A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle

Myogenin is the dominant transcriptional regulator of embryonic and fetal muscle differentiation and during maturation is profoundly down-regulated. We show that a highly conserved 17-bp DNA cis-acting sequence element located upstream of the myogenin promoter (myogHCE) is essential for postnatal re...

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Autores: Berghella, Libera, Angelis, Francesco De, Buysscher, Tristan de, Mortazavi, Ali, Biressi, Stefano, Forcales Fernàndez, Sonia-Vanina, Sirabella, Dario, Cossu, Claudia, Wold, Barbara J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/172284
Acceso en línea:https://hdl.handle.net/2445/172284
Access Level:acceso abierto
Palabra clave:Innervació
Músculs
Molècules
Innervation
Muscles
Molecules
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repository_id_str
spelling A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscleBerghella, LiberaAngelis, Francesco DeBuysscher, Tristan deMortazavi, AliBiressi, StefanoForcales Fernàndez, Sonia-VaninaSirabella, DarioCossu, ClaudiaWold, Barbara J.InnervacióMúsculsMolèculesInnervationMusclesMoleculesMyogenin is the dominant transcriptional regulator of embryonic and fetal muscle differentiation and during maturation is profoundly down-regulated. We show that a highly conserved 17-bp DNA cis-acting sequence element located upstream of the myogenin promoter (myogHCE) is essential for postnatal repression of myogenin in transgenic animals. We present multiple lines of evidence supporting the idea that repression is mediated by the Y-box protein MSY-3. Electroporation in vivo shows that myogHCE and MSY-3 are required for postnatal repression. We further show that, in the C2C12 cell culture system, ectopic MSY-3 can repress differentiation, while reduced MSY-3 promotes premature differentiation. MSY-3 binds myogHCE simultaneously with the homeodomain protein Pbx in postnatal innervated muscle. We therefore propose a model in which the myogHCE motif operates as a switch by specifying opposing functions; one that was shown previously is regulated by MyoD and Pbx and it specifies a chromatin opening, gene-activating function at the time myoblasts begin to differentiate; the other includes MYS-3 and Pbx, and it specifies a repression function that operates during and after postnatal muscle maturation in vivo and in myoblasts before they begin to differentiate.Cold Spring Harbor Laboratory Press2020202020082020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfhttps://hdl.handle.net/2445/172284Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1101/gad.468508Genes & Development, 2008, vol. 22, num. 15, p. 2125-2138https://doi.org/10.1101/gad.468508(c) Berghella, Libera et al., 2008info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1722842026-05-29T05:05:01Z
dc.title.none.fl_str_mv A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
title A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
spellingShingle A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
Berghella, Libera
Innervació
Músculs
Molècules
Innervation
Muscles
Molecules
title_short A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
title_full A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
title_fullStr A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
title_full_unstemmed A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
title_sort A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle
dc.creator.none.fl_str_mv Berghella, Libera
Angelis, Francesco De
Buysscher, Tristan de
Mortazavi, Ali
Biressi, Stefano
Forcales Fernàndez, Sonia-Vanina
Sirabella, Dario
Cossu, Claudia
Wold, Barbara J.
author Berghella, Libera
author_facet Berghella, Libera
Angelis, Francesco De
Buysscher, Tristan de
Mortazavi, Ali
Biressi, Stefano
Forcales Fernàndez, Sonia-Vanina
Sirabella, Dario
Cossu, Claudia
Wold, Barbara J.
author_role author
author2 Angelis, Francesco De
Buysscher, Tristan de
Mortazavi, Ali
Biressi, Stefano
Forcales Fernàndez, Sonia-Vanina
Sirabella, Dario
Cossu, Claudia
Wold, Barbara J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Innervació
Músculs
Molècules
Innervation
Muscles
Molecules
topic Innervació
Músculs
Molècules
Innervation
Muscles
Molecules
description Myogenin is the dominant transcriptional regulator of embryonic and fetal muscle differentiation and during maturation is profoundly down-regulated. We show that a highly conserved 17-bp DNA cis-acting sequence element located upstream of the myogenin promoter (myogHCE) is essential for postnatal repression of myogenin in transgenic animals. We present multiple lines of evidence supporting the idea that repression is mediated by the Y-box protein MSY-3. Electroporation in vivo shows that myogHCE and MSY-3 are required for postnatal repression. We further show that, in the C2C12 cell culture system, ectopic MSY-3 can repress differentiation, while reduced MSY-3 promotes premature differentiation. MSY-3 binds myogHCE simultaneously with the homeodomain protein Pbx in postnatal innervated muscle. We therefore propose a model in which the myogHCE motif operates as a switch by specifying opposing functions; one that was shown previously is regulated by MyoD and Pbx and it specifies a chromatin opening, gene-activating function at the time myoblasts begin to differentiate; the other includes MYS-3 and Pbx, and it specifies a repression function that operates during and after postnatal muscle maturation in vivo and in myoblasts before they begin to differentiate.
publishDate 2008
dc.date.none.fl_str_mv 2008
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/172284
url https://hdl.handle.net/2445/172284
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1101/gad.468508
Genes & Development, 2008, vol. 22, num. 15, p. 2125-2138
https://doi.org/10.1101/gad.468508
dc.rights.none.fl_str_mv (c) Berghella, Libera et al., 2008
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Berghella, Libera et al., 2008
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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