Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types

BACKGROUND: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan spec...

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Detalhes bibliográficos
Autores: Ylla Bou, Guillem, 1990-, Piulachs i Bagà, M. Dolors, Bellés, Xavier, 1952-
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/35403
Acesso em linha:http://hdl.handle.net/10230/35403
http://dx.doi.org/10.1186/s12864-017-4177-5
Access Level:acceso abierto
Palavra-chave:Blàstula
Blatèlids -- Genètica
Expressió gènica
Metamorfosi
MicroARN
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spelling Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband typesYlla Bou, Guillem, 1990-Piulachs i Bagà, M. DolorsBellés, Xavier, 1952-BlàstulaBlatèlids -- GenèticaExpressió gènicaMetamorfosiMicroARNBACKGROUND: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. RESULTS: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis, and the short germ-band holometabolan species Tribolium castaneum. The identification of B. germanica embryo small RNA sequences unveiled miRNAs not detected in previous studies, such as those of the MIR-309 family and 54 novel miRNAs. Four main waves of miRNA expression were recognized (with most miRNA changes occurring during the embryonic stages): the first from day 0 to day 1 of embryogenesis, the second during mid-embryogenesis (days 0-6), the third (with an acute expression peak) on day 2 of embryonic development, and the fourth during post-embryonic development. The second wave defined the boundaries of maternal-to-zygotic transition, with maternal mRNAs being cleared, presumably by Mir-309 and associated scavenger miRNAs. CONCLUSION: miRNAs follow well-defined patterns of expression over hemimetabolan ontogeny, patterns that are more diverse during embryonic development than during the nymphal stages. The results suggest that miRNAs play important roles in the developmental transitions between the embryonic stages of development (starting with maternal loading), during which they might influence the germ-band type and metamorphosis mode.This work was supported by the Spanish Ministry of Economy and Competitiveness (grants CGL2012–36251 and CGL2015–64727-P to X.B.), the Spanish Ministry of Science and Innovation (grants BFU2011–22404 and CGL2016–76011-R to M.D.P.) and the Catalan Government (2014 SGR 619). It also received financial assistance from the European Fund for Economic and Regional Development (FEDER funds to X.B. and M.D.P.)BioMed Central201820182017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/35403http://dx.doi.org/10.1186/s12864-017-4177-5reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBMC genomics. 2017 Oct 11;18(1):774info:eu-repo/grantAgreement/ES/3PN/CGL2012–36251info:eu-repo/grantAgreement/ES/1PE/CGL2015–64727-Pinfo:eu-repo/grantAgreement/ES/3PN/BFU2011–22404info:eu-repo/grantAgreement/ES/1PE/CGL2016–76011-R© Guillem Ylla, Maria-Dolors Piulachs and Xavier Belles 2017. Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/354032026-05-29T05:05:01Z
dc.title.none.fl_str_mv Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
title Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
spellingShingle Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
Ylla Bou, Guillem, 1990-
Blàstula
Blatèlids -- Genètica
Expressió gènica
Metamorfosi
MicroARN
title_short Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
title_full Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
title_fullStr Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
title_full_unstemmed Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
title_sort Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germband types
dc.creator.none.fl_str_mv Ylla Bou, Guillem, 1990-
Piulachs i Bagà, M. Dolors
Bellés, Xavier, 1952-
author Ylla Bou, Guillem, 1990-
author_facet Ylla Bou, Guillem, 1990-
Piulachs i Bagà, M. Dolors
Bellés, Xavier, 1952-
author_role author
author2 Piulachs i Bagà, M. Dolors
Bellés, Xavier, 1952-
author2_role author
author
dc.subject.none.fl_str_mv Blàstula
Blatèlids -- Genètica
Expressió gènica
Metamorfosi
MicroARN
topic Blàstula
Blatèlids -- Genètica
Expressió gènica
Metamorfosi
MicroARN
description BACKGROUND: Do miRNAs contribute to specify the germ-band type and the body structure in the insect embryo? Our goal was to address that issue by studying the changes in miRNA expression along the ontogeny of the German cockroach Blattella germanica, which is a short germ-band and hemimetabolan species. RESULTS: We sequenced small RNA libraries representing 11 developmental stages of B. germanica ontogeny (with especial emphasis on embryogenesis) and the changes in miRNA expression were examined. Data were compared with equivalent data for two long germ-band holometabolan species Drosophila melanogaster and Drosophila virilis, and the short germ-band holometabolan species Tribolium castaneum. The identification of B. germanica embryo small RNA sequences unveiled miRNAs not detected in previous studies, such as those of the MIR-309 family and 54 novel miRNAs. Four main waves of miRNA expression were recognized (with most miRNA changes occurring during the embryonic stages): the first from day 0 to day 1 of embryogenesis, the second during mid-embryogenesis (days 0-6), the third (with an acute expression peak) on day 2 of embryonic development, and the fourth during post-embryonic development. The second wave defined the boundaries of maternal-to-zygotic transition, with maternal mRNAs being cleared, presumably by Mir-309 and associated scavenger miRNAs. CONCLUSION: miRNAs follow well-defined patterns of expression over hemimetabolan ontogeny, patterns that are more diverse during embryonic development than during the nymphal stages. The results suggest that miRNAs play important roles in the developmental transitions between the embryonic stages of development (starting with maternal loading), during which they might influence the germ-band type and metamorphosis mode.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/35403
http://dx.doi.org/10.1186/s12864-017-4177-5
url http://hdl.handle.net/10230/35403
http://dx.doi.org/10.1186/s12864-017-4177-5
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BMC genomics. 2017 Oct 11;18(1):774
info:eu-repo/grantAgreement/ES/3PN/CGL2012–36251
info:eu-repo/grantAgreement/ES/1PE/CGL2015–64727-P
info:eu-repo/grantAgreement/ES/3PN/BFU2011–22404
info:eu-repo/grantAgreement/ES/1PE/CGL2016–76011-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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