Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120

Zur regulators control zinc homeostasis by repressing target genes under zinc-sufficient conditions in a wide variety of bacteria. This paper describes how part of a survey of duplicated genes led to the identification of the open reading frame all2473 as the gene encoding the Zur regulator of the c...

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Autores: Napolitano, Mauro, Rubio, Miguel A., Santamaría-Gómez, Javier, Olmedo-Verd, Elvira, Robinson, Nigel J., Luque, Ignacio
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/98093
Acceso en línea:http://hdl.handle.net/10261/98093
Access Level:acceso abierto
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spelling Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120Napolitano, MauroRubio, Miguel A.Santamaría-Gómez, JavierOlmedo-Verd, ElviraRobinson, Nigel J.Luque, IgnacioZur regulators control zinc homeostasis by repressing target genes under zinc-sufficient conditions in a wide variety of bacteria. This paper describes how part of a survey of duplicated genes led to the identification of the open reading frame all2473 as the gene encoding the Zur regulator of the cyanobacterium Anabaena sp. strain PCC 7120. All2473 binds to DNA in a zinc-dependent manner, and its DNA-binding sequence was characterized, which allowed us to determine the relative contribution of particular nucleotides to Zur binding. A zur mutant was found to be impaired in the regulation of zinc homeostasis, showing sensitivity to elevated concentrations of zinc but not other metals. In an effort to characterize the Zur regulon in Anabaena, 23 genes containing upstream putative Zur-binding sequences were identified and found to be regulated by Zur. These genes are organized in six single transcriptional units and six operons, some of them containing multiple Zur-regulated promoters. The identities of genes of the Zur regulon indicate that Anabaena adapts to conditions of zinc deficiency by replacing zinc metalloproteins with paralogues that fulfill the same function but presumably with a lower zinc demand, and with inducing putative metallochaperones and membrane transport systems likely being involved in the scavenging of extracellular zinc, including plasma membrane ABC transport systems and outer membrane TonB-dependent receptors. Among the Zur-regulated genes, the ones showing the highest induction level encode proteins of the outer membrane, suggesting a primary role for components of this cell compartment in the capture of zinc cations from the extracellular medium. © 2012, American Society for Microbiology.Peer ReviewedAmerican Society for MicrobiologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2014201420122014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/98093reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/980932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
title Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
spellingShingle Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
Napolitano, Mauro
title_short Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
title_full Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
title_fullStr Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
title_full_unstemmed Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
title_sort Characterization of the response to zinc deficiency in the cyanobacterium Anabaena sp. strain PCC 7120
dc.creator.none.fl_str_mv Napolitano, Mauro
Rubio, Miguel A.
Santamaría-Gómez, Javier
Olmedo-Verd, Elvira
Robinson, Nigel J.
Luque, Ignacio
author Napolitano, Mauro
author_facet Napolitano, Mauro
Rubio, Miguel A.
Santamaría-Gómez, Javier
Olmedo-Verd, Elvira
Robinson, Nigel J.
Luque, Ignacio
author_role author
author2 Rubio, Miguel A.
Santamaría-Gómez, Javier
Olmedo-Verd, Elvira
Robinson, Nigel J.
Luque, Ignacio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Zur regulators control zinc homeostasis by repressing target genes under zinc-sufficient conditions in a wide variety of bacteria. This paper describes how part of a survey of duplicated genes led to the identification of the open reading frame all2473 as the gene encoding the Zur regulator of the cyanobacterium Anabaena sp. strain PCC 7120. All2473 binds to DNA in a zinc-dependent manner, and its DNA-binding sequence was characterized, which allowed us to determine the relative contribution of particular nucleotides to Zur binding. A zur mutant was found to be impaired in the regulation of zinc homeostasis, showing sensitivity to elevated concentrations of zinc but not other metals. In an effort to characterize the Zur regulon in Anabaena, 23 genes containing upstream putative Zur-binding sequences were identified and found to be regulated by Zur. These genes are organized in six single transcriptional units and six operons, some of them containing multiple Zur-regulated promoters. The identities of genes of the Zur regulon indicate that Anabaena adapts to conditions of zinc deficiency by replacing zinc metalloproteins with paralogues that fulfill the same function but presumably with a lower zinc demand, and with inducing putative metallochaperones and membrane transport systems likely being involved in the scavenging of extracellular zinc, including plasma membrane ABC transport systems and outer membrane TonB-dependent receptors. Among the Zur-regulated genes, the ones showing the highest induction level encode proteins of the outer membrane, suggesting a primary role for components of this cell compartment in the capture of zinc cations from the extracellular medium. © 2012, American Society for Microbiology.
publishDate 2012
dc.date.none.fl_str_mv 2012
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/98093
url http://hdl.handle.net/10261/98093
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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