Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

Enzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use the technology to improve enzyme performance, still today we are far from full exploitation of the field. One clear reason is that many evaluate immobilization based on only a few experi...

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Detalles Bibliográficos
Autores: Woodley, J.M., Bolívar Bolívar, Juan Manuel, Fernandez-Lafuente, R.
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/102214
Acceso en línea:https://hdl.handle.net/20.500.14352/102214
Access Level:acceso abierto
Palabra clave:66.0
620
Ingeniería química
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3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
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spelling Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilizationWoodley, J.M.Bolívar Bolívar, Juan ManuelFernandez-Lafuente, R.66.0620Ingeniería químicaQuímica23 Química3302 Tecnología Bioquímica3303 Ingeniería y Tecnología QuímicasEnzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use the technology to improve enzyme performance, still today we are far from full exploitation of the field. One clear reason is that many evaluate immobilization based on only a few experiments that are not always well-designed. In contrast to many other reviews on the subject, here we highlight the pitfalls of using incorrectly designed immobilization protocols and explain why in many cases sub-optimal results are obtained. We also describe solutions to overcome these challenges and come to the conclusion that recent developments in material science, bioprocess engineering and protein science continue to open new opportunities for the future. In this way, enzyme immobilization, far from being a mature discipline, remains as a subject of high interest and where intense research is still necessary to take full advantage of the possibilities.Universidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102214reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1022142026-06-02T12:44:21Z
dc.title.none.fl_str_mv Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
title Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
spellingShingle Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
Woodley, J.M.
66.0
620
Ingeniería química
Química
23 Química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
title_short Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
title_full Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
title_fullStr Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
title_full_unstemmed Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
title_sort Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
dc.creator.none.fl_str_mv Woodley, J.M.
Bolívar Bolívar, Juan Manuel
Fernandez-Lafuente, R.
author Woodley, J.M.
author_facet Woodley, J.M.
Bolívar Bolívar, Juan Manuel
Fernandez-Lafuente, R.
author_role author
author2 Bolívar Bolívar, Juan Manuel
Fernandez-Lafuente, R.
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 66.0
620
Ingeniería química
Química
23 Química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
topic 66.0
620
Ingeniería química
Química
23 Química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
description Enzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use the technology to improve enzyme performance, still today we are far from full exploitation of the field. One clear reason is that many evaluate immobilization based on only a few experiments that are not always well-designed. In contrast to many other reviews on the subject, here we highlight the pitfalls of using incorrectly designed immobilization protocols and explain why in many cases sub-optimal results are obtained. We also describe solutions to overcome these challenges and come to the conclusion that recent developments in material science, bioprocess engineering and protein science continue to open new opportunities for the future. In this way, enzyme immobilization, far from being a mature discipline, remains as a subject of high interest and where intense research is still necessary to take full advantage of the possibilities.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/102214
url https://hdl.handle.net/20.500.14352/102214
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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