Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development

35 p., figuras y bibliografía

Detalles Bibliográficos
Autores: Monroig, Óscar, Rotllant, Josep, Cerdá-Reverter, José Miguel, Dick, James R., Figueras Huerta, Antonio, Tocher, Douglas R.
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/43400
Acceso en línea:http://hdl.handle.net/10261/43400
Access Level:acceso abierto
Palabra clave:Development
Elongation
Elovl4
Fatty acid metabolism
Very long-chain fatty acids
Very long-chain polyunsaturated fatty acids
Zebrafish
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spelling Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic developmentMonroig, ÓscarRotllant, JosepCerdá-Reverter, José MiguelDick, James R.Figueras Huerta, AntonioTocher, Douglas R.DevelopmentElongationElovl4Fatty acid metabolismVery long-chain fatty acidsVery long-chain polyunsaturated fatty acidsZebrafish35 p., figuras y bibliografíaElovl4 is a fatty acyl elongase that participates in the biosynthesis of very long-chain fatty acids (≥C24), which are relatively abundant in skin (saturated chains), or retina, brain and testes (polyunsaturated chains) of mammals. In the present study we characterised two Elovl4 proteins, Elovl4a and Elovl4b, from zebrafish Danio rerio, and investigated their expression patterns during embryonic development. Heterologous expression in baker’s yeast showed that both zebrafish Elovl4 proteins efficiently elongated saturated fatty acids up to C36, with 26:0 appearing the preferred substrate as reported for human ELOVL4. Interestingly, activity for the elongation of PUFA substrates was only shown by Elovl4b, which effectively converted eicosapentaenoic (20:5n-3) and arachidonic (20:4n-6) acids to elongated polyenoic products up to C36. Furthermore, zebrafish Elovl4b may be involved in the biosynthesis of docosahexaenoic acid (22:6n-3, DHA) as it had the capacity to elongate 22:5n-3 to 24:5n-3 which can be subsequently desaturated and chain shortened to DHA in peroxisomes. The distinct functional roles of zebrafish Elovl4 proteins were also reflected in their spatial-temporal expression patterns during ontogeny. Analyses by whole-mount in situ hybridisation in zebrafish embryos showed that elovl4a was expressed in neuronal tissues (wide-spread distribution in the head area), with elovl4b specifically expressed in epiphysis (pineal gland) and photoreceptor cells in the retina. Similarly, tissue distribution in adults revealed that elovl4a transcripts were found in most tissues analysed, whereas elovl4b expression was essentially restricted to eye and gonads. Overall, the results suggest that zebrafish elovl4b resembles other mammalian orthologues in terms of function and expression patterns, whereas elovl4a may represent an alternative elongase not previously described in vertebrates.This research and OM were supported by a Marie Curie Intra European Fellowship within the 7th European Community Framework Programme (PIEF-GA-2008-220929, COBIAGENE). JR, JMCR and AF were funded by projects from the Spanish Ministry of Science and Innovation (AGL2008-00392/ACU, AGL2007-65744-C03-02 and Consolider-Ingenio-CSD2007-00002)Peer reviewedElsevier201120112010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/43400reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.bbalip.2010.06.005info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/434002026-05-22T06:33:51Z
dc.title.none.fl_str_mv Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
title Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
spellingShingle Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
Monroig, Óscar
Development
Elongation
Elovl4
Fatty acid metabolism
Very long-chain fatty acids
Very long-chain polyunsaturated fatty acids
Zebrafish
title_short Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
title_full Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
title_fullStr Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
title_full_unstemmed Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
title_sort Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development
dc.creator.none.fl_str_mv Monroig, Óscar
Rotllant, Josep
Cerdá-Reverter, José Miguel
Dick, James R.
Figueras Huerta, Antonio
Tocher, Douglas R.
author Monroig, Óscar
author_facet Monroig, Óscar
Rotllant, Josep
Cerdá-Reverter, José Miguel
Dick, James R.
Figueras Huerta, Antonio
Tocher, Douglas R.
author_role author
author2 Rotllant, Josep
Cerdá-Reverter, José Miguel
Dick, James R.
Figueras Huerta, Antonio
Tocher, Douglas R.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Development
Elongation
Elovl4
Fatty acid metabolism
Very long-chain fatty acids
Very long-chain polyunsaturated fatty acids
Zebrafish
topic Development
Elongation
Elovl4
Fatty acid metabolism
Very long-chain fatty acids
Very long-chain polyunsaturated fatty acids
Zebrafish
description 35 p., figuras y bibliografía
publishDate 2010
dc.date.none.fl_str_mv 2010
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/43400
url http://hdl.handle.net/10261/43400
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.bbalip.2010.06.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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