Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition

This article belongs to the Section Crop Breeding and Genetics

Detalles Bibliográficos
Autores: Reveglia, Pierluigi, Barilli, Eleonora, Cobos, María José, López-Orozco, Maria Claudia, Rubiales, Diego
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/417123
Acceso en línea:http://hdl.handle.net/10261/417123
Access Level:acceso abierto
Palabra clave:Pimpinella anisum
Anise
Essential oils
Terpenes
GC-MS
GC-FID
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spelling Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and CompositionReveglia, PierluigiBarilli, EleonoraCobos, María JoséLópez-Orozco, Maria ClaudiaRubiales, DiegoPimpinella anisumAniseEssential oilsTerpenesGC-MSGC-FIDThis article belongs to the Section Crop Breeding and GeneticsAnise (Pimpinella anisum L.) is one of the most important annual herbs of the Apiaceae family, widely cultivated in southern Spain. Their seeds are highly valued for culinary uses and for producing quality essential oils widely used in food and beverage products, as well as for industry, medicinal, and cosmetics applications. This study investigates the seed yield and essential oil content within a set of 50 anise accessions from worldwide origin, as well as their composition by GC–MS and GC–FID analysis. Accessions showed significant differences in the agronomic parameters measured, including plant height (cm), seed yield (kg ha−1), and the Harvest Index (%), with accessions PA_87 (Spain), PA_47 (Greece), and PA_21 (unknown origin) being the most performant. Essential oil (EO) content varied between 0.8% and 5.7% across different genotypes, resulting in EO production values ranging from 0.1 to 300 kg ha−1. Trans-anethole was identified as the dominant terpene, comprising 84.4% to 94.4% of the content, followed by eugenol (1.4% to 5.5%) and α-muurolene (1.4% to 7.2%). PCA analysis identified five distinct groups and one outlier, influenced by minor terpenes. Indeed, there was a strong negative correlation between estragole and pseudoisoeugenyl 2-methylbutyrate. This study underscores the significance of minor terpenes, which play crucial roles in defining unique aniseed chemotypes, allowing for the selection of cultivars optimized for specific uses in food, cosmetics, and pharmaceuticals. Additionally, these findings emphasize the impact of cultivar genetics on agronomic traits and EO profiles, suggesting the need for further research to optimize plant growth and yield and EO quality.This research was funded by the European Union project H2020-RADIANT, project no. 101000622, and by the Junta de Andalucía Qualifica Project QUAL21_023 IAS.Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionJunta de AndalucíaBarilli, Eleonora [0000-0001-6234-6312]Rubiales, Diego [0000-0001-9644-8616]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2026202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/417123reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/101000622The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/agronomy16030285https://doi.org/10.3390/agronomy16030285Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4171232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
title Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
spellingShingle Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
Reveglia, Pierluigi
Pimpinella anisum
Anise
Essential oils
Terpenes
GC-MS
GC-FID
title_short Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
title_full Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
title_fullStr Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
title_full_unstemmed Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
title_sort Characterization of Pimpinella anisum Germplasm: Diversity Available for Agronomic Performance and Essential Oil Content and Composition
dc.creator.none.fl_str_mv Reveglia, Pierluigi
Barilli, Eleonora
Cobos, María José
López-Orozco, Maria Claudia
Rubiales, Diego
author Reveglia, Pierluigi
author_facet Reveglia, Pierluigi
Barilli, Eleonora
Cobos, María José
López-Orozco, Maria Claudia
Rubiales, Diego
author_role author
author2 Barilli, Eleonora
Cobos, María José
López-Orozco, Maria Claudia
Rubiales, Diego
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Junta de Andalucía
Barilli, Eleonora [0000-0001-6234-6312]
Rubiales, Diego [0000-0001-9644-8616]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pimpinella anisum
Anise
Essential oils
Terpenes
GC-MS
GC-FID
topic Pimpinella anisum
Anise
Essential oils
Terpenes
GC-MS
GC-FID
description This article belongs to the Section Crop Breeding and Genetics
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/417123
url http://hdl.handle.net/10261/417123
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/101000622
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/agronomy16030285
https://doi.org/10.3390/agronomy16030285
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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