Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles

In this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with glutaraldehyde, labelled CALA-MNP, (immobilization parameters: 84.1% ± 1.0 for immobilization yield and 208.0 ± 3.0 U/g ± 1.1 for deriv...

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Autores: Monteiro, Rodolpho R. C., Lima, Paula J. M., Pinheiro, Bruna B., Freire, Tiago M., Dutra, Lillian M. U., Fechine, Pierre B. A., Gonçalves, Luciana R. B., de Souza, Maria C. M., Santos, José C. S. dos, Fernández-Lafuente, Roberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/188976
Acceso en línea:http://hdl.handle.net/10261/188976
Access Level:acceso abierto
Palabra clave:Lipase A from Candida antarctica
Chitosan
Magnetic nanoparticles
Characterization
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spelling Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticlesMonteiro, Rodolpho R. C.Lima, Paula J. M.Pinheiro, Bruna B.Freire, Tiago M.Dutra, Lillian M. U.Fechine, Pierre B. A.Gonçalves, Luciana R. B.de Souza, Maria C. M.Santos, José C. S. dosFernández-Lafuente, RobertoLipase A from Candida antarcticaChitosanMagnetic nanoparticlesCharacterizationIn this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with glutaraldehyde, labelled CALA-MNP, (immobilization parameters: 84.1% ± 1.0 for immobilization yield and 208.0 ± 3.0 U/g ± 1.1 for derivative activity). CALA-MNP biocatalyst was characterized by X-ray Powder Diffraction (XRPD), Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetry (TG) and Scanning Electron Microscope (SEM), proving the incorporation of magnetite and the immobilization of CALA in the chitosan matrix. Besides, the immobilized biocatalyst showed a half-life 8–11 times higher than that of the soluble enzyme at pH 5–9. CALA showed the highest activity at pH 7, while CALA-MNP presented the highest activity at pH 10. The immobilized enzyme was more active than the free enzyme at all studied pH values, except pH 7.This research was funded by Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), grant numbers BP3-0139-00005.01.00/18 and PNE-0112-00048.01.00/16, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), grant numbers 422942/2016-2, 409058/2015-5 and 408790/2016-4, Coordenação de Aperfeiçoamento de Ensino Superior (CAPES-Finance Code 001) and MICIU, grant number CTQ2015-68951-C3-3-R.Peer reviewedMultidisciplinary Digital Publishing InstituteFundação Cearense de Apoio ao Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/188976reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-68951-C3-3-Rhttps://doi.org/10.3390/ijms20164018Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1889762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
title Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
spellingShingle Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
Monteiro, Rodolpho R. C.
Lipase A from Candida antarctica
Chitosan
Magnetic nanoparticles
Characterization
title_short Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
title_full Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
title_fullStr Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
title_full_unstemmed Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
title_sort Immobilization of lipase a from candida antarctica onto chitosan-coated magnetic nanoparticles
dc.creator.none.fl_str_mv Monteiro, Rodolpho R. C.
Lima, Paula J. M.
Pinheiro, Bruna B.
Freire, Tiago M.
Dutra, Lillian M. U.
Fechine, Pierre B. A.
Gonçalves, Luciana R. B.
de Souza, Maria C. M.
Santos, José C. S. dos
Fernández-Lafuente, Roberto
author Monteiro, Rodolpho R. C.
author_facet Monteiro, Rodolpho R. C.
Lima, Paula J. M.
Pinheiro, Bruna B.
Freire, Tiago M.
Dutra, Lillian M. U.
Fechine, Pierre B. A.
Gonçalves, Luciana R. B.
de Souza, Maria C. M.
Santos, José C. S. dos
Fernández-Lafuente, Roberto
author_role author
author2 Lima, Paula J. M.
Pinheiro, Bruna B.
Freire, Tiago M.
Dutra, Lillian M. U.
Fechine, Pierre B. A.
Gonçalves, Luciana R. B.
de Souza, Maria C. M.
Santos, José C. S. dos
Fernández-Lafuente, Roberto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lipase A from Candida antarctica
Chitosan
Magnetic nanoparticles
Characterization
topic Lipase A from Candida antarctica
Chitosan
Magnetic nanoparticles
Characterization
description In this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with glutaraldehyde, labelled CALA-MNP, (immobilization parameters: 84.1% ± 1.0 for immobilization yield and 208.0 ± 3.0 U/g ± 1.1 for derivative activity). CALA-MNP biocatalyst was characterized by X-ray Powder Diffraction (XRPD), Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetry (TG) and Scanning Electron Microscope (SEM), proving the incorporation of magnetite and the immobilization of CALA in the chitosan matrix. Besides, the immobilized biocatalyst showed a half-life 8–11 times higher than that of the soluble enzyme at pH 5–9. CALA showed the highest activity at pH 7, while CALA-MNP presented the highest activity at pH 10. The immobilized enzyme was more active than the free enzyme at all studied pH values, except pH 7.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/188976
url http://hdl.handle.net/10261/188976
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-68951-C3-3-R
https://doi.org/10.3390/ijms20164018
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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