Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic

The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of d- and l-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35°N to 5°S). Fluorescence in situ hybridization (FISH)...

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Detalles Bibliográficos
Autores: Varela, Marta María, Van-Aken, H.M., Herndl, G.J.
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/316623
Acceso en línea:http://hdl.handle.net/10261/316623
Access Level:acceso abierto
Palabra clave:Medio Marino
Centro Oceanográfico de A Coruña
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spelling Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical AtlanticVarela, Marta MaríaVan-Aken, H.M.Herndl, G.J.Medio MarinoCentro Oceanográfico de A CoruñaThe contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of d- and l-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35°N to 5°S). Fluorescence in situ hybridization (FISH) revealed that the overall abundance of SAR202 was ≤ 1 × 103 cells ml−1 in subsurface waters (100 m layer), increasing in the mesopelagic zone to 3 × 103 cells ml−1 and remaining fairly constant down to 4000 m depth. Overall, the percentage of total picoplankton identified as SAR202 increased from < 1% in subsurface waters to 10–20% in the bathypelagic waters. On average, members of the SAR202 cluster accounted for about 30% of the Bacteria in the bathypelagic waters, whereas in the mesopelagic and subsurface waters, SAR202 cells contributed < 5% to total bacterial abundance. The ratio of d-Asp : l-Asp uptake by the bulk picoplankton community increased from the subsurface layer (d-Asp : l-Asp uptake ratio ≈ 0.03) to the deeper layers reaching a ratio of ∼1 at 4000 m depth. Combining FISH with microautoradiography to determine the proportion of SAR202 cells taking up d-Asp versus l-Asp, we found that ≈ 30% of the SAR202 cells were taking up l-Asp throughout the water column while d-Asp was essentially not taken up by SAR202. This d-Asp : l-Asp uptake pattern of SAR202 cells is in contrast to that of the bulk bacterial and crenarchaeal community in the bathypelagic ocean, both sustaining a higher fraction of d-Asp-positive cells than l-Asp-positive cells. Thus, although the Chloroflexi-type SAR202 constitutes a major bathypelagic bacterial cluster, it does not contribute to the large fraction of d-Asp utilizing prokaryotic community in the meso- and bathypelagic waters of the North Atlantic, but rather utilizes preferentially l-amino acids.No202320232008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/316623reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de A Coruñahttps://ami-journals.onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2008.01627.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3166232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
title Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
spellingShingle Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
Varela, Marta María
Medio Marino
Centro Oceanográfico de A Coruña
title_short Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
title_full Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
title_fullStr Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
title_full_unstemmed Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
title_sort Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic
dc.creator.none.fl_str_mv Varela, Marta María
Van-Aken, H.M.
Herndl, G.J.
author Varela, Marta María
author_facet Varela, Marta María
Van-Aken, H.M.
Herndl, G.J.
author_role author
author2 Van-Aken, H.M.
Herndl, G.J.
author2_role author
author
dc.subject.none.fl_str_mv Medio Marino
Centro Oceanográfico de A Coruña
topic Medio Marino
Centro Oceanográfico de A Coruña
description The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of d- and l-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35°N to 5°S). Fluorescence in situ hybridization (FISH) revealed that the overall abundance of SAR202 was ≤ 1 × 103 cells ml−1 in subsurface waters (100 m layer), increasing in the mesopelagic zone to 3 × 103 cells ml−1 and remaining fairly constant down to 4000 m depth. Overall, the percentage of total picoplankton identified as SAR202 increased from < 1% in subsurface waters to 10–20% in the bathypelagic waters. On average, members of the SAR202 cluster accounted for about 30% of the Bacteria in the bathypelagic waters, whereas in the mesopelagic and subsurface waters, SAR202 cells contributed < 5% to total bacterial abundance. The ratio of d-Asp : l-Asp uptake by the bulk picoplankton community increased from the subsurface layer (d-Asp : l-Asp uptake ratio ≈ 0.03) to the deeper layers reaching a ratio of ∼1 at 4000 m depth. Combining FISH with microautoradiography to determine the proportion of SAR202 cells taking up d-Asp versus l-Asp, we found that ≈ 30% of the SAR202 cells were taking up l-Asp throughout the water column while d-Asp was essentially not taken up by SAR202. This d-Asp : l-Asp uptake pattern of SAR202 cells is in contrast to that of the bulk bacterial and crenarchaeal community in the bathypelagic ocean, both sustaining a higher fraction of d-Asp-positive cells than l-Asp-positive cells. Thus, although the Chloroflexi-type SAR202 constitutes a major bathypelagic bacterial cluster, it does not contribute to the large fraction of d-Asp utilizing prokaryotic community in the meso- and bathypelagic waters of the North Atlantic, but rather utilizes preferentially l-amino acids.
publishDate 2008
dc.date.none.fl_str_mv 2008
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/316623
url http://hdl.handle.net/10261/316623
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de A Coruña
https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2008.01627.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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