L-methionine supplementation modulates IgM+ B cell responses in rainbow trout
12 Pág.
| Autores: | , , , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/349581 |
| Acesso em linha: | http://hdl.handle.net/10261/349581 https://api.elsevier.com/content/abstract/scopus_id/85174941498 |
| Access Level: | acceso abierto |
| Palavra-chave: | IgM + B cells TNP-LPS Amino acids Lysine Methionine Rainbow trout Tryptophan |
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| dc.title.none.fl_str_mv |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| title |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| spellingShingle |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout Martín Lammerding, Diana IgM + B cells TNP-LPS Amino acids Lysine Methionine Rainbow trout Tryptophan |
| title_short |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| title_full |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| title_fullStr |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| title_full_unstemmed |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| title_sort |
L-methionine supplementation modulates IgM+ B cell responses in rainbow trout |
| dc.creator.none.fl_str_mv |
Martín Lammerding, Diana Ordás, M. Camino Carvalho, Inês Díaz-Rosales, Patricia Nuñez-Ortiz, Noelia Vicente-Gil, Samuel Arrogante, Aitor Zarza, Carles Machado, Marina Costas, Benjamin Tafalla, Carolina |
| author |
Martín Lammerding, Diana |
| author_facet |
Martín Lammerding, Diana Ordás, M. Camino Carvalho, Inês Díaz-Rosales, Patricia Nuñez-Ortiz, Noelia Vicente-Gil, Samuel Arrogante, Aitor Zarza, Carles Machado, Marina Costas, Benjamin Tafalla, Carolina |
| author_role |
author |
| author2 |
Ordás, M. Camino Carvalho, Inês Díaz-Rosales, Patricia Nuñez-Ortiz, Noelia Vicente-Gil, Samuel Arrogante, Aitor Zarza, Carles Machado, Marina Costas, Benjamin Tafalla, Carolina |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Agencia Estatal de Investigación (España) Comunidad de Madrid Fundação para a Ciência e a Tecnologia (Portugal) Martín Lammerding, Diana {0000-0002-9498-7904] Ordás, M. Camino [0000-0002-6544-6762] Díaz-Rosales, Patricia [0000-0001-6965-3702] Nuñez-Ortiz, Noelia [0000-0003-2524-0759] Costas, Benjamin [0000-0003-3758-0849] Tafalla, Carolina [0000-0002-0860-2976] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
IgM + B cells TNP-LPS Amino acids Lysine Methionine Rainbow trout Tryptophan |
| topic |
IgM + B cells TNP-LPS Amino acids Lysine Methionine Rainbow trout Tryptophan |
| description |
12 Pág. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2024 2024 |
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info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/349581 https://api.elsevier.com/content/abstract/scopus_id/85174941498 |
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http://hdl.handle.net/10261/349581 https://api.elsevier.com/content/abstract/scopus_id/85174941498 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113268RB-I00 2018-T2/BIO-10874/Talento program Centro de Investigación en Sanidad Animal (CISA) https://doi.org/10.3389/fimmu.2023.1264228 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Centro de Investigación en Sanidad Animal (CISA) |
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Frontiers Media |
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Frontiers Media |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869422239325093889 |
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L-methionine supplementation modulates IgM+ B cell responses in rainbow troutMartín Lammerding, DianaOrdás, M. CaminoCarvalho, InêsDíaz-Rosales, PatriciaNuñez-Ortiz, NoeliaVicente-Gil, SamuelArrogante, AitorZarza, CarlesMachado, MarinaCostas, BenjaminTafalla, CarolinaIgM + B cellsTNP-LPSAmino acidsLysineMethionineRainbow troutTryptophan12 Pág.The interest in dietary amino acids (AAs) as potential immunomodulators has been growing the recent years, since specific AAs are known to regulate key metabolic pathways of the immune response or increase the synthesis of some immune-related proteins. Methionine, tryptophan and lysine are among the ten essential AAs for fish, meaning that they cannot be produced endogenously and must be provided through the diet. To date, although dietary supplementation of fish with some of these AAs has been shown to have positive effects on some innate immune parameters and disease resistance, the effects that these AAs provoke on cells of the adaptive immune system remained unexplored. Hence, in the current study, we have investigated the effects of these three AAs on the functionality of rainbow trout (Oncorhynchus mykiss) IgM+ B cells. For this, splenic leukocytes were isolated from untreated adult rainbow trout and incubated in culture media additionally supplemented with different doses of methionine, tryptophan or lysine in the presence or absence of the model antigen TNP-LPS (2,4,6-trinitrophenyl hapten conjugated to lipopolysaccharide). The survival, IgM secreting capacity and proliferation of IgM+ B cells was then studied. In the case of methionine, the phagocytic capacity of IgM+ B cells was also determined. Our results demonstrate that methionine supplementation significantly increases the proliferative effects provoked by TNP-LPS and also up-regulates the number of cells secreting IgM, whereas tryptophan or lysine have either minor or even negative effects on rainbow trout IgM+ B cells. This increase in the number of IgM-secreting cells in response to methionine surplus was further verified in a feeding experiment, in which the beneficial effects of methionine on the specific response to anal immunization were also confirmed. The results presented demonstrate the beneficial effects of dietary supplementation with methionine on the adaptive immune responses of fish.The authors declare financial support was received for the research, authorship, and/or publication of this article. This work was part of the project PID2020-113268RB-I00 funded by MCNI/AEI/10.13039/501100011033. NN-O has a contract co-financed by INIA-CSIC and Comunidad de Madrid (Talento program grant 2018-T2/BIO-10874). This research was also supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal) within the scope of IMMUNAA (project PTDC/CVT-CVT/7741/2020), UIDB/04423/2020 and UIDP/04423/2020. IC and BC were supported by FCT, Portugal (2021.04867.BD and 2020.00290.CEECIND, respectively).Peer reviewedFrontiers MediaAgencia Estatal de Investigación (España)Comunidad de MadridFundação para a Ciência e a Tecnologia (Portugal)Martín Lammerding, Diana {0000-0002-9498-7904]Ordás, M. Camino [0000-0002-6544-6762]Díaz-Rosales, Patricia [0000-0001-6965-3702]Nuñez-Ortiz, Noelia [0000-0003-2524-0759]Costas, Benjamin [0000-0003-3758-0849]Tafalla, Carolina [0000-0002-0860-2976]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionCentro de Investigación en Sanidad Animal (CISA)http://hdl.handle.net/10261/349581https://api.elsevier.com/content/abstract/scopus_id/85174941498reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113268RB-I002018-T2/BIO-10874/Talento programCentro de Investigación en Sanidad Animal (CISA)https://doi.org/10.3389/fimmu.2023.1264228Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3495812026-05-22T06:33:51Z |
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15,811543 |