Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)

Bactrocera oleae, the olive fruit fly, is a major pest of olive (Olea europaea L.) trees worldwide. Its presence can cause important losses, with consequences for the economies of countries that produce and export table olives and olive oil. Efforts to control olive fruit fly populations have, howev...

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Detalhes bibliográficos
Autores: Lantero, E., Matallanas, B., Ochando, M. D., Pascual, Susana, Callejas, C.
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/289666
Acesso em linha:http://hdl.handle.net/10261/289666
Access Level:acceso abierto
Palavra-chave:Olea europaea
Predation
Carabidae
Species-specific and sensitive primers
Feeding-trials
Cytochrome oxidase subunit I
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spelling Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)Lantero, E.Matallanas, B.Ochando, M. D.Pascual, SusanaCallejas, C.Olea europaeaPredationCarabidaeSpecies-specific and sensitive primersFeeding-trialsCytochrome oxidase subunit IBactrocera oleae, the olive fruit fly, is a major pest of olive (Olea europaea L.) trees worldwide. Its presence can cause important losses, with consequences for the economies of countries that produce and export table olives and olive oil. Efforts to control olive fruit fly populations have, however, been insufficient. Now more than ever, environmentally friendly alternatives need to be considered in potential control programs. Generalist predators could provide a way of managing this pest naturally. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of species-specific primers for detecting the presence of B. oleae DNA in the gut of predatory arthropods. All primers were tested for checking cross-reactive amplification of other fruit fly DNA and evaluated in heterospecific mixes of nucleic acids. All were found to be very sensitive for B. oleae. Subsequent feeding trials were conducted using one of the most abundant species of ground dwelling carabids in olive groves in south-eastern Madrid, Spain. These trials allowed determining that 253F-334R and 334F-253R primer pairs had the highest detection efficiency with an ID50 of around 78 h. These primers therefore provide a very useful tool for screening the gut contents of potential predators of B. oleae, and can thus reveal candidate species for the pest's biological control.Peer reviewedCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/289666reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDepartamento de Protección VegetalSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2896662026-05-22T06:33:51Z
dc.title.none.fl_str_mv Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
title Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
spellingShingle Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
Lantero, E.
Olea europaea
Predation
Carabidae
Species-specific and sensitive primers
Feeding-trials
Cytochrome oxidase subunit I
title_short Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
title_full Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
title_fullStr Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
title_full_unstemmed Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
title_sort Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera Tephritidae)
dc.creator.none.fl_str_mv Lantero, E.
Matallanas, B.
Ochando, M. D.
Pascual, Susana
Callejas, C.
author Lantero, E.
author_facet Lantero, E.
Matallanas, B.
Ochando, M. D.
Pascual, Susana
Callejas, C.
author_role author
author2 Matallanas, B.
Ochando, M. D.
Pascual, Susana
Callejas, C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Olea europaea
Predation
Carabidae
Species-specific and sensitive primers
Feeding-trials
Cytochrome oxidase subunit I
topic Olea europaea
Predation
Carabidae
Species-specific and sensitive primers
Feeding-trials
Cytochrome oxidase subunit I
description Bactrocera oleae, the olive fruit fly, is a major pest of olive (Olea europaea L.) trees worldwide. Its presence can cause important losses, with consequences for the economies of countries that produce and export table olives and olive oil. Efforts to control olive fruit fly populations have, however, been insufficient. Now more than ever, environmentally friendly alternatives need to be considered in potential control programs. Generalist predators could provide a way of managing this pest naturally. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of species-specific primers for detecting the presence of B. oleae DNA in the gut of predatory arthropods. All primers were tested for checking cross-reactive amplification of other fruit fly DNA and evaluated in heterospecific mixes of nucleic acids. All were found to be very sensitive for B. oleae. Subsequent feeding trials were conducted using one of the most abundant species of ground dwelling carabids in olive groves in south-eastern Madrid, Spain. These trials allowed determining that 253F-334R and 334F-253R primer pairs had the highest detection efficiency with an ID50 of around 78 h. These primers therefore provide a very useful tool for screening the gut contents of potential predators of B. oleae, and can thus reveal candidate species for the pest's biological control.
publishDate 2017
dc.date.none.fl_str_mv 2017
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/289666
url http://hdl.handle.net/10261/289666
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Departamento de Protección Vegetal
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
publisher.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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