Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings

Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process...

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Autores: Piñuela, Yasmine, Alday, Josu G., Oliach, Daniel, Bolaño, Francesc, Colinas, C. (Carlos), Bonet Lledos, José Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/69758
Acceso en línea:https://doi.org/10.3390/f11080792
http://hdl.handle.net/10459.1/69758
Access Level:acceso abierto
Palabra clave:Black truffle
Truffle colonization
Edible fungi cultivation
Truffle orchard
Truffle trees
Hypogeous ectomycorrhizal fungi
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spelling Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea SaplingsPiñuela, YasmineAlday, Josu G.Oliach, DanielBolaño, FrancescColinas, C. (Carlos)Bonet Lledos, José AntonioBlack truffleTruffle colonizationEdible fungi cultivationTruffle orchardTruffle treesHypogeous ectomycorrhizal fungiResearch Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process and the root development of Portuguese oak (Quercus faginea Lam.) seedlings destined for truffle plantations has not been determined. The main aim of this study was to shed light on the effect of bacterial inoculation on fungal root tip colonization and seedling root traits. Material and methods: We performed a co-inoculation trial using three bacteria naturally present in the T. melanosporum niche (i.e., Pseudomonas fluorescens, Pseudomonas putida, and Bacillus amyloliquefaciens) and two different bacterial inoculation times (one month and nine months after fungal inoculation) under glasshouse conditions. Results: Only P. fluorescens had a significant mycorrhizal promoter effect, increasing the truffle inoculation rates of root tips by more than 10% compared with seedlings that received non-bacterial inoculation treatments. Simultaneously, the co-inoculation of P. fluorescens with T. melanosporum improved seedling root growth parameters compared with those of seedlings that received non-bacterial inoculation treatments. The different bacterial inoculation times and applications of uninoculated bacterial growth media did not affect the root traits analyzed or the root mycorrhization rates. Conclusions: These results suggest that P. fluorescens bacteria have a potential commercial application as a treatment for truffle-inoculated seedlings to improve both seedling quality and mycorrhizal colonization under nursery conditions.This research was funded by the Project INNOVATRUF (PECT El bosc, el primer recurs de l’economia verda—Fons Europeu de Desenvolupament Regional de la Unió Europea-Programa operatiu FEDER de Catalunya 2014-2020) and by the project UdL-Impuls. Y.P. acknowledges the support of University of Lleida for her contract (UdL-Impuls), J.G.A. was supported by the Ramon y Cajal fellowship (RYC-2016-20528), D.O. received support from the ‘Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya’ through the program of ‘Doctorats Industrials’, F.B.’s salary was partially funded by the Ministry of Science, Innovation and Universities through the National Agency of Research (PTA2017-14041-I) and J.A.B. benefitted from a Serra-Húnter Fellowship provided by the Generalitat of Catalunya.MDPI202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/f11080792http://hdl.handle.net/10459.1/69758http://hdl.handle.net/10459.1/69758reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/AEI//PTA2017-14041-IReproducció del document publicat a: https://doi.org/10.3390/f11080792Forests, 2020, vol. 11, num. 8, article 792cc-by (c) Piñuela, Yasmine et al., 2020info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/.oai:recercat.cat:10459.1/697582026-05-29T05:05:01Z
dc.title.none.fl_str_mv Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
title Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
spellingShingle Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
Piñuela, Yasmine
Black truffle
Truffle colonization
Edible fungi cultivation
Truffle orchard
Truffle trees
Hypogeous ectomycorrhizal fungi
title_short Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
title_full Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
title_fullStr Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
title_full_unstemmed Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
title_sort Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
dc.creator.none.fl_str_mv Piñuela, Yasmine
Alday, Josu G.
Oliach, Daniel
Bolaño, Francesc
Colinas, C. (Carlos)
Bonet Lledos, José Antonio
author Piñuela, Yasmine
author_facet Piñuela, Yasmine
Alday, Josu G.
Oliach, Daniel
Bolaño, Francesc
Colinas, C. (Carlos)
Bonet Lledos, José Antonio
author_role author
author2 Alday, Josu G.
Oliach, Daniel
Bolaño, Francesc
Colinas, C. (Carlos)
Bonet Lledos, José Antonio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Black truffle
Truffle colonization
Edible fungi cultivation
Truffle orchard
Truffle trees
Hypogeous ectomycorrhizal fungi
topic Black truffle
Truffle colonization
Edible fungi cultivation
Truffle orchard
Truffle trees
Hypogeous ectomycorrhizal fungi
description Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process and the root development of Portuguese oak (Quercus faginea Lam.) seedlings destined for truffle plantations has not been determined. The main aim of this study was to shed light on the effect of bacterial inoculation on fungal root tip colonization and seedling root traits. Material and methods: We performed a co-inoculation trial using three bacteria naturally present in the T. melanosporum niche (i.e., Pseudomonas fluorescens, Pseudomonas putida, and Bacillus amyloliquefaciens) and two different bacterial inoculation times (one month and nine months after fungal inoculation) under glasshouse conditions. Results: Only P. fluorescens had a significant mycorrhizal promoter effect, increasing the truffle inoculation rates of root tips by more than 10% compared with seedlings that received non-bacterial inoculation treatments. Simultaneously, the co-inoculation of P. fluorescens with T. melanosporum improved seedling root growth parameters compared with those of seedlings that received non-bacterial inoculation treatments. The different bacterial inoculation times and applications of uninoculated bacterial growth media did not affect the root traits analyzed or the root mycorrhization rates. Conclusions: These results suggest that P. fluorescens bacteria have a potential commercial application as a treatment for truffle-inoculated seedlings to improve both seedling quality and mycorrhizal colonization under nursery conditions.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/f11080792
http://hdl.handle.net/10459.1/69758
http://hdl.handle.net/10459.1/69758
url https://doi.org/10.3390/f11080792
http://hdl.handle.net/10459.1/69758
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI//PTA2017-14041-I
Reproducció del document publicat a: https://doi.org/10.3390/f11080792
Forests, 2020, vol. 11, num. 8, article 792
dc.rights.none.fl_str_mv cc-by (c) Piñuela, Yasmine et al., 2020
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/.
rights_invalid_str_mv cc-by (c) Piñuela, Yasmine et al., 2020
http://creativecommons.org/licenses/by/4.0/.
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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