Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation

In several murine models of transplantation, the "cross-dressing" of recipient antigen presenting cells (APCs) with intact donor major histocompatibility complex (MHC) derived from allograft-released small extracellular vesicles (sEVs) has been recently described as a key mechanism in elic...

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Autores: Mastoridis, Sotiris, Londoño, María Carlota, Kurt, Ada, Kodela, Elisavet, Crespo, Elena, Mason, John, Bestard Matamoros, Oriol, Martínez Llordella, Marc, Sánchez Fueyo, Alberto
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/174129
Acceso en línea:https://hdl.handle.net/2445/174129
Access Level:acceso abierto
Palabra clave:Trasplantament de ronyó
Trasplantament hepàtic
Immunologia de la trasplantació
Kidney transplantation
Hepatic transplantation
Transplantation immunology
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spelling Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantationMastoridis, SotirisLondoño, María CarlotaKurt, AdaKodela, ElisavetCrespo, ElenaMason, JohnBestard Matamoros, OriolMartínez Llordella, MarcSánchez Fueyo, AlbertoTrasplantament de ronyóTrasplantament hepàticImmunologia de la trasplantacióKidney transplantationHepatic transplantationTransplantation immunologyIn several murine models of transplantation, the "cross-dressing" of recipient antigen presenting cells (APCs) with intact donor major histocompatibility complex (MHC) derived from allograft-released small extracellular vesicles (sEVs) has been recently described as a key mechanism in eliciting and sustaining alloimmune responses. Investigation of these processes in clinical organ transplantation has, however, been hampered by the lack of sensitivity of conventional instruments and assays. We have employed advanced imaging flow cytometry (iFCM) to explore the kinetics of allograft sEV release and the extent to which donor sEVs might induce cross-dressing following liver and kidney transplantation. We report for the first time that recipient APC cross-dressing can be transiently detected in the circulation shortly after liver, but not kidney, transplantation in association with the release of HLA-bearing allograft-derived sEVs. In liver transplant recipients the majority of circulating cells exhibiting donor HLA are indeed cross-dressed cells and not passenger leukocytes. In keeping with experimental animal data, the downstream functional consequences of the transfer of circulating sEVs harvested from human transplant recipients varies depending on the type of transplant and time posttransplant. sEVs released shortly after liver, but not kidney, transplantation exhibit immunoinhibitory effects that could influence liver allograft immunogenicity.Wiley2020info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2445/174129Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1111/ajt.16123American Journal of Transplantation, 2020https://doi.org/10.1111/ajt.16123cc by (c) Mastoridis, Sotiris et al., 2020http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1741292026-05-27T06:46:51Z
dc.title.none.fl_str_mv Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
title Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
spellingShingle Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
Mastoridis, Sotiris
Trasplantament de ronyó
Trasplantament hepàtic
Immunologia de la trasplantació
Kidney transplantation
Hepatic transplantation
Transplantation immunology
title_short Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
title_full Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
title_fullStr Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
title_full_unstemmed Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
title_sort Impact of donor extracellular vesicle release on recipient cell ‘cross‐dressing’ following clinical liver and kidney transplantation
dc.creator.none.fl_str_mv Mastoridis, Sotiris
Londoño, María Carlota
Kurt, Ada
Kodela, Elisavet
Crespo, Elena
Mason, John
Bestard Matamoros, Oriol
Martínez Llordella, Marc
Sánchez Fueyo, Alberto
author Mastoridis, Sotiris
author_facet Mastoridis, Sotiris
Londoño, María Carlota
Kurt, Ada
Kodela, Elisavet
Crespo, Elena
Mason, John
Bestard Matamoros, Oriol
Martínez Llordella, Marc
Sánchez Fueyo, Alberto
author_role author
author2 Londoño, María Carlota
Kurt, Ada
Kodela, Elisavet
Crespo, Elena
Mason, John
Bestard Matamoros, Oriol
Martínez Llordella, Marc
Sánchez Fueyo, Alberto
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trasplantament de ronyó
Trasplantament hepàtic
Immunologia de la trasplantació
Kidney transplantation
Hepatic transplantation
Transplantation immunology
topic Trasplantament de ronyó
Trasplantament hepàtic
Immunologia de la trasplantació
Kidney transplantation
Hepatic transplantation
Transplantation immunology
description In several murine models of transplantation, the "cross-dressing" of recipient antigen presenting cells (APCs) with intact donor major histocompatibility complex (MHC) derived from allograft-released small extracellular vesicles (sEVs) has been recently described as a key mechanism in eliciting and sustaining alloimmune responses. Investigation of these processes in clinical organ transplantation has, however, been hampered by the lack of sensitivity of conventional instruments and assays. We have employed advanced imaging flow cytometry (iFCM) to explore the kinetics of allograft sEV release and the extent to which donor sEVs might induce cross-dressing following liver and kidney transplantation. We report for the first time that recipient APC cross-dressing can be transiently detected in the circulation shortly after liver, but not kidney, transplantation in association with the release of HLA-bearing allograft-derived sEVs. In liver transplant recipients the majority of circulating cells exhibiting donor HLA are indeed cross-dressed cells and not passenger leukocytes. In keeping with experimental animal data, the downstream functional consequences of the transfer of circulating sEVs harvested from human transplant recipients varies depending on the type of transplant and time posttransplant. sEVs released shortly after liver, but not kidney, transplantation exhibit immunoinhibitory effects that could influence liver allograft immunogenicity.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/174129
url https://hdl.handle.net/2445/174129
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/ajt.16123
American Journal of Transplantation, 2020
https://doi.org/10.1111/ajt.16123
dc.rights.none.fl_str_mv cc by (c) Mastoridis, Sotiris et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Mastoridis, Sotiris et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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