Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction

We have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMPc-1, GMPc-2, MG 160) and two trans- (G...

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Autores: Alcalde, José, Bonay, Pedro, Roa, Ana, Vilaró, Senén, 1956-2005, Sandoval, Ignacio V.
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:1992
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/25208
Acesso em linha:https://hdl.handle.net/2445/25208
Access Level:Acceso aberto
Palavra-chave:Glicoproteïnes
Aparell de Golgi
Metabolisme cel·lular
Glycoproteins
Golgi apparatus
Cell metabolism
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spelling Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans directionAlcalde, JoséBonay, PedroRoa, AnaVilaró, Senén, 1956-2005Sandoval, Ignacio V.GlicoproteïnesAparell de GolgiMetabolisme cel·lularGlycoproteinsGolgi apparatusCell metabolismWe have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMPc-1, GMPc-2, MG 160) and two trans- (GMPt-1 and GMPt-2) Golgi membrane proteins during and after treatment of normal rat kidney (NRK) cells with brefeldin A (BFA). BFA induced complete disassembly of the cis/middle- and trans-Golgi complex and translocation of GMPc and GMPt to the ER. Cells treated for short times (3 min) with BFA showed extensive disorganization of both cis/middle- and trans-Golgi complexes. However, complete disorganization of the trans part required much longer incubations with the drug. Upon removal of BFA the Golgi complex was reassembled by a process consisting of three steps: (a) exist of cis/middle proteins from the ER and their accumulation into vesicular structures scattered throughout the cytoplasm; (b) gradual relocation and accumulation of the trans proteins in the vesicles containing the cis/middle proteins; and (c) assembly of the cisternae, and reconstruction of the Golgi complex within an area located in the vicinity of the centrosome from which the ER was excluded. Reconstruction of the cis/middle-Golgi complex occurred under temperature conditions inhibitory of the reorganization of the trans-Golgi complex, and was dependent on microtubules. Reconstruction of the trans-Golgi complex, disrupted with nocodazole after selective fusion of the cis/middle-Golgi complex with the ER, occurred after the release of cis/middle-Golgi proteins from the ER and the assembly of the cis/middle cisternae.Rockefeller University Press201220121992info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/25208Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció digital del document publicat a: http://dx.doi.org/10.1083/jcb.116.1.69Journal of Cell Biology, 1992, vol. 116, núm. 1, p. 69-83http://dx.doi.org/10.1083/jcb.116.1.69(c) Rockefeller University Press, 1992info:eu-repo/semantics/openAccessoai:recercat.cat:2445/252082026-05-29T05:05:01Z
dc.title.none.fl_str_mv Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
title Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
spellingShingle Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
Alcalde, José
Glicoproteïnes
Aparell de Golgi
Metabolisme cel·lular
Glycoproteins
Golgi apparatus
Cell metabolism
title_short Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
title_full Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
title_fullStr Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
title_full_unstemmed Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
title_sort Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction
dc.creator.none.fl_str_mv Alcalde, José
Bonay, Pedro
Roa, Ana
Vilaró, Senén, 1956-2005
Sandoval, Ignacio V.
author Alcalde, José
author_facet Alcalde, José
Bonay, Pedro
Roa, Ana
Vilaró, Senén, 1956-2005
Sandoval, Ignacio V.
author_role author
author2 Bonay, Pedro
Roa, Ana
Vilaró, Senén, 1956-2005
Sandoval, Ignacio V.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Glicoproteïnes
Aparell de Golgi
Metabolisme cel·lular
Glycoproteins
Golgi apparatus
Cell metabolism
topic Glicoproteïnes
Aparell de Golgi
Metabolisme cel·lular
Glycoproteins
Golgi apparatus
Cell metabolism
description We have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMPc-1, GMPc-2, MG 160) and two trans- (GMPt-1 and GMPt-2) Golgi membrane proteins during and after treatment of normal rat kidney (NRK) cells with brefeldin A (BFA). BFA induced complete disassembly of the cis/middle- and trans-Golgi complex and translocation of GMPc and GMPt to the ER. Cells treated for short times (3 min) with BFA showed extensive disorganization of both cis/middle- and trans-Golgi complexes. However, complete disorganization of the trans part required much longer incubations with the drug. Upon removal of BFA the Golgi complex was reassembled by a process consisting of three steps: (a) exist of cis/middle proteins from the ER and their accumulation into vesicular structures scattered throughout the cytoplasm; (b) gradual relocation and accumulation of the trans proteins in the vesicles containing the cis/middle proteins; and (c) assembly of the cisternae, and reconstruction of the Golgi complex within an area located in the vicinity of the centrosome from which the ER was excluded. Reconstruction of the cis/middle-Golgi complex occurred under temperature conditions inhibitory of the reorganization of the trans-Golgi complex, and was dependent on microtubules. Reconstruction of the trans-Golgi complex, disrupted with nocodazole after selective fusion of the cis/middle-Golgi complex with the ER, occurred after the release of cis/middle-Golgi proteins from the ER and the assembly of the cis/middle cisternae.
publishDate 1992
dc.date.none.fl_str_mv 1992
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/25208
url https://hdl.handle.net/2445/25208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció digital del document publicat a: http://dx.doi.org/10.1083/jcb.116.1.69
Journal of Cell Biology, 1992, vol. 116, núm. 1, p. 69-83
http://dx.doi.org/10.1083/jcb.116.1.69
dc.rights.none.fl_str_mv (c) Rockefeller University Press, 1992
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Rockefeller University Press, 1992
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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