Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease

Background and aims: Although there are several effective drugs for the treatment of Crohn's disease (CD), almost 70% of patients will require surgical resection during their lifetime. This procedure is not always curative, as endoscopic recurrence occurs in 65%-90% of patients in the first year...

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Autores: Oliver, Lia, Camps, Blau, Julià-Berkgvist, David, Amoedo Cibeira, Joan, Ramió Pujol, Sara, Malagón Rodríguez, Marta, Bahí, Anna, Torres, Paola, Domènech, Eugeni, Guardiola, Jordi, Serra Pagès, Mariona, Garcia-Gil, L. J., Aldeguer i Manté, Xavier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/23992
Acceso en línea:http://hdl.handle.net/10256/23992
Access Level:acceso abierto
Palabra clave:Crohn, Malaltia de
Crohn's disease
Intestins -- Inflamació
Inflammatory bowel diseases
Intestins -- Microbiologia
Intestines -- Microbiology
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spelling Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's diseaseOliver, LiaCamps, BlauJulià-Berkgvist, DavidAmoedo Cibeira, JoanRamió Pujol, SaraMalagón Rodríguez, MartaBahí, AnnaTorres, PaolaDomènech, EugeniGuardiola, JordiSerra Pagès, MarionaGarcia-Gil, L. J.Aldeguer i Manté, XavierCrohn, Malaltia deCrohn's diseaseIntestins -- InflamacióInflammatory bowel diseasesIntestins -- MicrobiologiaIntestines -- MicrobiologyBackground and aims: Although there are several effective drugs for the treatment of Crohn's disease (CD), almost 70% of patients will require surgical resection during their lifetime. This procedure is not always curative, as endoscopic recurrence occurs in 65%-90% of patients in the first year after surgery. The aetiology of the recurrence is unknown; however, several studies have shown how the resident microbiota is modified after surgery. The aim of this study was to evaluate samples from patients with Crohn's disease before and after an intestinal resection to determine whether there were differences in the abundance of different microbial markers, which may predict endoscopic recurrence at baseline. Methods: In this observational study, a stool sample was obtained from 25 patients with Crohn's disease before undergoing surgery, recruited at three Catalan hospitals. From each sample, DNA was purified and the relative abundance of nine microbial markers was quantified using qPCR. Results: An algorithm composed of four microbial markers (E. coli, F. prausnitzii phylogroup I, Bacteroidetes, and Eubacteria) showed a sensitivity and specificity of 90.91% and 85.71%, respectively, and a positive and negative predictive value of 83.33% and 92.31%, respectively. Conclusion: A microbial signature to determine patients who will have post-surgical recurrence was identified. This tool might be very useful in daily clinical practice, allowing the scheduling of personalized therapy and enabling preventive treatment only in patients who really require it2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/23992http://hdl.handle.net/10256/23992Frontiers in Molecular Medicine, 2023, vol. 3, art.núm.1046414Articles publicats (D-B)Oliver, Lia Camps, Blau Julià-Berkgvist, David Amoedo Cibeira, Joan Ramió Pujol, Sara Malagón Rodríguez, Marta Bahí, Anna Torres, Paola Domènech, Eugeni Guardiola, Jordi Serra Pagès, Mariona Garcia-Gil, L. J. Aldeguer i Manté, Xavier 2023 Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease Frontiers in Molecular Medicine 3 art.núm.1046414reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmmed.2023.1046414info:eu-repo/semantics/altIdentifier/eissn/2674-0095Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/239922026-05-29T05:05:01Z
dc.title.none.fl_str_mv Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
title Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
spellingShingle Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
Oliver, Lia
Crohn, Malaltia de
Crohn's disease
Intestins -- Inflamació
Inflammatory bowel diseases
Intestins -- Microbiologia
Intestines -- Microbiology
title_short Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
title_full Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
title_fullStr Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
title_full_unstemmed Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
title_sort Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease
dc.creator.none.fl_str_mv Oliver, Lia
Camps, Blau
Julià-Berkgvist, David
Amoedo Cibeira, Joan
Ramió Pujol, Sara
Malagón Rodríguez, Marta
Bahí, Anna
Torres, Paola
Domènech, Eugeni
Guardiola, Jordi
Serra Pagès, Mariona
Garcia-Gil, L. J.
Aldeguer i Manté, Xavier
author Oliver, Lia
author_facet Oliver, Lia
Camps, Blau
Julià-Berkgvist, David
Amoedo Cibeira, Joan
Ramió Pujol, Sara
Malagón Rodríguez, Marta
Bahí, Anna
Torres, Paola
Domènech, Eugeni
Guardiola, Jordi
Serra Pagès, Mariona
Garcia-Gil, L. J.
Aldeguer i Manté, Xavier
author_role author
author2 Camps, Blau
Julià-Berkgvist, David
Amoedo Cibeira, Joan
Ramió Pujol, Sara
Malagón Rodríguez, Marta
Bahí, Anna
Torres, Paola
Domènech, Eugeni
Guardiola, Jordi
Serra Pagès, Mariona
Garcia-Gil, L. J.
Aldeguer i Manté, Xavier
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Crohn, Malaltia de
Crohn's disease
Intestins -- Inflamació
Inflammatory bowel diseases
Intestins -- Microbiologia
Intestines -- Microbiology
topic Crohn, Malaltia de
Crohn's disease
Intestins -- Inflamació
Inflammatory bowel diseases
Intestins -- Microbiologia
Intestines -- Microbiology
description Background and aims: Although there are several effective drugs for the treatment of Crohn's disease (CD), almost 70% of patients will require surgical resection during their lifetime. This procedure is not always curative, as endoscopic recurrence occurs in 65%-90% of patients in the first year after surgery. The aetiology of the recurrence is unknown; however, several studies have shown how the resident microbiota is modified after surgery. The aim of this study was to evaluate samples from patients with Crohn's disease before and after an intestinal resection to determine whether there were differences in the abundance of different microbial markers, which may predict endoscopic recurrence at baseline. Methods: In this observational study, a stool sample was obtained from 25 patients with Crohn's disease before undergoing surgery, recruited at three Catalan hospitals. From each sample, DNA was purified and the relative abundance of nine microbial markers was quantified using qPCR. Results: An algorithm composed of four microbial markers (E. coli, F. prausnitzii phylogroup I, Bacteroidetes, and Eubacteria) showed a sensitivity and specificity of 90.91% and 85.71%, respectively, and a positive and negative predictive value of 83.33% and 92.31%, respectively. Conclusion: A microbial signature to determine patients who will have post-surgical recurrence was identified. This tool might be very useful in daily clinical practice, allowing the scheduling of personalized therapy and enabling preventive treatment only in patients who really require it
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/23992
http://hdl.handle.net/10256/23992
url http://hdl.handle.net/10256/23992
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmmed.2023.1046414
info:eu-repo/semantics/altIdentifier/eissn/2674-0095
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers in Molecular Medicine, 2023, vol. 3, art.núm.1046414
Articles publicats (D-B)
Oliver, Lia Camps, Blau Julià-Berkgvist, David Amoedo Cibeira, Joan Ramió Pujol, Sara Malagón Rodríguez, Marta Bahí, Anna Torres, Paola Domènech, Eugeni Guardiola, Jordi Serra Pagès, Mariona Garcia-Gil, L. J. Aldeguer i Manté, Xavier 2023 Definition of a microbial signature as a predictor of endoscopic post-surgical recurrence in patients with Crohn's disease Frontiers in Molecular Medicine 3 art.núm.1046414
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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