A moderate exposure to perfluorooctanoic acid causes persistent DNA damage and senescence in human epidermal HaCaT keratinocytes
Our results suggest that a single exposure to perfluorooctanoic acid causes concentration-dependent changes in cell proliferation that were not restored during a 48 h recovery period. Furthermore, we demonstrate that a moderate treatment with this perfluorochemical causes persistent DNA damage, whic...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/709920 |
| Acceso en línea: | http://hdl.handle.net/10486/709920 https://dx.doi.org/10.1016/j.fct.2018.09.020 |
| Access Level: | acceso abierto |
| Palabra clave: | Perfluorooctanoic acid HaCaT cells Cytotoxicity DNA damage Senescence Biología y Biomedicina / Biología |
| Sumario: | Our results suggest that a single exposure to perfluorooctanoic acid causes concentration-dependent changes in cell proliferation that were not restored during a 48 h recovery period. Furthermore, we demonstrate that a moderate treatment with this perfluorochemical causes persistent DNA damage, which ultimately leads to development of the senescence-associated secretory phenotype in HaCaT cells. This paper provides unprecedented data and insights regarding the cytotoxic effects of perfluorooctanoic acid in human cells that could be of special relevance for use in comparative in vitro-in vivo studies. Moreover, our findings highlight the importance of considering both the immediate and long-lasting or delayed cytotoxic responses caused by chemical exposure, to ensure the accurate identification of toxicity in cellbased systems |
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