Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops

Double haploid technique aims to generate pure inbred lines for basic research and as commercial cultivars. This technique consists in a first obtention of haploid plants and a subsequent chromosome doubling, which can be separated in time or overlapped according to the procedure of each species. Si...

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Detalles Bibliográficos
Autores: Hooghvorst, Isidre, Nogués Mestres, Salvador
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/195534
Acceso en línea:https://hdl.handle.net/2445/195534
Access Level:acceso abierto
Palabra clave:Partenogènesi
Parthenogenesis
Conreus
Crops
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spelling Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major CropsHooghvorst, IsidreNogués Mestres, SalvadorPartenogènesiParthenogenesisConreusCropsDouble haploid technique aims to generate pure inbred lines for basic research and as commercial cultivars. This technique consists in a first obtention of haploid plants and a subsequent chromosome doubling, which can be separated in time or overlapped according to the procedure of each species. Since ever, much effort has been focused on haploid production via androgenesis, gynogenesis or parthenogenesis. Haploid plant obtention is the first step and requires more effort to optimize the haploid plant regeneration, and because of that chromosome doubling had usually been lagged behind. Nevertheless, chromosome doubling has recently been of renewed interest in double haploid research to increase double haploid plant obtention by optimizing and analysing different procedures. New antimitotic compounds, procedures and application methods are being published to ensure the success once haploid material has been regenerated. Moreover, haploid inducer-mediated genome-editing CRISPR/Cas9 system is a new breakthrough method to produce haploid plant material and requires chromosome doubling to succeed. This system could be of great importance for those species with non-successful haploid regeneration methods, or for recalcitrant species. In all cases, the new deployment of this system will demand a suitable chromosome doubling protocol. In this review we run through the existing double haploid and chromosome doubling methods to identify potential opportunities to enhance the breeding process in major crops.Springer Verlag2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/195534Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1007/s00299-020-02605-0Plant Cell Reports, 2021, vol. 40, num. 2, p. 255-270https://doi.org/10.1007/s00299-020-02605-0(c) Springer Verlag, 2021info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1955342026-05-27T06:46:51Z
dc.title.none.fl_str_mv Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
title Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
spellingShingle Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
Hooghvorst, Isidre
Partenogènesi
Parthenogenesis
Conreus
Crops
title_short Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
title_full Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
title_fullStr Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
title_full_unstemmed Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
title_sort Chromosome Doubling Methods in Double Haploid and Haploid Inducer-Mediated Genome-Editing System in Major Crops
dc.creator.none.fl_str_mv Hooghvorst, Isidre
Nogués Mestres, Salvador
author Hooghvorst, Isidre
author_facet Hooghvorst, Isidre
Nogués Mestres, Salvador
author_role author
author2 Nogués Mestres, Salvador
author2_role author
dc.subject.none.fl_str_mv Partenogènesi
Parthenogenesis
Conreus
Crops
topic Partenogènesi
Parthenogenesis
Conreus
Crops
description Double haploid technique aims to generate pure inbred lines for basic research and as commercial cultivars. This technique consists in a first obtention of haploid plants and a subsequent chromosome doubling, which can be separated in time or overlapped according to the procedure of each species. Since ever, much effort has been focused on haploid production via androgenesis, gynogenesis or parthenogenesis. Haploid plant obtention is the first step and requires more effort to optimize the haploid plant regeneration, and because of that chromosome doubling had usually been lagged behind. Nevertheless, chromosome doubling has recently been of renewed interest in double haploid research to increase double haploid plant obtention by optimizing and analysing different procedures. New antimitotic compounds, procedures and application methods are being published to ensure the success once haploid material has been regenerated. Moreover, haploid inducer-mediated genome-editing CRISPR/Cas9 system is a new breakthrough method to produce haploid plant material and requires chromosome doubling to succeed. This system could be of great importance for those species with non-successful haploid regeneration methods, or for recalcitrant species. In all cases, the new deployment of this system will demand a suitable chromosome doubling protocol. In this review we run through the existing double haploid and chromosome doubling methods to identify potential opportunities to enhance the breeding process in major crops.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/195534
url https://hdl.handle.net/2445/195534
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1007/s00299-020-02605-0
Plant Cell Reports, 2021, vol. 40, num. 2, p. 255-270
https://doi.org/10.1007/s00299-020-02605-0
dc.rights.none.fl_str_mv (c) Springer Verlag, 2021
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Springer Verlag, 2021
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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