REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration

Zebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the role of...

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Autores: Pérez-Montes, Cristina, Jiménez-Cubides, Jhoana Paola, Velasco, Almudena, Arévalo, Rosario, Santos-Ledo, Adrián, García-Macia, Marina
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/351370
Acesso em linha:http://hdl.handle.net/10261/351370
https://api.elsevier.com/content/abstract/scopus_id/85180432608
Access Level:acceso abierto
Palavra-chave:ROS
Melatonin
Regeneration
Visual system
Zebrafish
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spelling REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System RegenerationPérez-Montes, CristinaJiménez-Cubides, Jhoana PaolaVelasco, AlmudenaArévalo, RosarioSantos-Ledo, AdriánGarcía-Macia, MarinaROSMelatoninRegenerationVisual systemZebrafishZebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the role of oligodendrocytes and the signals that mediate regeneration, we use the well-established optic nerve (ON) crush model. We also used sox10 fluorescent transgenic lines to label fully differentiated oligodendrocytes. To quench the effect of reactive oxygen species (ROS), we used the endogenous antioxidant melatonin. Using these tools, we measured ROS production by flow cytometry and explored the regeneration of the optic tectum (OT), the response of oligodendrocytes and their mitochondria by confocal microscopy and Western blot. ROS are produced by oligodendrocytes 3 h after injury and JNK activity is triggered. Concomitantly, there is a decrease in the number of fully differentiated oligodendrocytes in the OT and in their mitochondrial population. By 24 h, oligodendrocytes partially recover. Exposure to melatonin blocks the changes observed in these oligodendrocytes at 3 h and increases their number and their mitochondrial populations after 24 h. Melatonin also blocks JNK upregulation and induces aberrant neuronal differentiation in the OT. In conclusion, a proper balance of ROS is necessary during visual system regeneration and exposure to melatonin has a detrimental impact.This project was funded by: Lanzadera TCUE21-21_003, C2 program for Universidad de Salamanca; Convocatoria de ayudas de investigación Biomédica 2021, FERP21/003, Fundación Eugenio Rodríguez Pascual; ESCI 2022 Exploratory Research Grants; ESCI22/001, European Society for Clinical Investigation ESCI; Internationalization Project “CL-EI-2021-08-IBFG Unit of Excellence” from Spanish National Research Council (CSIC), funded by the Regional Government of Castile and Leon and co-financed by the European Regional Development Fund (ERDF “Europe drives our growth”). CPM is a USAL fellow Plan Especial Grado Medicina. MGM is a Ramón y Cajal Researcher RYC2021-033684-I, MICIN. We are members of Women in Autophagy (WIA), la Sociedad Española de Bioquímica y Biología Molecular (SEBBM) and la Sociedad Española de Autofagia (SEFAGIA).Peer reviewedMolecular Diversity Preservation InternationalUniversidad de SalamancaFundación Eugenio Rodríguez PascualEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Junta de Castilla y LeónAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Sociedad Española de Bioquímica y Biología MolecularSociedad Española de AutofagiaPérez-Montes, Cristina [0000-0003-1195-9367]García-Macia, Marina [0000-0002-3908-9060]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/351370https://api.elsevier.com/content/abstract/scopus_id/85180432608reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//RYC2021-033684-IThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/antiox12122026https://doi.org/10.3390/antiox12122026Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3513702026-05-22T06:33:51Z
dc.title.none.fl_str_mv REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
title REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
spellingShingle REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
Pérez-Montes, Cristina
ROS
Melatonin
Regeneration
Visual system
Zebrafish
title_short REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
title_full REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
title_fullStr REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
title_full_unstemmed REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
title_sort REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
dc.creator.none.fl_str_mv Pérez-Montes, Cristina
Jiménez-Cubides, Jhoana Paola
Velasco, Almudena
Arévalo, Rosario
Santos-Ledo, Adrián
García-Macia, Marina
author Pérez-Montes, Cristina
author_facet Pérez-Montes, Cristina
Jiménez-Cubides, Jhoana Paola
Velasco, Almudena
Arévalo, Rosario
Santos-Ledo, Adrián
García-Macia, Marina
author_role author
author2 Jiménez-Cubides, Jhoana Paola
Velasco, Almudena
Arévalo, Rosario
Santos-Ledo, Adrián
García-Macia, Marina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Salamanca
Fundación Eugenio Rodríguez Pascual
European Commission
Consejo Superior de Investigaciones Científicas (España)
Junta de Castilla y León
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Sociedad Española de Bioquímica y Biología Molecular
Sociedad Española de Autofagia
Pérez-Montes, Cristina [0000-0003-1195-9367]
García-Macia, Marina [0000-0002-3908-9060]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv ROS
Melatonin
Regeneration
Visual system
Zebrafish
topic ROS
Melatonin
Regeneration
Visual system
Zebrafish
description Zebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the role of oligodendrocytes and the signals that mediate regeneration, we use the well-established optic nerve (ON) crush model. We also used sox10 fluorescent transgenic lines to label fully differentiated oligodendrocytes. To quench the effect of reactive oxygen species (ROS), we used the endogenous antioxidant melatonin. Using these tools, we measured ROS production by flow cytometry and explored the regeneration of the optic tectum (OT), the response of oligodendrocytes and their mitochondria by confocal microscopy and Western blot. ROS are produced by oligodendrocytes 3 h after injury and JNK activity is triggered. Concomitantly, there is a decrease in the number of fully differentiated oligodendrocytes in the OT and in their mitochondrial population. By 24 h, oligodendrocytes partially recover. Exposure to melatonin blocks the changes observed in these oligodendrocytes at 3 h and increases their number and their mitochondrial populations after 24 h. Melatonin also blocks JNK upregulation and induces aberrant neuronal differentiation in the OT. In conclusion, a proper balance of ROS is necessary during visual system regeneration and exposure to melatonin has a detrimental impact.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/351370
https://api.elsevier.com/content/abstract/scopus_id/85180432608
url http://hdl.handle.net/10261/351370
https://api.elsevier.com/content/abstract/scopus_id/85180432608
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//RYC2021-033684-I
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/antiox12122026
https://doi.org/10.3390/antiox12122026
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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