Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates

A significant inoculum-size effect has been observed with piperacillin-tazobactam, and has been associated with β-lactamase production in extended-spectrum β-lactamase (ESBL) producers. This association has not been previously studied in the case of amoxycillin-clavulanate. Piperacillin-tazobactam a...

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Autores: López Cerero, Lorena, Picón, Eduardo, Morillo, E., Hernández, J. R., Docobo Pérez, Fernando, Pachón Díaz, Jerónimo, Rodríguez-Baño, Jesús, Pascual Hernández, Álvaro
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2010
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/171590
Acesso em linha:https://hdl.handle.net/11441/171590
https://doi.org/10.1111/j.1469-0691.2009.02893.x
Access Level:Acceso aberto
Palavra-chave:Amoxycillin–clavulanate
Extended-spectrum b-lactamase
Inoculum effect
Piperacillin–tazobactam
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spelling Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolatesLópez Cerero, LorenaPicón, EduardoMorillo, E.Hernández, J. R.Docobo Pérez, FernandoPachón Díaz, JerónimoRodríguez-Baño, JesúsPascual Hernández, ÁlvaroAmoxycillin–clavulanateExtended-spectrum b-lactamaseInoculum effectPiperacillin–tazobactamA significant inoculum-size effect has been observed with piperacillin-tazobactam, and has been associated with β-lactamase production in extended-spectrum β-lactamase (ESBL) producers. This association has not been previously studied in the case of amoxycillin-clavulanate. Piperacillin-tazobactam and amoxycillin-clavulanate were compared, using high inocula of susceptible strains either harbouring ESBLs or not. Two non-ESBL-producing and 15 amoxycillin-clavulanate-susceptible and piperacillin-tazobactam-susceptible ESBL-producing Escherichia coli isolates, and their respective transconjugants, were tested in dilution susceptibility tests using standard and 100-fold higher inocula. Three ESBL-producing strains and E. coli ATCC 25922 were selected for time-kill studies using standard and high initial inocula. At high inocula, MICs of piperacillin increased >eight-fold for non-ESBL-producing strains, and MICs of piperacillin-tazobactam (8 : 1 ratio or with tazobactam fixed at 4 mg/L) increased>eight-fold for all ESBL-producing strains. However, amoxycillin MICs were not affected by a high inoculum with non-ESBL-producing strains, whereas the MICs of amoxycillin-clavulanate (2 : 1 and 4 : 1) increased ≤four-fold for ESBL producers, using the broth and agar dilution methods. In kinetic studies at a high inoculum, amoxycillin and amoxycillin-clavulanate were bactericidal against E. coli ATCC 25922, whereas piperacillin and piperacillin-tazobactam yielded decreases of <1 log10 CFU/mL. Similarly, at a high inoculum, only amoxycillin-clavulanate was able to maintain bactericidal rates of killing over 24 h against the ESBL-positive E. coli isolates. The stability of amoxycillin-clavulanate and the contrasting results obtained with piperacillin-tazobactam against high inocula of ESBL-non-producing and ESBL-producing E. coli strains appear to be related to aspects other than the amount of β-lactamase production.ElsevierMicrobiologíaMedicinaCTS210: Resistencia a AntimicrobianosInstituto de Salud Carlos IIIJunta de AndalucíaMinisterio de Sanidad y Consumo. España2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/171590https://doi.org/10.1111/j.1469-0691.2009.02893.xreponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésClinical Microbiology and Infection, 16 (2), 132-136.75/04PI051019C03/14https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)61534-2/fulltextinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1715902026-06-17T12:51:07Z
dc.title.none.fl_str_mv Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
title Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
spellingShingle Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
López Cerero, Lorena
Amoxycillin–clavulanate
Extended-spectrum b-lactamase
Inoculum effect
Piperacillin–tazobactam
title_short Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
title_full Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
title_fullStr Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
title_full_unstemmed Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
title_sort Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates
dc.creator.none.fl_str_mv López Cerero, Lorena
Picón, Eduardo
Morillo, E.
Hernández, J. R.
Docobo Pérez, Fernando
Pachón Díaz, Jerónimo
Rodríguez-Baño, Jesús
Pascual Hernández, Álvaro
author López Cerero, Lorena
author_facet López Cerero, Lorena
Picón, Eduardo
Morillo, E.
Hernández, J. R.
Docobo Pérez, Fernando
Pachón Díaz, Jerónimo
Rodríguez-Baño, Jesús
Pascual Hernández, Álvaro
author_role author
author2 Picón, Eduardo
Morillo, E.
Hernández, J. R.
Docobo Pérez, Fernando
Pachón Díaz, Jerónimo
Rodríguez-Baño, Jesús
Pascual Hernández, Álvaro
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología
Medicina
CTS210: Resistencia a Antimicrobianos
Instituto de Salud Carlos III
Junta de Andalucía
Ministerio de Sanidad y Consumo. España
dc.subject.none.fl_str_mv Amoxycillin–clavulanate
Extended-spectrum b-lactamase
Inoculum effect
Piperacillin–tazobactam
topic Amoxycillin–clavulanate
Extended-spectrum b-lactamase
Inoculum effect
Piperacillin–tazobactam
description A significant inoculum-size effect has been observed with piperacillin-tazobactam, and has been associated with β-lactamase production in extended-spectrum β-lactamase (ESBL) producers. This association has not been previously studied in the case of amoxycillin-clavulanate. Piperacillin-tazobactam and amoxycillin-clavulanate were compared, using high inocula of susceptible strains either harbouring ESBLs or not. Two non-ESBL-producing and 15 amoxycillin-clavulanate-susceptible and piperacillin-tazobactam-susceptible ESBL-producing Escherichia coli isolates, and their respective transconjugants, were tested in dilution susceptibility tests using standard and 100-fold higher inocula. Three ESBL-producing strains and E. coli ATCC 25922 were selected for time-kill studies using standard and high initial inocula. At high inocula, MICs of piperacillin increased >eight-fold for non-ESBL-producing strains, and MICs of piperacillin-tazobactam (8 : 1 ratio or with tazobactam fixed at 4 mg/L) increased>eight-fold for all ESBL-producing strains. However, amoxycillin MICs were not affected by a high inoculum with non-ESBL-producing strains, whereas the MICs of amoxycillin-clavulanate (2 : 1 and 4 : 1) increased ≤four-fold for ESBL producers, using the broth and agar dilution methods. In kinetic studies at a high inoculum, amoxycillin and amoxycillin-clavulanate were bactericidal against E. coli ATCC 25922, whereas piperacillin and piperacillin-tazobactam yielded decreases of <1 log10 CFU/mL. Similarly, at a high inoculum, only amoxycillin-clavulanate was able to maintain bactericidal rates of killing over 24 h against the ESBL-positive E. coli isolates. The stability of amoxycillin-clavulanate and the contrasting results obtained with piperacillin-tazobactam against high inocula of ESBL-non-producing and ESBL-producing E. coli strains appear to be related to aspects other than the amount of β-lactamase production.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/171590
https://doi.org/10.1111/j.1469-0691.2009.02893.x
url https://hdl.handle.net/11441/171590
https://doi.org/10.1111/j.1469-0691.2009.02893.x
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Clinical Microbiology and Infection, 16 (2), 132-136.
75/04
PI051019
C03/14
https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)61534-2/fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
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