Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis
Purpose: To elucidate dysregulated proteins in keratoconus (KC) to provide a better understanding of the molecular mechanisms that lead to the development of the disease using sequential window acquisition of all theoretical mass spectra (SWATH-MS) as a protein quantification tool of the tear proteo...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Santiago de Compostela (USC) |
| Repositorio: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Idioma: | inglés |
| OAI Identifier: | oai:minerva.usc.gal:10347/45571 |
| Acceso en línea: | https://hdl.handle.net/10347/45571 |
| Access Level: | acceso abierto |
| Palabra clave: | Keratoconus Tear fluid Proteomics Mass spectrometry SWATH-MS |
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Tear proteomics in Keratoconus: a quantitative SWATH-MS analysisLópez López, MaiteRegueiro, Uxía Bravo López, Susana BelénChantada Vázquez, María del PilarVarela Fernández, RubénÁvila Gómez, PauloHervella Lorenzo, PabloLema Gesto, María IsabelKeratoconusTear fluidProteomicsMass spectrometrySWATH-MSPurpose: To elucidate dysregulated proteins in keratoconus (KC) to provide a better understanding of the molecular mechanisms that lead to the development of the disease using sequential window acquisition of all theoretical mass spectra (SWATH-MS) as a protein quantification tool of the tear proteomic profile. Methods: Prospective cross-sectional study that includes 25 keratoconic eyes and 25 healthy eyes. All participants underwent a clinical, tomographic, and aberrometric exam. Tear sample was collected using Schirmer strips and analyzed by liquid chromatography with tandem mass spectrometry. SWATH-MS was used as a quantification tool of the tear proteomic profile. The expression of the quantified proteins was compared between groups, and the biological and molecular functions of the dysregulated proteins as well as their functional relationships were studied by in silico analysis. Results: A total of 203 proteins were quantified in tear samples of patients with KC and control participants, of which 18 showed differential expression between groups (P < 0.05). An increase in the expression of 7 proteins and a decrease in the expression of 11 proteins were observed. Protein–protein interactions and gene ontology analysis showed the involvement of these dysregulated proteins in structural, inflammatory-immune, iron homeostasis, oxidative stress, and extracellular matrix proteolysis processes. Conclusions: Tear protein quantification has revealed the dysregulation of proteins involved in biological processes previously associated with KC. Among them, iron homeostasis should be highlighted as a relevant pathway in the KC pathophysiology, and it should be taken into account in the development of therapeutic targets to cope with tissue damage derived from iron accumulation and toxicityArvoUniversidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-CirúrxicasUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica20212021-08-2520212021-08-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/45571reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2© The Authors (2016-present). This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/455712026-06-15T12:47:27Z |
| dc.title.none.fl_str_mv |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| title |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| spellingShingle |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis López López, Maite Keratoconus Tear fluid Proteomics Mass spectrometry SWATH-MS |
| title_short |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| title_full |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| title_fullStr |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| title_full_unstemmed |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| title_sort |
Tear proteomics in Keratoconus: a quantitative SWATH-MS analysis |
| dc.creator.none.fl_str_mv |
López López, Maite Regueiro, Uxía Bravo López, Susana Belén Chantada Vázquez, María del Pilar Varela Fernández, Rubén Ávila Gómez, Paulo Hervella Lorenzo, Pablo Lema Gesto, María Isabel |
| author |
López López, Maite |
| author_facet |
López López, Maite Regueiro, Uxía Bravo López, Susana Belén Chantada Vázquez, María del Pilar Varela Fernández, Rubén Ávila Gómez, Paulo Hervella Lorenzo, Pablo Lema Gesto, María Isabel |
| author_role |
author |
| author2 |
Regueiro, Uxía Bravo López, Susana Belén Chantada Vázquez, María del Pilar Varela Fernández, Rubén Ávila Gómez, Paulo Hervella Lorenzo, Pablo Lema Gesto, María Isabel |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-Cirúrxicas Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
| dc.subject.none.fl_str_mv |
Keratoconus Tear fluid Proteomics Mass spectrometry SWATH-MS |
| topic |
Keratoconus Tear fluid Proteomics Mass spectrometry SWATH-MS |
| description |
Purpose: To elucidate dysregulated proteins in keratoconus (KC) to provide a better understanding of the molecular mechanisms that lead to the development of the disease using sequential window acquisition of all theoretical mass spectra (SWATH-MS) as a protein quantification tool of the tear proteomic profile. Methods: Prospective cross-sectional study that includes 25 keratoconic eyes and 25 healthy eyes. All participants underwent a clinical, tomographic, and aberrometric exam. Tear sample was collected using Schirmer strips and analyzed by liquid chromatography with tandem mass spectrometry. SWATH-MS was used as a quantification tool of the tear proteomic profile. The expression of the quantified proteins was compared between groups, and the biological and molecular functions of the dysregulated proteins as well as their functional relationships were studied by in silico analysis. Results: A total of 203 proteins were quantified in tear samples of patients with KC and control participants, of which 18 showed differential expression between groups (P < 0.05). An increase in the expression of 7 proteins and a decrease in the expression of 11 proteins were observed. Protein–protein interactions and gene ontology analysis showed the involvement of these dysregulated proteins in structural, inflammatory-immune, iron homeostasis, oxidative stress, and extracellular matrix proteolysis processes. Conclusions: Tear protein quantification has revealed the dysregulation of proteins involved in biological processes previously associated with KC. Among them, iron homeostasis should be highlighted as a relevant pathway in the KC pathophysiology, and it should be taken into account in the development of therapeutic targets to cope with tissue damage derived from iron accumulation and toxicity |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-08-25 2021 2021-08-25 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10347/45571 |
| url |
https://hdl.handle.net/10347/45571 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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Arvo |
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Arvo |
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reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname:Universidad de Santiago de Compostela (USC) |
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Universidad de Santiago de Compostela (USC) |
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Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
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Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
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