Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation

Cholestatic hepatitis C (CHC) is a severe form of hepatitis C virus (HCV) infection recurrence that leads to high graft loss rates early after liver transplantation (LT). To investigate the pathogenic mechanisms of CHC, we analysed HCV quasispecies in CHC patients compared to a control group (mild h...

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Autores: Gambato M, Gregori J, Quer J, Koutsoudakis G, González P, Caro-Pérez N, García-Cehic D, García-González N, González-Candelas F, Esteban JI, Crespo G, Navasa M, Forns X, Pérez-Del-Pulgar S
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p2619
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/2619
Access Level:acceso abierto
Palabra clave:hepatitis C virus
liver transplantation
cholestatic hepatitis C
quasispecies
deep sequencing
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network_name_str España
repository_id_str
spelling Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantationGambato MGregori JQuer JKoutsoudakis GGonzález PCaro-Pérez NGarcía-Cehic DGarcía-González NGonzález-Candelas FEsteban JICrespo GNavasa MForns XPérez-Del-Pulgar Shepatitis C virusliver transplantationcholestatic hepatitis Cquasispeciesdeep sequencingCholestatic hepatitis C (CHC) is a severe form of hepatitis C virus (HCV) infection recurrence that leads to high graft loss rates early after liver transplantation (LT). To investigate the pathogenic mechanisms of CHC, we analysed HCV quasispecies in CHC patients compared to a control group (mild hepatitis C recurrence) by deep pyrosequencing. At the time of LT, NS5B quasispecies complexity was similar between the two groups but, after LT, it decreased more sharply in CHC patients than in the control group. Interestingly, the major variant before LT propagated efficiently and remained as the dominant sequence after LT in 62 % of CHC patients versus 11 % of controls (P=0.031). Sequence analysis of the complete non-structural region in a limited number of patients revealed a potential 12 as signature specific to the CHC group. These data suggest that intrinsic molecular determinants in the circulating HCV quasispecies may provide a fitness advantage, contributing to the development of CHC.MICROBIOLOGY SOC2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/2619JOURNAL OF GENERAL VIROLOGYISSN: 00221317ISSNe: 14652099reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p26192026-06-11T12:45:17Z
dc.title.none.fl_str_mv Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
title Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
spellingShingle Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
Gambato M
hepatitis C virus
liver transplantation
cholestatic hepatitis C
quasispecies
deep sequencing
title_short Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
title_full Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
title_fullStr Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
title_full_unstemmed Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
title_sort Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation
dc.creator.none.fl_str_mv Gambato M
Gregori J
Quer J
Koutsoudakis G
González P
Caro-Pérez N
García-Cehic D
García-González N
González-Candelas F
Esteban JI
Crespo G
Navasa M
Forns X
Pérez-Del-Pulgar S
author Gambato M
author_facet Gambato M
Gregori J
Quer J
Koutsoudakis G
González P
Caro-Pérez N
García-Cehic D
García-González N
González-Candelas F
Esteban JI
Crespo G
Navasa M
Forns X
Pérez-Del-Pulgar S
author_role author
author2 Gregori J
Quer J
Koutsoudakis G
González P
Caro-Pérez N
García-Cehic D
García-González N
González-Candelas F
Esteban JI
Crespo G
Navasa M
Forns X
Pérez-Del-Pulgar S
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv hepatitis C virus
liver transplantation
cholestatic hepatitis C
quasispecies
deep sequencing
topic hepatitis C virus
liver transplantation
cholestatic hepatitis C
quasispecies
deep sequencing
description Cholestatic hepatitis C (CHC) is a severe form of hepatitis C virus (HCV) infection recurrence that leads to high graft loss rates early after liver transplantation (LT). To investigate the pathogenic mechanisms of CHC, we analysed HCV quasispecies in CHC patients compared to a control group (mild hepatitis C recurrence) by deep pyrosequencing. At the time of LT, NS5B quasispecies complexity was similar between the two groups but, after LT, it decreased more sharply in CHC patients than in the control group. Interestingly, the major variant before LT propagated efficiently and remained as the dominant sequence after LT in 62 % of CHC patients versus 11 % of controls (P=0.031). Sequence analysis of the complete non-structural region in a limited number of patients revealed a potential 12 as signature specific to the CHC group. These data suggest that intrinsic molecular determinants in the circulating HCV quasispecies may provide a fitness advantage, contributing to the development of CHC.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/2619
url https://fisabio.portalinvestigacion.com/publicaciones/2619
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MICROBIOLOGY SOC
publisher.none.fl_str_mv MICROBIOLOGY SOC
dc.source.none.fl_str_mv JOURNAL OF GENERAL VIROLOGY
ISSN: 00221317
ISSNe: 14652099
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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