Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces

Osseointegration of implants is crucial for the long-term success of oral implants. The periimplant bone formation by osteoblasts is strongly dependent on the local mechanical environment in the interface zone. Robust demands for energy are placed on osteoblasts during the adhesion process to solid...

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Detalles Bibliográficos
Autores: Salido Peracaula, Mercedes, Vilches Pérez, José Ignacio, Gonzalez, Juan L., Vilches Troya, José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/59853
Acceso en línea:http://hdl.handle.net/11441/59853
Access Level:acceso abierto
Palabra clave:Osteoblasts
Cell adhesion
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spelling Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfacesSalido Peracaula, MercedesVilches Pérez, José IgnacioGonzalez, Juan L.Vilches Troya, JoséOsteoblastsCell adhesionOsseointegration of implants is crucial for the long-term success of oral implants. The periimplant bone formation by osteoblasts is strongly dependent on the local mechanical environment in the interface zone. Robust demands for energy are placed on osteoblasts during the adhesion process to solid surfaces, and mitochondria are capital organelles in the production of most of the ATP needed for the process. We have assessed the relationship between osteoblast differentiation and mitochondrial bioenergetics in living cells grown on two different titanium surfaces, in order to provide valuable information for the design of material surfaces required for the development of the most appropriate osteogenic surface for osteoblastic anchorage. Combined backscattered and fluorescence confocal microscopy showed that in flat cells grown on a machined surface, highly energized mitochondria were distributed along the cell body. In contrast, cells grown on the rough surface emitted long protrusions in search of surface roughness, with actin stress fibers clearly polarized and highly energized mitochondria clustered at focal adhesion sites. This report using normal human osteoblastic cells indicates that these cells are especially sensitive to surface cues through energy production that enhances the necessary adhesion required for a successful osseointegration.Universidad de Murcia. Departamento de Biología Celular e HistologíaEstomatología2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/59853reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésHistology and Histopathology, 24 (10), 1275-1286.info:eu-repo/semantics/openAccessoai:idus.us.es:11441/598532026-06-17T12:51:07Z
dc.title.none.fl_str_mv Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
title Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
spellingShingle Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
Salido Peracaula, Mercedes
Osteoblasts
Cell adhesion
title_short Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
title_full Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
title_fullStr Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
title_full_unstemmed Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
title_sort Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
dc.creator.none.fl_str_mv Salido Peracaula, Mercedes
Vilches Pérez, José Ignacio
Gonzalez, Juan L.
Vilches Troya, José
author Salido Peracaula, Mercedes
author_facet Salido Peracaula, Mercedes
Vilches Pérez, José Ignacio
Gonzalez, Juan L.
Vilches Troya, José
author_role author
author2 Vilches Pérez, José Ignacio
Gonzalez, Juan L.
Vilches Troya, José
author2_role author
author
author
dc.contributor.none.fl_str_mv Estomatología
dc.subject.none.fl_str_mv Osteoblasts
Cell adhesion
topic Osteoblasts
Cell adhesion
description Osseointegration of implants is crucial for the long-term success of oral implants. The periimplant bone formation by osteoblasts is strongly dependent on the local mechanical environment in the interface zone. Robust demands for energy are placed on osteoblasts during the adhesion process to solid surfaces, and mitochondria are capital organelles in the production of most of the ATP needed for the process. We have assessed the relationship between osteoblast differentiation and mitochondrial bioenergetics in living cells grown on two different titanium surfaces, in order to provide valuable information for the design of material surfaces required for the development of the most appropriate osteogenic surface for osteoblastic anchorage. Combined backscattered and fluorescence confocal microscopy showed that in flat cells grown on a machined surface, highly energized mitochondria were distributed along the cell body. In contrast, cells grown on the rough surface emitted long protrusions in search of surface roughness, with actin stress fibers clearly polarized and highly energized mitochondria clustered at focal adhesion sites. This report using normal human osteoblastic cells indicates that these cells are especially sensitive to surface cues through energy production that enhances the necessary adhesion required for a successful osseointegration.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/59853
url http://hdl.handle.net/11441/59853
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Histology and Histopathology, 24 (10), 1275-1286.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad de Murcia. Departamento de Biología Celular e Histología
publisher.none.fl_str_mv Universidad de Murcia. Departamento de Biología Celular e Histología
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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