Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces

12 Pag., 3 Fig., 3 Tabl. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Detalles Bibliográficos
Autores: Silvar Casao, Cristina, Perovic, Dragan, Nussbaumer, Thomas, Spannagl, Manuel, Usadel, Björn, Casas Cendoya, Ana María, Igartua Arregui, Ernesto, Ordon, Frank
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/80818
Acceso en línea:http://hdl.handle.net/10261/80818
Access Level:acceso abierto
Palabra clave:Barley
Basic leucine zipper motif
Chromosomes
Chromosome 6h
Chromosome 7h
Controlled study
Gene identification
Genetic association
Genetic marker
Genome analysis
Nonhuman
Plant defense
Plant genome
Plant pathogen interaction
Powdery mildew
Protein function
Quantitative trait locus
Sequence homology
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spelling Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landracesSilvar Casao, CristinaPerovic, DraganNussbaumer, ThomasSpannagl, ManuelUsadel, BjörnCasas Cendoya, Ana MaríaIgartua Arregui, ErnestoOrdon, FrankBarleyBasic leucine zipper motifChromosomesChromosome 6hChromosome 7hControlled studyGene identificationGenetic associationGenetic markerGenome analysisNonhumanPlant defensePlant genomePlant pathogen interactionPowdery mildewProtein functionQuantitative trait locusSequence homologyddc:50012 Pag., 3 Fig., 3 Tabl. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Three quantitative trait loci (QTL) conferring broad spectrum resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei, were previously identified on chromosomes 7HS, 7HL and 6HL in the Spanish barley landrace-derived lines SBCC097 and SBCC145. In the present work, a genome-wide putative linear gene index of barley (Genome Zipper) and the first draft of the physical, genetic and functional sequence of the barley genome were used to go one step further in the shortening and explicit demarcation on the barley genome of these regions conferring resistance to powdery mildew as well as in the identification of candidate genes. First, a comparative analysis of the target regions to the barley Genome Zippers of chromosomes 7H and 6H allowed the development of 25 new gene-based molecular markers, which slightly better delimit the QTL intervals. These new markers provided the framework for anchoring of genetic and physical maps, figuring out the outline of the barley genome at the target regions in SBCC097 and SBCC145. The outermost flanking markers of QTLs on 7HS, 7HL and 6HL defined a physical area of 4 Mb, 3.7 Mb and 3.2 Mb, respectively. In total, 21, 10 and 16 genes on 7HS, 7HL and 6HL, respectively, could be interpreted as potential candidates to explain the resistance to powdery mildew, as they encode proteins of related functions with respect to the known pathogen defense-related processes. The majority of these were annotated as belonging to the NBS-LRR class or protein kinase family. © 2013 Silvar et al.Project ExpResBar, funded by the German Federal Ministry of Education and Research under grant number 0315702B within the KBBE-II call, and by the Spanish Ministry of Science and Innovation, grant number EUI2009-04075. The University of Coruña and the Deutscher Akademischer Austausch Dienst funded mobility fellowships for CS.Peer ReviewedPublic Library of Science20132013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/80818reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1371/journal.pone.0067336info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/808182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
title Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
spellingShingle Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
Silvar Casao, Cristina
Barley
Basic leucine zipper motif
Chromosomes
Chromosome 6h
Chromosome 7h
Controlled study
Gene identification
Genetic association
Genetic marker
Genome analysis
Nonhuman
Plant defense
Plant genome
Plant pathogen interaction
Powdery mildew
Protein function
Quantitative trait locus
Sequence homology
ddc:500
title_short Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
title_full Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
title_fullStr Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
title_full_unstemmed Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
title_sort Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
dc.creator.none.fl_str_mv Silvar Casao, Cristina
Perovic, Dragan
Nussbaumer, Thomas
Spannagl, Manuel
Usadel, Björn
Casas Cendoya, Ana María
Igartua Arregui, Ernesto
Ordon, Frank
author Silvar Casao, Cristina
author_facet Silvar Casao, Cristina
Perovic, Dragan
Nussbaumer, Thomas
Spannagl, Manuel
Usadel, Björn
Casas Cendoya, Ana María
Igartua Arregui, Ernesto
Ordon, Frank
author_role author
author2 Perovic, Dragan
Nussbaumer, Thomas
Spannagl, Manuel
Usadel, Björn
Casas Cendoya, Ana María
Igartua Arregui, Ernesto
Ordon, Frank
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Barley
Basic leucine zipper motif
Chromosomes
Chromosome 6h
Chromosome 7h
Controlled study
Gene identification
Genetic association
Genetic marker
Genome analysis
Nonhuman
Plant defense
Plant genome
Plant pathogen interaction
Powdery mildew
Protein function
Quantitative trait locus
Sequence homology
ddc:500
topic Barley
Basic leucine zipper motif
Chromosomes
Chromosome 6h
Chromosome 7h
Controlled study
Gene identification
Genetic association
Genetic marker
Genome analysis
Nonhuman
Plant defense
Plant genome
Plant pathogen interaction
Powdery mildew
Protein function
Quantitative trait locus
Sequence homology
ddc:500
description 12 Pag., 3 Fig., 3 Tabl. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/80818
url http://hdl.handle.net/10261/80818
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0067336
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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