org/10.3390/pharmaceutics12050482reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPharmaceutics, 12 (5), 482.(Proyecto de Excelencia P12-FQM-1105, FQM-206 and FQM-274, and PI-0005-2018)PP2018-10338RTI2018-100692-B-I00http://dx.doi.org/10.3390/pharmaceutics12050482info:eu-repo/semantics/openAccessoai:idus.us.es:11441/976502026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| title |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| spellingShingle |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material Lebrón Romero, José Antonio Gene therapy Metallo-liposomes Non-toxic nanocarriers Ruthenium(II)-based lipids Specificity by cancer cells Transfection process |
| title_short |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| title_full |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| title_fullStr |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| title_full_unstemmed |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| title_sort |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
| dc.creator.none.fl_str_mv |
Lebrón Romero, José Antonio Ostos Marcos, Francisco José López López, Manuel Moyá Morán, María Luisa Sales, Carlos García, Encarnación García Calderón, Clara Beatriz García Calderón, Margarita Peña Gómez, Mª José Valle Rosado, Iván Romero Balestra, Fernando Huertas Sánchez, Pablo López-Cornejo, María del Pilar |
| author |
Lebrón Romero, José Antonio |
| author_facet |
Lebrón Romero, José Antonio Ostos Marcos, Francisco José López López, Manuel Moyá Morán, María Luisa Sales, Carlos García, Encarnación García Calderón, Clara Beatriz García Calderón, Margarita Peña Gómez, Mª José Valle Rosado, Iván Romero Balestra, Fernando Huertas Sánchez, Pablo López-Cornejo, María del Pilar |
| author_role |
author |
| author2 |
Ostos Marcos, Francisco José López López, Manuel Moyá Morán, María Luisa Sales, Carlos García, Encarnación García Calderón, Clara Beatriz García Calderón, Margarita Peña Gómez, Mª José Valle Rosado, Iván Romero Balestra, Fernando Huertas Sánchez, Pablo López-Cornejo, María del Pilar |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Química Física |
| dc.subject.none.fl_str_mv |
Gene therapy Metallo-liposomes Non-toxic nanocarriers Ruthenium(II)-based lipids Specificity by cancer cells Transfection process |
| topic |
Gene therapy Metallo-liposomes Non-toxic nanocarriers Ruthenium(II)-based lipids Specificity by cancer cells Transfection process |
| description |
Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+ complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between Kapp and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide's condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/97650 https://doi.org/10.3390/pharmaceutics12050482 |
| url |
https://hdl.handle.net/11441/97650 https://doi.org/10.3390/pharmaceutics12050482 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Pharmaceutics, 12 (5), 482. (Proyecto de Excelencia P12-FQM-1105, FQM-206 and FQM-274, and PI-0005-2018) PP2018-10338 RTI2018-100692-B-I00 http://dx.doi.org/10.3390/pharmaceutics12050482 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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MDPI |
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MDPI |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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