Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats
The benefits of intermittent hypobaric hypoxia (IHH) exposure for health and its potential use as a training tool are well-documented. However, since hypobaric hypoxia and cold are environmental factors always strongly associated in the biosphere, additive or synergistic adaptations could have evolv...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/244955 |
| Acceso en línea: | http://hdl.handle.net/10261/244955 |
| Access Level: | acceso abierto |
| Palabra clave: | Hypobaric Hypoxia Rats |
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Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in RatsSantocildes, GaroaViscor, GinésPagès, TeresaRamos-Romero, SaraTorres, Josep LluísTorrella, Joan RamonHypobaric HypoxiaRatsThe benefits of intermittent hypobaric hypoxia (IHH) exposure for health and its potential use as a training tool are well-documented. However, since hypobaric hypoxia and cold are environmental factors always strongly associated in the biosphere, additive or synergistic adaptations could have evolved in animals’ genomes. For that reason, the aim of the present study was to investigate body composition and hematological and muscle morphofunctional responses to simultaneous intermittent exposure to hypoxia and cold. Adult male rats were randomly divided into four groups: (1) control, maintained in normoxia at 25°C (CTRL); (2) IHH exposed 4 h/day at 4,500 m (HYPO); (3) intermittent cold exposed 4 h/day at 4°C (COLD); and (4) simultaneously cold and hypoxia exposed (COHY). At the end of 9 and 21 days of exposure, blood was withdrawn and gastrocnemius (GAS) and tibialis anterior muscles, perigonadal and brown adipose tissue, diaphragm, and heart were excised. GAS transversal sections were stained for myofibrillar ATPase and succinate dehydrogenase for fiber typing and for endothelial ATPase to assess capillarization. Hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), and glucose transporter 1 (GLUT1) from GAS samples were semi-quantified by Western blotting. COLD and HYPO underwent physiological adjustments such as higher brown adipose tissue weight and increase in blood-related oxygen transport parameters, while avoiding some negative effects of chronic exposure to cold and hypoxia, such as body weight and muscle mass loss. COHY presented an additive erythropoietic response and was prevented from right ventricle hypertrophy. Intermittent cold exposure induced muscle angiogenesis, and IHH seems to indicate better muscle oxygenation through fiber area reduction.This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness (DEP2013-48334-C2-1-P) and the University of Barcelona (graduate fellowship APIF 2016-2017 to GS).Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/244955reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/DEP2013-48334-C2-1-Phttps://doi.org/10.3389/fphys.2021.673095Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2449552026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| title |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| spellingShingle |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats Santocildes, Garoa Hypobaric Hypoxia Rats |
| title_short |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| title_full |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| title_fullStr |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| title_full_unstemmed |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| title_sort |
Physiological Effects of Intermittent Passive Exposure to Hypobaric Hypoxia and Cold in Rats |
| dc.creator.none.fl_str_mv |
Santocildes, Garoa Viscor, Ginés Pagès, Teresa Ramos-Romero, Sara Torres, Josep Lluís Torrella, Joan Ramon |
| author |
Santocildes, Garoa |
| author_facet |
Santocildes, Garoa Viscor, Ginés Pagès, Teresa Ramos-Romero, Sara Torres, Josep Lluís Torrella, Joan Ramon |
| author_role |
author |
| author2 |
Viscor, Ginés Pagès, Teresa Ramos-Romero, Sara Torres, Josep Lluís Torrella, Joan Ramon |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía y Competitividad (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Hypobaric Hypoxia Rats |
| topic |
Hypobaric Hypoxia Rats |
| description |
The benefits of intermittent hypobaric hypoxia (IHH) exposure for health and its potential use as a training tool are well-documented. However, since hypobaric hypoxia and cold are environmental factors always strongly associated in the biosphere, additive or synergistic adaptations could have evolved in animals’ genomes. For that reason, the aim of the present study was to investigate body composition and hematological and muscle morphofunctional responses to simultaneous intermittent exposure to hypoxia and cold. Adult male rats were randomly divided into four groups: (1) control, maintained in normoxia at 25°C (CTRL); (2) IHH exposed 4 h/day at 4,500 m (HYPO); (3) intermittent cold exposed 4 h/day at 4°C (COLD); and (4) simultaneously cold and hypoxia exposed (COHY). At the end of 9 and 21 days of exposure, blood was withdrawn and gastrocnemius (GAS) and tibialis anterior muscles, perigonadal and brown adipose tissue, diaphragm, and heart were excised. GAS transversal sections were stained for myofibrillar ATPase and succinate dehydrogenase for fiber typing and for endothelial ATPase to assess capillarization. Hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), and glucose transporter 1 (GLUT1) from GAS samples were semi-quantified by Western blotting. COLD and HYPO underwent physiological adjustments such as higher brown adipose tissue weight and increase in blood-related oxygen transport parameters, while avoiding some negative effects of chronic exposure to cold and hypoxia, such as body weight and muscle mass loss. COHY presented an additive erythropoietic response and was prevented from right ventricle hypertrophy. Intermittent cold exposure induced muscle angiogenesis, and IHH seems to indicate better muscle oxygenation through fiber area reduction. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/244955 |
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http://hdl.handle.net/10261/244955 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/DEP2013-48334-C2-1-P https://doi.org/10.3389/fphys.2021.673095 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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