Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication

Producción Científica

Detalles Bibliográficos
Autores: Pagano, Aldo, Giannoni, Paolo, Zambotti, Adriana, Sánchez Romero, Diego, Ganfornina Álvarez, María Dolores, Gutiérrez, Gabriel, Randazzo, Nadia, Cancedda, Ranieri, Dozin, Beatrice
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/6081
Acceso en línea:https://doi.org/10.1016/j.gene.2004.02.001
http://uvadoc.uva.es/handle/10324/6081
Access Level:acceso abierto
Palabra clave:Lipocainas
Genética
Proteinas
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spelling Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplicationPagano, AldoGiannoni, PaoloZambotti, AdrianaSánchez Romero, DiegoGanfornina Álvarez, María DoloresGutiérrez, GabrielRandazzo, NadiaCancedda, RanieriDozin, BeatriceLipocainasGenéticaProteinasProducción CientíficaA novel lipocalin gene is here reported that represents the fourth member of a cluster we have identified in the chicken genome. This cluster also includes Chondrogenesis-Associated Lipocalins h and g (CALh, CALg) and Extracellular Fatty Acid Binding Protein (Ex- FABP). The new gene codes for a 22-kDa secreted protein with three cysteine residues and a series of sequence features well conserved in the lipocalin family. All the genes in the cluster are structurally similar presenting comparable exon/intron boundary positions and exon sizes. A phylogenetic analysis indicates the monophyletic grouping of these genes, and their relationship with the lipocalins a-1-microglobulin (A1mg), complement factor 8g chain (C8GC), prostaglandin D synthase (PGDS), and neutrophil-gelatinase-associated lipocalin (NGAL). The new cluster gene appears to be the ortholog of the mammalian C8GC and was thus named Ggal-C8GC. This orthology also suggests that this lipocalin was present in the ancestor common to reptiles and mammals. In addition to other expressing tissues, Ex-FABP, CALh and CALg genes are highly transcribed in chondrocytes at late stages of chondrogenesis during endochondral bone formation and/or upon inflammatory stimulation. Here, we show that they are also transcriptionally induced when chondrocytes are subjected to various biological events as cell quiescence, cell shape transition, and hormonal stimulation. By contrast, Ggal-C8GC transcripts are only barely detectable in chondrocytes, but are more abundant in liver, kidney, brain, heart, skeletal muscle and particularly in skin. Moreover, no expression induction was observed neither during chondrocyte differentiation, nor upon any of the stimulations mentioned above. This indicates that the Ggal-C8GC gene was co-opted for a novel function after the duplication events that gave rise to the cluster. The peculiar coordinated regulation of Ex-FABP, CALh and CALg, and the apparent divergent role of Ggal-C8GC suggest that these gene duplications may have been maintained during evolution by a sub-functionalization mechanism where some common function(s) are shared by several members of the cluster and some other specialized function(s) are unique to other members.Elsevier2004info:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1016/j.gene.2004.02.001http://uvadoc.uva.es/handle/10324/6081reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidInglésinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/60812026-06-13T12:44:47Z
dc.title.none.fl_str_mv Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
title Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
spellingShingle Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
Pagano, Aldo
Lipocainas
Genética
Proteinas
title_short Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
title_full Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
title_fullStr Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
title_full_unstemmed Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
title_sort Phylogeny and regulation of four lipocalin genes clustered in the chicken genome: evidence of a functional diversification after gene duplication
dc.creator.none.fl_str_mv Pagano, Aldo
Giannoni, Paolo
Zambotti, Adriana
Sánchez Romero, Diego
Ganfornina Álvarez, María Dolores
Gutiérrez, Gabriel
Randazzo, Nadia
Cancedda, Ranieri
Dozin, Beatrice
author Pagano, Aldo
author_facet Pagano, Aldo
Giannoni, Paolo
Zambotti, Adriana
Sánchez Romero, Diego
Ganfornina Álvarez, María Dolores
Gutiérrez, Gabriel
Randazzo, Nadia
Cancedda, Ranieri
Dozin, Beatrice
author_role author
author2 Giannoni, Paolo
Zambotti, Adriana
Sánchez Romero, Diego
Ganfornina Álvarez, María Dolores
Gutiérrez, Gabriel
Randazzo, Nadia
Cancedda, Ranieri
Dozin, Beatrice
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lipocainas
Genética
Proteinas
topic Lipocainas
Genética
Proteinas
description Producción Científica
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.gene.2004.02.001
http://uvadoc.uva.es/handle/10324/6081
url https://doi.org/10.1016/j.gene.2004.02.001
http://uvadoc.uva.es/handle/10324/6081
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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