Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes

Aldosterone (ALDO) may induce cardiac hypertrophy by nonhemodynamic mechanisms that are not completely defined. Cardiotrophin-1 (CT-1) is a cytokine that exerts hypertrophic actions on isolated cardiomyocytes and promotes cardiac hypertrophy in vivo. We investigated whether ALDO induces CT-1 express...

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Authors: Lopez-Andres, N. (Natalia)|||/items/80a86f6a-0bfc-4fa8-af4a-e6c1bd49e0be, Iñigo, C. (Carmen)|||/items/36348af3-3649-41b9-889e-83bf1768cd31, Gallego, I. (Idoia)|||/items/5151ad95-39a0-4319-85ee-c18e367c44a6, Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41, Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
Format: article
Publication Date:2008
Country:España
Institution:Universidad de Navarra
Repository:Dadun. Depósito Académico Digital de la Universidad de Navarra
Language:English
OAI Identifier:oai:dadun.unav.edu:10171/21853
Online Access:https://hdl.handle.net/10171/21853
Access Level:Open access
Keyword:Aldosterone/pharmacology
Cardiomegaly/physiopathology
Cytokines/genetics
Myocytes, Cardiac/physiology
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repository_id_str
spelling Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytesLopez-Andres, N. (Natalia)|||/items/80a86f6a-0bfc-4fa8-af4a-e6c1bd49e0beIñigo, C. (Carmen)|||/items/36348af3-3649-41b9-889e-83bf1768cd31Gallego, I. (Idoia)|||/items/5151ad95-39a0-4319-85ee-c18e367c44a6Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ecAldosterone/pharmacologyCardiomegaly/physiopathologyCytokines/geneticsCytokines/geneticsMyocytes, Cardiac/physiologyAldosterone (ALDO) may induce cardiac hypertrophy by nonhemodynamic mechanisms that are not completely defined. Cardiotrophin-1 (CT-1) is a cytokine that exerts hypertrophic actions on isolated cardiomyocytes and promotes cardiac hypertrophy in vivo. We investigated whether ALDO induces CT-1 expression in HL-1 cardiomyocytes aiming at the possibility that the cytokine is involved in ALDO-induced cardiomyocyte hypertrophy. mRNA and protein expression were quantified by RT-PCR and Western blot. Cardiomyocyte area, as an index of hypertrophy, was assayed by image analysis in phalloidin-stained HL-1 cells. ALDO addition to adult HL-1 cardiomyocytes increased (P<0.01) CT-1 mRNA and protein expression in a concentration-dependent manner. This effect was abrogated by actinomycin D, the mineralocorticoid and glucocorticoid receptor antagonists spironolactone and RU486, respectively, and the p38 MAPK blocker SB203580. CT-1 signaling pathway blockade with specific antibodies against the cytokine and its two receptor subunits avoided (P<0.01) alpha-sarcomeric actin and c-fos protein overexpression as well as cell size increase induced by ALDO in HL-1 cells. In vivo, a single ALDO injection acutely increased (P<0.01) the myocardial expression of CT-1 in C57BJ6 wild-type mice but not CT-1-null mice. The bolus of the mineralocorticoid increased (P<0.01) ANP and c-fos mRNA expression in the myocardium of wild-type mice, whereas no changes were observed in CT-1-null mice. In summary, ALDO induces CT-1 expression in adult HL-1 cardiomyocytes via genomic and nongenomic mechanisms. CT-1 up-regulation could have relevance in the direct hypertrophic effects of ALDO in cardiomyocytes.Endocrine SocietyDadun. Depósito Académico Digital Universidad de Navarra20122012-05-0220082008-01-0120082008-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/21853reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/218532026-06-21T12:47:57Z
dc.title.none.fl_str_mv Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
title Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
spellingShingle Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
Lopez-Andres, N. (Natalia)|||/items/80a86f6a-0bfc-4fa8-af4a-e6c1bd49e0be
Aldosterone/pharmacology
Cardiomegaly/physiopathology
Cytokines/genetics
Cytokines/genetics
Myocytes, Cardiac/physiology
title_short Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
title_full Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
title_fullStr Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
title_full_unstemmed Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
title_sort Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes
dc.creator.none.fl_str_mv Lopez-Andres, N. (Natalia)|||/items/80a86f6a-0bfc-4fa8-af4a-e6c1bd49e0be
Iñigo, C. (Carmen)|||/items/36348af3-3649-41b9-889e-83bf1768cd31
Gallego, I. (Idoia)|||/items/5151ad95-39a0-4319-85ee-c18e367c44a6
Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41
Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
author Lopez-Andres, N. (Natalia)|||/items/80a86f6a-0bfc-4fa8-af4a-e6c1bd49e0be
author_facet Lopez-Andres, N. (Natalia)|||/items/80a86f6a-0bfc-4fa8-af4a-e6c1bd49e0be
Iñigo, C. (Carmen)|||/items/36348af3-3649-41b9-889e-83bf1768cd31
Gallego, I. (Idoia)|||/items/5151ad95-39a0-4319-85ee-c18e367c44a6
Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41
Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
author_role author
author2 Iñigo, C. (Carmen)|||/items/36348af3-3649-41b9-889e-83bf1768cd31
Gallego, I. (Idoia)|||/items/5151ad95-39a0-4319-85ee-c18e367c44a6
Diez-Martinez, J. (Javier)|||/items/4f3a0e43-12bf-403d-9dc7-31fab0d11d41
Fortuño-Cebamanos, M.A. (María Antonia)|||/items/4dc78327-206a-4160-a352-90965670b0ec
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Aldosterone/pharmacology
Cardiomegaly/physiopathology
Cytokines/genetics
Cytokines/genetics
Myocytes, Cardiac/physiology
topic Aldosterone/pharmacology
Cardiomegaly/physiopathology
Cytokines/genetics
Cytokines/genetics
Myocytes, Cardiac/physiology
description Aldosterone (ALDO) may induce cardiac hypertrophy by nonhemodynamic mechanisms that are not completely defined. Cardiotrophin-1 (CT-1) is a cytokine that exerts hypertrophic actions on isolated cardiomyocytes and promotes cardiac hypertrophy in vivo. We investigated whether ALDO induces CT-1 expression in HL-1 cardiomyocytes aiming at the possibility that the cytokine is involved in ALDO-induced cardiomyocyte hypertrophy. mRNA and protein expression were quantified by RT-PCR and Western blot. Cardiomyocyte area, as an index of hypertrophy, was assayed by image analysis in phalloidin-stained HL-1 cells. ALDO addition to adult HL-1 cardiomyocytes increased (P<0.01) CT-1 mRNA and protein expression in a concentration-dependent manner. This effect was abrogated by actinomycin D, the mineralocorticoid and glucocorticoid receptor antagonists spironolactone and RU486, respectively, and the p38 MAPK blocker SB203580. CT-1 signaling pathway blockade with specific antibodies against the cytokine and its two receptor subunits avoided (P<0.01) alpha-sarcomeric actin and c-fos protein overexpression as well as cell size increase induced by ALDO in HL-1 cells. In vivo, a single ALDO injection acutely increased (P<0.01) the myocardial expression of CT-1 in C57BJ6 wild-type mice but not CT-1-null mice. The bolus of the mineralocorticoid increased (P<0.01) ANP and c-fos mRNA expression in the myocardium of wild-type mice, whereas no changes were observed in CT-1-null mice. In summary, ALDO induces CT-1 expression in adult HL-1 cardiomyocytes via genomic and nongenomic mechanisms. CT-1 up-regulation could have relevance in the direct hypertrophic effects of ALDO in cardiomyocytes.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01
2008
2008-01-01
2012
2012-05-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/21853
url https://hdl.handle.net/10171/21853
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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