Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles

Bacterial single cell protein (BSCP) obtained from methanotrophic bacteria was tested as an alternative protein source to fishmeal (FM) in compound diets for rainbow trout (Oncorhynchus mykiss) juveniles (initial body weight, BW = 11.3 ± 0.2 g, mean ± standard deviation). Different levels of FM repl...

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Detalhes bibliográficos
Autores: Ruiz, Alberto, Sanahuja, Ignasi, Thorringer, Nana W., Lynegaard, Julie, Ntokou, Eleni, Furones, Dolors, Gisbert, Enric
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/2537
Acesso em linha:http://hdl.handle.net/20.500.12327/2537
https://doi.org/10.1016/j.aquaculture.2023.739861
Access Level:acceso abierto
Palavra-chave:637
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oai_identifier_str oai:recercat.cat:20.500.12327/2537
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
title Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
spellingShingle Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
Ruiz, Alberto
637
title_short Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
title_full Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
title_fullStr Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
title_full_unstemmed Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
title_sort Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juveniles
dc.creator.none.fl_str_mv Ruiz, Alberto
Sanahuja, Ignasi
Thorringer, Nana W.
Lynegaard, Julie
Ntokou, Eleni
Furones, Dolors
Gisbert, Enric
author Ruiz, Alberto
author_facet Ruiz, Alberto
Sanahuja, Ignasi
Thorringer, Nana W.
Lynegaard, Julie
Ntokou, Eleni
Furones, Dolors
Gisbert, Enric
author_role author
author2 Sanahuja, Ignasi
Thorringer, Nana W.
Lynegaard, Julie
Ntokou, Eleni
Furones, Dolors
Gisbert, Enric
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
dc.subject.none.fl_str_mv 637
topic 637
description Bacterial single cell protein (BSCP) obtained from methanotrophic bacteria was tested as an alternative protein source to fishmeal (FM) in compound diets for rainbow trout (Oncorhynchus mykiss) juveniles (initial body weight, BW = 11.3 ± 0.2 g, mean ± standard deviation). Different levels of FM replacement (0, 25, 50, 75 and 100%) by a BSCP (0, 3.75, 7.5, 11.25 and 15% of Uniprotein® Aqua) were tested in isoproteic (crude protein: 42%) and isolipidic (crude fat: 21%) extruded diets. The trial was divided in two different parts, a nutritional trial that lasted 83 days during which the impact of experimental diets was evaluated in terms of key performance indicators (KPIs) associated to fish growth performance and feed utilization, which was followed by a challenge with a pathogenic bacterium (Aeromonas salmonicida subsp. salmonicida) to evaluate the immunological competence of fish fed the experimental diets (15 days). At the end of the nutritional trial, somatic growth in terms of body weight (BW) responded quadratically (P < 0.05) to the dietary inclusion of BCSP with the lowest BW values (183.4 ± 4.5 g) recorded in fish fed 100% FM replacement with BSCP, whereas the rest of dietary groups showed similar BW values (191.3–201.2 g). Feed intake was not affected by the level of FM replacement by BSCP, whereas feed conversion ratio (FCR) slightly increased with increasing levels of BSCP (P < 0.05), even though the lowest FCR values were found in fish fed 50% FM replacement by BSCP (0.79 ± 0.01) in comparison to the control group (0.81 ± 0.01). When using quadratic linear regression, the optimal dietary FM replacement by BSCP in terms of BW values was estimated as 41.7%. The nutritional quality of the fillet was not altered with experimental diets; all dietary groups showed similar amino acid levels as well as their content in HUFA n-3 and n-6 that remained unaltered (P > 0.05) and lipid nutritional indexes (P > 0.05). No negative effects were observed in the histological organization of the liver nor the proximate intestine, neither in the lipid peroxidation levels nor activity of antioxidative stress enzymes in the liver from fish fed increased inclusion levels of BSCP (P < 0.05). The replacement of FM by the tested BSCP obtained from methanotrophic bacteria did not cause an imbalance or dysbiosis in the intestinal microbiota in rainbow trout regardless of the level of FM replaced. The replacement of FM by BSCP at 50% improved disease resistance in rainbow trout exposed to furunculosis when compared to the control diet (cumulative mortality = 83.3 ± 7.2% vs. 49.2 ± 15.6%; P < 0.05), which suggested that this ingredient may also have some immunomodulatory properties that would protect fish from infections, although their mode of action deserves further attention. Current results indicated that the tested BSCP from methanotrophic bacteria is a safe, functional, and sustainable alternative protein source for aquafeeds.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/2537
https://doi.org/10.1016/j.aquaculture.2023.739861
url http://hdl.handle.net/20.500.12327/2537
https://doi.org/10.1016/j.aquaculture.2023.739861
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Aquaculture
EC/H2020/818312/EU/Unlocking new value from urban biowaste/VALUEWASTE
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 45
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Single cell protein from methanotrophic bacteria as an alternative healthy and functional protein source in aquafeeds, a holistic approach in rainbow trout (Oncorhynchus mykiss) juvenilesRuiz, AlbertoSanahuja, IgnasiThorringer, Nana W.Lynegaard, JulieNtokou, EleniFurones, DolorsGisbert, Enric637Bacterial single cell protein (BSCP) obtained from methanotrophic bacteria was tested as an alternative protein source to fishmeal (FM) in compound diets for rainbow trout (Oncorhynchus mykiss) juveniles (initial body weight, BW = 11.3 ± 0.2 g, mean ± standard deviation). Different levels of FM replacement (0, 25, 50, 75 and 100%) by a BSCP (0, 3.75, 7.5, 11.25 and 15% of Uniprotein® Aqua) were tested in isoproteic (crude protein: 42%) and isolipidic (crude fat: 21%) extruded diets. The trial was divided in two different parts, a nutritional trial that lasted 83 days during which the impact of experimental diets was evaluated in terms of key performance indicators (KPIs) associated to fish growth performance and feed utilization, which was followed by a challenge with a pathogenic bacterium (Aeromonas salmonicida subsp. salmonicida) to evaluate the immunological competence of fish fed the experimental diets (15 days). At the end of the nutritional trial, somatic growth in terms of body weight (BW) responded quadratically (P < 0.05) to the dietary inclusion of BCSP with the lowest BW values (183.4 ± 4.5 g) recorded in fish fed 100% FM replacement with BSCP, whereas the rest of dietary groups showed similar BW values (191.3–201.2 g). Feed intake was not affected by the level of FM replacement by BSCP, whereas feed conversion ratio (FCR) slightly increased with increasing levels of BSCP (P < 0.05), even though the lowest FCR values were found in fish fed 50% FM replacement by BSCP (0.79 ± 0.01) in comparison to the control group (0.81 ± 0.01). When using quadratic linear regression, the optimal dietary FM replacement by BSCP in terms of BW values was estimated as 41.7%. The nutritional quality of the fillet was not altered with experimental diets; all dietary groups showed similar amino acid levels as well as their content in HUFA n-3 and n-6 that remained unaltered (P > 0.05) and lipid nutritional indexes (P > 0.05). No negative effects were observed in the histological organization of the liver nor the proximate intestine, neither in the lipid peroxidation levels nor activity of antioxidative stress enzymes in the liver from fish fed increased inclusion levels of BSCP (P < 0.05). The replacement of FM by the tested BSCP obtained from methanotrophic bacteria did not cause an imbalance or dysbiosis in the intestinal microbiota in rainbow trout regardless of the level of FM replaced. The replacement of FM by BSCP at 50% improved disease resistance in rainbow trout exposed to furunculosis when compared to the control diet (cumulative mortality = 83.3 ± 7.2% vs. 49.2 ± 15.6%; P < 0.05), which suggested that this ingredient may also have some immunomodulatory properties that would protect fish from infections, although their mode of action deserves further attention. Current results indicated that the tested BSCP from methanotrophic bacteria is a safe, functional, and sustainable alternative protein source for aquafeeds.This work has been financed through the VALUEWASTE project that has received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. 818312. Alberto Ruiz was supported by a predoctoral grant (PRE2019-091259) and Ignasi Sanahuja was supported by a Juan de la Cierva postdoctoral contract (FJC2020-043933-I). Authors would like to express their gratitude to Magda Monllaó, Esteban Hernandez, Marta Sastre, Maria Curto, Olga Bellot and Edgar Bertomeu, staff from IRTA la Ràpita, for all their dedication and valuable assistance during the execution of the experiment.info:eu-repo/semantics/acceptedVersionElsevierProducció AnimalAqüicultura2023info:eu-repo/semantics/article45http://hdl.handle.net/20.500.12327/2537https://doi.org/10.1016/j.aquaculture.2023.739861reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAquacultureEC/H2020/818312/EU/Unlocking new value from urban biowaste/VALUEWASTEAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/25372026-05-29T05:05:01Z
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